Nested polymerase chain reaction (nested PCR) is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to...
3 KB (361 words) - 16:20, 23 October 2023
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...
90 KB (11,293 words) - 13:08, 23 May 2025
The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have...
16 KB (2,030 words) - 10:07, 20 June 2024
chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by nested polymerase chain reaction". J Med Entomol. 38 (2): 308–311. doi:10.1603/0022-2585-38...
29 KB (3,268 words) - 21:31, 15 June 2025
chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by nested polymerase chain reaction". Journal of Medical Entomology. 38 (2): 308–311. doi:10.1603/0022-2585-38...
48 KB (5,036 words) - 16:38, 8 June 2025
Variants of PCR (category Polymerase chain reaction)
diverse array of techniques that have evolved from the basic polymerase chain reaction (PCR) method, each tailored to specific applications in molecular...
33 KB (4,155 words) - 13:49, 10 March 2025
proteins generated by an infected host in response to the infection. Polymerase chain reaction (PCR) assays are the most commonly used molecular technique to...
38 KB (4,347 words) - 05:13, 26 May 2025
Cell-free fetal DNA (section Nested PCR)
gestation or more, both segments of DNA were found. The use of nested polymerase chain reaction (nested PCR) was evaluated to determine sex by detecting a Y chromosome...
51 KB (5,960 words) - 01:34, 16 June 2025
microscopy and spectroscopy. Some other methods including DNA-based polymerase chain reactions, protein-based two-dimension gel electrophoresis and genetic identification...
25 KB (2,778 words) - 02:34, 2 June 2025
with Parelaphostrongylus species by histology and specific nested polymerase chain reaction in domestic camelids and wild ungulates". Journal of Veterinary...
21 KB (2,443 words) - 10:33, 24 May 2025
COLD-PCR (category Polymerase chain reaction)
(co-amplification at lower denaturation temperature PCR) is a modified polymerase chain reaction (PCR) protocol that enriches variant alleles from a mixture of...
12 KB (1,607 words) - 10:43, 9 July 2024
skin-dwelling filariae M. streptocerca and Onchocerca volvulus, a nested polymerase chain reaction (PCR) assay was developed using small amounts of parasite material...
14 KB (1,483 words) - 04:43, 13 June 2025
GM, Thomas EJ (September 1992). "Reverse transcription with nested polymerase chain reaction shows expression of basic fibroblast growth factor transcripts...
22 KB (2,698 words) - 15:56, 25 May 2025
Bisulfite sequencing (redirect from Methylation-specific polymerase chain reaction)
methylation-specific PCR (MSP) (Figure 4), and strategies employing polymerase chain reaction (PCR) performed under non-methylation-specific conditions (Figure...
35 KB (4,229 words) - 12:59, 12 June 2025
using polymerase chain reaction. This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse...
11 KB (1,346 words) - 12:41, 4 January 2024
hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR)". Diseases of Aquatic Organisms. 63 (2–3): 255–259. doi:10...
21 KB (2,142 words) - 18:26, 22 May 2025
demonstrated by immunophenotyping, in situ hybridization and polymerase chain reaction". American Journal of Clinical Pathology. 103 (4): 449–52. doi:10...
4 KB (309 words) - 11:07, 3 November 2023
malignancies and malignant effusions detected by nested reverse transcriptase-polymerase chain reaction". Laboratory Investigation. 82 (9): 1147–53. doi:10...
1 KB (162 words) - 03:52, 16 June 2024
polyacrylamide gel - polyclonal antibodies - polydactyly - polymerase - polymerase chain reaction - polymorphism - polynucleotide kinase - polypeptide - polyvinyl-alcohol...
23 KB (1,969 words) - 13:07, 28 September 2024
delays in diagnosis and therapy. Amoeba cultures and real-time polymerase chain reaction (PCR) studies for N. fowleri are diagnostic of PAM, but they are...
34 KB (3,557 words) - 19:31, 6 June 2025
specialized equipment and trained human resources. Among these, polymerase chain reaction (PCR) is an accurate, economical and convenient method, which...
19 KB (2,095 words) - 13:02, 28 February 2025
substrate was used to lower the reaction rate and accumulate an intermediate for characterization. The amino acid side-chains glutamic acid 35 (Glu35) and...
38 KB (4,298 words) - 17:01, 11 June 2025
chain-termination method or 'Sanger method', which is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during...
79 KB (7,644 words) - 14:46, 31 March 2025
larger laboratories, lymph collected from a blister is tested by polymerase chain reaction (PCR) for VZV DNA, or examined with an electron microscope for...
80 KB (8,856 words) - 17:36, 7 June 2025
detection threshold that can be detected by reverse transcription polymerase chain reaction targeting gametocyte-specific mRNA. Although it is not one of...
10 KB (1,205 words) - 02:34, 19 June 2025
antibody testing, viral cultures, or real-time reverse transcription polymerase chain reaction may be needed. There is no specific treatment for mumps, so treatment...
63 KB (7,859 words) - 02:48, 13 June 2025
amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain reaction or ligase...
336 KB (31,278 words) - 03:46, 17 June 2025
messenger ribonucleic acid in normal human leukocytes by polymerase chain reaction amplification with nested primers". Lab. Invest. 69 (6): 743–9. PMID 8264236...
10 KB (1,156 words) - 00:19, 27 January 2024
variety of tests for the presence of BFDV are available: standard polymerase chain reaction (PCR), quantitative PCR (qPCR) which can detect the virus in extremely...
40 KB (4,438 words) - 01:51, 7 May 2025
responsible for carrying out the chemical reactions to add new amino acids to a growing polypeptide chain by the formation of peptide bonds. The genetic...
100 KB (11,208 words) - 22:21, 21 April 2025