Helicase-dependent amplification (HDA) is a method for in vitro DNA amplification (like the polymerase chain reaction) that takes place at a constant...
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Polymerase chain reaction (redirect from PCR amplification)
proportion of negative outcomes. Hence the name 'digital PCR'. Helicase-dependent amplification: similar to traditional PCR, but uses a constant temperature...
90 KB (11,293 words) - 13:08, 23 May 2025
other isothermal amplification techniques include LAMP, NASBA, helicase-dependent amplification (HDA), and nicking enzyme amplification reaction (NEAR)...
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Variants of PCR (redirect from Isothermal amplification)
groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (MLPA) permits multiple targets to be amplified using only a single pair of primers...
33 KB (4,155 words) - 13:49, 10 March 2025
Naming conventions Head and Disk Assembly of a Winchester disk Helicase-dependent amplification High density amorphous ice Higher-dimensional algebra Dragonair...
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DNA replication (redirect from Amplification of DNA)
DNA helicases for leading strands and lagging strands are loaded on the template DNAs, the helicases run along the DNAs into each other. The helicases remain...
60 KB (7,447 words) - 05:04, 1 June 2025
Φ29 DNA polymerase (section Whole genome amplification)
increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable...
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archaeal replicative helicase core, Mcm2-7 is loaded as a head-to-head double hexamer onto DNA to license origins. In S-phase, Dbf4-dependent kinase (DDK) and...
113 KB (12,563 words) - 03:12, 16 December 2024
eukaryotes. This pathway includes the Mus81 resolvase and the Sgs1 helicase. The Sgs1 helicase appears to promote the non-crossover outcome of meiotic recombinational...
34 KB (3,803 words) - 09:15, 23 March 2025
picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease and an RNA-dependent RNA polymerase. VP1 is encoded between codons...
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ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp...
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hairpin called the origin and establishing a replication fork with its helicase activity. Nicking leads to the hairpin unfolding into a linear, extended...
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domain architecture. All contain a catalytic helicase core made up of two RecA-like domains. The catalytic helicase core contains at least 9 highly conserved...
25 KB (3,011 words) - 17:07, 24 May 2024
"Homologous recombination via synthesis-dependent strand annealing in yeast requires the Irc20 and Srs2 DNA helicases". Genetics. 191 (1): 65–78. doi:10.1534/genetics...
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encodes for a combined protein that has methyltransferase, helicase, and an RNA-dependent RNA polymerase domains. RNA-2 is 2,231 nucleotides in length...
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biosynthesis (in vivo DNA amplification), polymerase chain reaction - enzymatic DNA synthesis (in vitro DNA amplification) or gene synthesis - physically...
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DNA polymerase (redirect from DNA-dependent DNA polymerase)
generation to generation. Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form, in the process breaking...
59 KB (7,106 words) - 00:05, 4 June 2025
replicative factors associate with the pre-RC. Stable association encourages MCM helicase to unwind a small stretch of parental DNA into two strands of ssDNA, which...
15 KB (1,681 words) - 15:45, 29 October 2023
proteins is known, NS5 serves as RNA-dependent RNA polymerase, NS3 has protease (in complex with NS2B) and helicase activity. Structural and nonstructural...
23 KB (2,724 words) - 00:35, 13 March 2025
exogenous RNA including toll-like receptor 3,7,8 (TLR3, TLR7, TLR8), the RNA helicase RIG1 (RARRES3), protein kinase R (PKR, a.k.a. EIF2AK2), members of the...
34 KB (4,131 words) - 09:48, 4 June 2025
Siksnys V (April 2011). "Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system". The EMBO Journal. 30 (7): 1335–1342...
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transcribed and melts or unwinds the DNA (ATP-dependent) to separate the two strands using its helicase activity. It has a kinase activity that phosphorylates...
37 KB (4,622 words) - 17:05, 6 April 2024
such as 5-FU. It has been shown that MSI-H cancers are dependent on the Werner syndrome helicase (WRN) to repair the DNA secondary structures that are...
26 KB (3,138 words) - 21:31, 29 May 2025
Argonaute complex recruits an RNA-dependent RNA polymerase (RdRP) to generate secondary siRNAs, triggering an amplification response. Secondary siRNAs are...
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premature aging disease that is associated with early onset of cancer. WRN helicase, which is mutated in Werner Syndrome patients, is required for efficient...
11 KB (1,152 words) - 09:54, 28 May 2025
(2008-01-24). "Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein"...
65 KB (7,496 words) - 05:28, 28 May 2025
hereafter. nsP1 is methyltransferase and guanyltransferase. nsP2 is a helicase and protease, and it interferes with the host immune response. nsP3 is...
49 KB (6,165 words) - 21:49, 13 May 2025
decarboxylase (ADC) BCAS2: Breast carcinoma amplified sequence 2 BCL10 (1p22) LRIF1: encoding protein Ligand-dependent nuclear receptor-interacting factor 1...
47 KB (3,307 words) - 00:15, 1 June 2025
5-bisphosphate carboxylase/oxygenase.[citation needed] CI (~71 kDa) is an RNA helicase with ATPase activity. Its most unusual property is its ability to form...
46 KB (5,375 words) - 17:21, 23 May 2025
near the core AAA fold: clamp loader, initiator, classic, superfamily III helicase, HCLR, H2-insert, and PS-II insert. AAA ATPases assemble into oligomeric...
14 KB (1,588 words) - 07:52, 28 March 2025