The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by...

The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...

including unidirectional ligation or concatemerization, overlap extension polymerase chain reaction (OEPCR), and recursive directional ligation (RDL). Also...

many modern televisions Splicing by overlap extension polymerase chain reaction, a variant of polymerase chain reaction Standard Operating Environment, computer...

diverse array of techniques that have evolved from the basic polymerase chain reaction (PCR) method, each tailored to specific applications in molecular...

at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction (LCR), and transcription-mediated amplification...

A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...

electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being...

Vectorette PCR is a variation of polymerase chain reaction (PCR) designed in 1988. The original PCR was created and also patented during the 1980s. Vectorette...

Repair Cloning is a particular form of the polymerase chain reaction in which specialized primers with extensions beyond the sequence of the DNA target are...

Repair Cloning is a particular form of the Polymerase Chain Reaction in which specialized primers with extensions beyond the sequence of the DNA target are...

manipulate it in the laboratory, such as restriction digests and the polymerase chain reaction. Modern biology and biochemistry make intensive use of these techniques...

cDNA molecule is then synthesized through a process called PCR (Polymerase Chain Reaction), which amplifies the cDNA to produce multiple copies. 3)Sequencing:...

isolation. The number of copies of the gene is then amplified using polymerase chain reaction (PCR). Simultaneously, the same restriction enzymes are used to...

restriction enzymes or integrases. Other similar overlap-based assembly methods include Circular Polymerase Extension Cloning (CPEC), Sequence and Ligase Independent...

amplified with the polymerase chain reaction (PCR) by using DNase I, which randomly cleaves DNA. He then completed a modified PCR reaction where primers were...

chain-termination method or 'Sanger method', which is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during...

are milled to a powder and treated with a solution before the polymerase chain reaction (PCR) process. Samples for DNA amplification may not necessarily...

concentration of Taq polymerase. Increase extension time. Increase cycle time. Use less accurate Taq polymerase. Also see polymerase chain reaction for more information...

more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication...

in the parasite genome. The advancement in technologies like polymerase chain reaction (PCR) led to the development of various assays that made large...

specialized polymerases to bypass the lesion and resume DNA replication. After translesion synthesis, extension is required. This extension can be carried...

amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain reaction or ligase...

After melting, the reaction is immediately quenched to 0 °C, and MALBAC primers are added to the reaction. Extension – Bst DNA Polymerase (Large Fragment)...

molecular diagnostics methods based on nucleic acids detection use polymerase chain reaction (PCR) to vastly increase the number of nucleic acid molecules...

traits largely, but not completely, overlap the classifications based on color, ATPase, or MHC (myosin heavy chain). Some authors define a fast twitch...

in SRS is polymerase chain reaction which causes preferential amplification of repetitive DNA. SRS also fails to generate sufficient overlap sequence from...

characteristics. Around the same time the development of effective polymerase chain reaction techniques made it possible to apply cladistic methods of analysis...

necessary for reliable analyses. With the development of the Polymerase Chain Reaction (PCR) in 1983, scientists could study DNA samples up to approximately...

polymer A large macromolecule composed of many repeated subunits. polymerase chain reaction (PCR) A technique used in molecular biology to amplify a single...