A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules...

Lysis (/ˈlaɪsɪs/ LY-sis; from Greek λῠ́σῐς lýsis 'loosening') is the breaking down of the membrane of a cell, often by viral, enzymic, or osmotic (that...

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). This buffer is more denaturing...

changes in pH Lysis buffer, in cell biology Metal ion buffer Mineral redox buffer, in geology Buffer (GIS), a zone around a map feature Buffer (optical fiber)...

Buffer P2 is a lysis buffer solution produced by Qiagen. It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and...

chloride solutions with ammonia are used as buffer solutions including ACK (Ammonium-Chloride-Potassium) lysis buffer. In paleontology, ammonium chloride vapor...

zwitterionic detergents such as CHAPS Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction Reducing surface tension...

into the glass cylinder, alongside a suitable volume of an appropriate lysis buffer. Homogenization is performed by a defined number of "passes" of the pestles...

The steps of alkaline lysis can be summarized as the formation of a pellet, resuspension of the pellet in solution, cell lysis, neutralization, and centrifugation...

Besides that, the ideal lysis buffer is needed to acquire substantial amounts of target protein content because the buffer is leading the process of...

of the Anatomical Therapeutic Chemical Classification System Buffer P2, a lysis buffer solution Period 2, of the periodic table Pollution prevention...

is resuspended in a lysis buffer typically containing lysozyme and sometimes a detergent such as LDAO or Triton X-100. This buffer disintegrates the cell...

Alternatively, one or more protease inhibitors can be added to the lysis buffer immediately before cell disruption. Sometimes it is also necessary to...

laser capture microdissection, where cells are collected directly into lysis buffer and cDNA is generated without the need for RNA isolation, which both...

suspension of cells and the other containing the barcoded beads suspended in lysis buffer) that both flow into an oil channel. Laminar flow prevents the mixing...

several methods for cell lysis, including alkaline lysis, mechanical lysis, and enzymatic lysis. The most common method is alkaline lysis, which involves the...

application, the most common methods are: mechanical, chemical and enzymatic lysis, precipitation, purification, and concentration. The specific method used...

locus - luminescent protein - lymphocyte homing receptor - lysine - lysis - lysis buffer - lysozyme - lytic cycle macroevolution - macromolecular system -...

the pellet is re-suspended in PBS and incubated with a red blood cell lysis buffer. Muse cells isolated by this method have been found to be distinct population...

within one hour from sample collection. Samples are collected in a viral lysis buffer that require very minimal biosafety and biosecurity. The Lancet remarked...

sometimes added at relatively high concentrations (~20 mM) to protein lysis buffers in order to inhibit endogenous phosphatases and thereby protect phosphorylated...

site thiol group (see schematic). NEM blocks vesicular transport. In lysis buffers, 20 to 25 mM of NEM is used to inhibit de-sumoylation of proteins for...

fluorescence-activated cell sorting (FACS). The cells are directly sorted into lysis buffer and their DNA is extracted. Having been arrested at a specified number...

cells collected through any of the above means are lysed with a cell lysis buffer to obtain high protein concentration. Aliquots of the lysates are pooled...

method are lyse, bind, wash, and elute. More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid...

next step of the procedure. In situ digestion involves cell lysis with the use of a lysis buffer followed by digestion with a restriction enzyme MboI. This...

with a pH 11.8 alkaline lysis buffer, neutralized with a neutralization buffer, and precipitated using a precipitation buffer. A commercial plasmid purification...

Dulbecco's phosphate-buffered saline (DPBS), trypsin or dispase (depending on cell type), trypan blue, hemocytometer, lysis buffer, and wash buffer To treat nuclei...

possible to isolate individual cells and beads together in droplets of lysis buffer, where the polyadenylation site binds to a primer containing a unique...

leading to the lysis of cells that are sensitive to complement. In the sucrose lysis test, patient RBCs are washed and resuspended in a buffered sucrose solution...