• Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between...
    29 KB (3,430 words) - 03:30, 24 June 2024
  • However, liquid chromatography techniques exist that do utilize affinity chromatography properties. Immobilized metal affinity chromatography (IMAC) is useful...
    58 KB (7,243 words) - 02:30, 9 February 2025
  • affinity Étienne François Geoffroy — Geoffroy's 1718 Affinity Table Valency Affinity chromatography Affinity electrophoresis Chisholm 1911, Affinity,...
    10 KB (1,341 words) - 19:24, 14 August 2024
  • can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...
    30 KB (3,951 words) - 16:33, 14 April 2025
  • (November 2020). "Affinity chromatography: A review of trends and developments over the past 50 years". Journal of Chromatography. B, Analytical Technologies...
    63 KB (6,159 words) - 14:33, 30 November 2024
  • Thumbnail for Affinity electrophoresis
    affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by...
    19 KB (2,124 words) - 04:22, 8 April 2024
  • Thumbnail for Size-exclusion chromatography
    characteristics, such as acidity, basicity, charge, and affinity for certain compounds. With size exclusion chromatography, there are short and well-defined separation...
    31 KB (3,901 words) - 18:48, 7 April 2025
  • Thumbnail for High-performance liquid chromatography
    High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used...
    89 KB (10,969 words) - 21:02, 3 May 2025
  • Thumbnail for His-tag
    using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC)...
    22 KB (2,655 words) - 04:36, 16 October 2024
  • Thumbnail for Column chromatography
    available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The...
    18 KB (2,378 words) - 02:45, 29 March 2025
  • Thumbnail for Meir Wilchek
    biorecognition or affinity phenomenon, and its various application, e.g. for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin...
    15 KB (1,665 words) - 13:07, 5 February 2025
  • Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly...
    8 KB (1,054 words) - 16:14, 13 March 2025
  • size, and binding affinity. Here are the main types of chromatography used in protein purification: Ion exchange chromatography separates proteins based...
    19 KB (2,284 words) - 04:51, 9 November 2024
  • Thumbnail for Fast protein liquid chromatography
    As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a...
    16 KB (2,274 words) - 22:20, 25 November 2024
  • Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...
    11 KB (1,327 words) - 11:09, 21 January 2025
  • Thumbnail for Organomercury chemistry
    a soft electrophile. This mode of action makes them useful for affinity chromatography to separate thiol-containing compounds from complex mixtures. For...
    16 KB (1,667 words) - 03:34, 27 April 2025
  • Thumbnail for Paper chromatography
    Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be...
    8 KB (1,099 words) - 08:45, 3 April 2025
  • separation in either elution or displacement chromatography, there must be appreciable differences in the affinity of the respective solutes for the stationary...
    19 KB (2,639 words) - 18:13, 15 December 2023
  • Thumbnail for Liquid chromatography–mass spectrometry
    adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these...
    46 KB (5,943 words) - 04:07, 12 May 2025
  • Thumbnail for Agarose
    scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous...
    20 KB (2,443 words) - 17:35, 19 September 2024
  • Thumbnail for Lectin
    and carbohydrate structure by means of affinity chromatography, blotting, affinity electrophoresis, and affinity immunoelectrophoreis with lectins, as...
    30 KB (3,037 words) - 01:01, 21 May 2025
  • high-performance liquid chromatography, immunoassay, enzymatic assay, capillary electrophoresis, or boronate affinity chromatography. Point of care (e.g....
    44 KB (4,974 words) - 23:26, 12 May 2025
  • Thumbnail for Imidazole
    affinity chromatography (IMAC). Imidazole is used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column...
    27 KB (2,716 words) - 04:50, 5 February 2025
  • Protein tag (redirect from Affinity tag)
    (TRX) and poly(NANP). Some affinity tags have a dual role as a solubilization agent, such as MBP and GST. Chromatography tags are used to alter chromatographic...
    24 KB (2,683 words) - 16:18, 22 March 2025
  • suggestion of common descent or type Affinity chromatography, method of separating a biomolecule from a mixture Affinity electrophoresis, general name for...
    4 KB (440 words) - 19:13, 8 November 2023
  • Size-exclusion chromatography (SEC) Affinity chromatography Centrifugal partition chromatography Gas chromatography and Inverse gas chromatography Crystallization...
    8 KB (936 words) - 12:57, 18 May 2025
  • Thumbnail for Heparin
    50 μL reaction mixture. Heparin has been used as a chromatography resin, acting as both an affinity ligand and an ion exchanger. Its polyanionic structure...
    78 KB (7,850 words) - 05:51, 15 April 2025
  • Thumbnail for Antibody
    monoclonal antibodies are often purified using Protein A/G or antigen-affinity chromatography. In research, purified antibodies are used in many applications...
    126 KB (13,957 words) - 01:37, 19 March 2025
  • Thumbnail for Fusion protein
    or a hexa-his peptide (6xHis-tag), which can be isolated using affinity chromatography with nickel or cobalt resins. Di- or multimeric chimeric proteins...
    19 KB (2,305 words) - 05:22, 15 May 2024
  • Thumbnail for Glycome
    The purity of test samples have been obtained through chromatography (affinity chromatography etc.) and analytical electrophoresis (PAGE (polyacrylamide...
    13 KB (1,501 words) - 03:42, 24 March 2024