• Helicase-dependent amplification (HDA) is a method for in vitro DNA amplification (like the polymerase chain reaction) that takes place at a constant...
    6 KB (748 words) - 21:52, 19 October 2024
  • Thumbnail for Polymerase chain reaction
    proportion of negative outcomes. Hence the name 'digital PCR'. Helicase-dependent amplification: similar to traditional PCR, but uses a constant temperature...
    90 KB (11,293 words) - 13:08, 23 May 2025
  • other isothermal amplification techniques include LAMP, NASBA, helicase-dependent amplification (HDA), and nicking enzyme amplification reaction (NEAR)...
    14 KB (1,707 words) - 15:45, 22 October 2024
  • groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (MLPA) permits multiple targets to be amplified using only a single pair of primers...
    33 KB (4,155 words) - 13:49, 10 March 2025
  • Naming conventions Head and Disk Assembly of a Winchester disk Helicase-dependent amplification High density amorphous ice Higher-dimensional algebra Dragonair...
    702 bytes (97 words) - 21:29, 26 January 2025
  • Thumbnail for DNA replication
    DNA helicases for leading strands and lagging strands are loaded on the template DNAs, the helicases run along the DNAs into each other. The helicases remain...
    60 KB (7,447 words) - 05:04, 1 June 2025
  • increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable...
    24 KB (3,226 words) - 02:24, 1 June 2025
  • Thumbnail for Origin of replication
    archaeal replicative helicase core, Mcm2-7 is loaded as a head-to-head double hexamer onto DNA to license origins. In S-phase, Dbf4-dependent kinase (DDK) and...
    113 KB (12,563 words) - 03:12, 16 December 2024
  • Thumbnail for Tetrahymena
    eukaryotes. This pathway includes the Mus81 resolvase and the Sgs1 helicase. The Sgs1 helicase appears to promote the non-crossover outcome of meiotic recombinational...
    34 KB (3,803 words) - 09:15, 23 March 2025
  • Thumbnail for Deformed wing virus
    picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease and an RNA-dependent RNA polymerase. VP1 is encoded between codons...
    17 KB (2,226 words) - 01:20, 21 March 2025
  • Thumbnail for DDX1
    ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp...
    8 KB (990 words) - 05:27, 28 March 2024
  • hairpin called the origin and establishing a replication fork with its helicase activity. Nicking leads to the hairpin unfolding into a linear, extended...
    54 KB (7,421 words) - 14:08, 2 November 2024
  • domain architecture. All contain a catalytic helicase core made up of two RecA-like domains. The catalytic helicase core contains at least 9 highly conserved...
    25 KB (3,011 words) - 17:07, 24 May 2024
  • Thumbnail for Double-strand break repair model
    "Homologous recombination via synthesis-dependent strand annealing in yeast requires the Irc20 and Srs2 DNA helicases". Genetics. 191 (1): 65–78. doi:10.1534/genetics...
    41 KB (5,013 words) - 02:11, 14 May 2025
  • encodes for a combined protein that has methyltransferase, helicase, and an RNA-dependent RNA polymerase domains. RNA-2 is 2,231 nucleotides in length...
    10 KB (1,038 words) - 07:28, 13 August 2023
  • Thumbnail for DNA synthesis
    biosynthesis (in vivo DNA amplification), polymerase chain reaction - enzymatic DNA synthesis (in vitro DNA amplification) or gene synthesis - physically...
    14 KB (1,820 words) - 18:46, 3 May 2025
  • Thumbnail for DNA polymerase
    generation to generation. Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form, in the process breaking...
    59 KB (7,106 words) - 00:05, 4 June 2025
  • Thumbnail for S phase
    replicative factors associate with the pre-RC. Stable association encourages MCM helicase to unwind a small stretch of parental DNA into two strands of ssDNA, which...
    15 KB (1,681 words) - 15:45, 29 October 2023
  • Thumbnail for Tick-borne encephalitis virus
    proteins is known, NS5 serves as RNA-dependent RNA polymerase, NS3 has protease (in complex with NS2B) and helicase activity. Structural and nonstructural...
    23 KB (2,724 words) - 00:35, 13 March 2025
  • exogenous RNA including toll-like receptor 3,7,8 (TLR3, TLR7, TLR8), the RNA helicase RIG1 (RARRES3), protein kinase R (PKR, a.k.a. EIF2AK2), members of the...
    34 KB (4,131 words) - 09:48, 4 June 2025
  • Thumbnail for CRISPR
    Siksnys V (April 2011). "Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system". The EMBO Journal. 30 (7): 1335–1342...
    115 KB (11,900 words) - 10:27, 3 June 2025
  • transcribed and melts or unwinds the DNA (ATP-dependent) to separate the two strands using its helicase activity. It has a kinase activity that phosphorylates...
    37 KB (4,622 words) - 17:05, 6 April 2024
  • Thumbnail for Microsatellite instability
    such as 5-FU. It has been shown that MSI-H cancers are dependent on the Werner syndrome helicase (WRN) to repair the DNA secondary structures that are...
    26 KB (3,138 words) - 21:31, 29 May 2025
  • Argonaute complex recruits an RNA-dependent RNA polymerase (RdRP) to generate secondary siRNAs, triggering an amplification response. Secondary siRNAs are...
    8 KB (941 words) - 11:41, 13 August 2023
  • Thumbnail for Jan Karlseder
    premature aging disease that is associated with early onset of cancer. WRN helicase, which is mutated in Werner Syndrome patients, is required for efficient...
    11 KB (1,152 words) - 09:54, 28 May 2025
  • Thumbnail for EIF4E
    (2008-01-24). "Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein"...
    65 KB (7,496 words) - 05:28, 28 May 2025
  • hereafter. nsP1 is methyltransferase and guanyltransferase. nsP2 is a helicase and protease, and it interferes with the host immune response. nsP3 is...
    49 KB (6,165 words) - 21:49, 13 May 2025
  • Thumbnail for Chromosome 1
    decarboxylase (ADC) BCAS2: Breast carcinoma amplified sequence 2 BCL10 (1p22) LRIF1: encoding protein Ligand-dependent nuclear receptor-interacting factor 1...
    47 KB (3,307 words) - 00:15, 1 June 2025
  • Thumbnail for Potyvirus
    5-bisphosphate carboxylase/oxygenase.[citation needed] CI (~71 kDa) is an RNA helicase with ATPase activity. Its most unusual property is its ability to form...
    46 KB (5,375 words) - 17:21, 23 May 2025
  • Thumbnail for AAA proteins
    near the core AAA fold: clamp loader, initiator, classic, superfamily III helicase, HCLR, H2-insert, and PS-II insert. AAA ATPases assemble into oligomeric...
    14 KB (1,588 words) - 07:52, 28 March 2025