Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between...
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However, liquid chromatography techniques exist that do utilize affinity chromatography properties. Immobilized metal affinity chromatography (IMAC) is useful...
58 KB (7,243 words) - 02:30, 9 February 2025
affinity Étienne François Geoffroy — Geoffroy's 1718 Affinity Table Valency Affinity chromatography Affinity electrophoresis Chisholm 1911, Affinity,...
10 KB (1,341 words) - 19:24, 14 August 2024
Protein purification (section Affinity chromatography)
can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...
30 KB (3,951 words) - 16:33, 14 April 2025
Chemoproteomics (section Affinity chromatography)
(November 2020). "Affinity chromatography: A review of trends and developments over the past 50 years". Journal of Chromatography. B, Analytical Technologies...
63 KB (6,159 words) - 14:33, 30 November 2024
affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by...
19 KB (2,124 words) - 04:22, 8 April 2024
characteristics, such as acidity, basicity, charge, and affinity for certain compounds. With size exclusion chromatography, there are short and well-defined separation...
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High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used...
89 KB (10,969 words) - 21:02, 3 May 2025
using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC)...
22 KB (2,655 words) - 04:36, 16 October 2024
available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The...
18 KB (2,378 words) - 02:45, 29 March 2025
Meir Wilchek (section Affinity chromatography)
biorecognition or affinity phenomenon, and its various application, e.g. for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin...
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Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly...
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Protein methods (section Affinity Chromatography)
size, and binding affinity. Here are the main types of chromatography used in protein purification: Ion exchange chromatography separates proteins based...
19 KB (2,284 words) - 04:51, 9 November 2024
As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a...
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Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...
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a soft electrophile. This mode of action makes them useful for affinity chromatography to separate thiol-containing compounds from complex mixtures. For...
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Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be...
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separation in either elution or displacement chromatography, there must be appreciable differences in the affinity of the respective solutes for the stationary...
19 KB (2,639 words) - 18:13, 15 December 2023
adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these...
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scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous...
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and carbohydrate structure by means of affinity chromatography, blotting, affinity electrophoresis, and affinity immunoelectrophoreis with lectins, as...
30 KB (3,037 words) - 01:01, 21 May 2025
high-performance liquid chromatography, immunoassay, enzymatic assay, capillary electrophoresis, or boronate affinity chromatography. Point of care (e.g....
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affinity chromatography (IMAC). Imidazole is used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column...
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Protein tag (redirect from Affinity tag)
(TRX) and poly(NANP). Some affinity tags have a dual role as a solubilization agent, such as MBP and GST. Chromatography tags are used to alter chromatographic...
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suggestion of common descent or type Affinity chromatography, method of separating a biomolecule from a mixture Affinity electrophoresis, general name for...
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Size-exclusion chromatography (SEC) Affinity chromatography Centrifugal partition chromatography Gas chromatography and Inverse gas chromatography Crystallization...
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50 μL reaction mixture. Heparin has been used as a chromatography resin, acting as both an affinity ligand and an ion exchanger. Its polyanionic structure...
78 KB (7,850 words) - 05:51, 15 April 2025
monoclonal antibodies are often purified using Protein A/G or antigen-affinity chromatography. In research, purified antibodies are used in many applications...
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or a hexa-his peptide (6xHis-tag), which can be isolated using affinity chromatography with nickel or cobalt resins. Di- or multimeric chimeric proteins...
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Glycome (section High-resolution mass spectrometry (MS) and high-performance liquid chromatography (HPLC))
The purity of test samples have been obtained through chromatography (affinity chromatography etc.) and analytical electrophoresis (PAGE (polyacrylamide...
13 KB (1,501 words) - 03:42, 24 March 2024