A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...

A primer is a short, single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. A synthetic primer is a type of oligo...

bands on PCR gels. Primer dimer formation often competes with formation of the DNA fragment of interest, and may be avoided using primers that are designed...

all dsDNA PCR products, including nonspecific PCR products (such as primer dimer). This can potentially interfere with, or prevent, accurate monitoring...

that utilizes primers with a 3' extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for...

the mixture, are the cause behind the synthesis of by-products such as primer dimer and mis-priming. Mis-priming greatly impedes and reduces the efficiency...

which uses only forward primers, while the second reaction uses only reverse primers. This avoids the formation of primer dimer formation.[page needed]...

reduced primer dimer formation, detection of alternative splicing variants, ability to perform multiplex PCR with higher numbers of PCR primers, and the...

added to view LAMP in real-time. However, in the late amplification, primer-dimer amplification may contribute to a false positive signal. The use of inorganic...

unfamiliar with PCR troubleshooting, as non-specific amplification, primer-dimer artifacts, or DNA degradation can all affect result quality. Moreover...

facilitates high specificity of the primers and reduces the production of nonspecific products, such as primer dimer. Also, use of a thermostable polymerase...

using DNA binding dyes such as SYBR Green since the elimination of primer-dimers can be achieved through a simple change in the melting temperature....

not used at all, and is instead replaced with uracil. thymine dimer See pyrimidine dimer. tissue In a multicellular organism, a contiguous aggregation...

PCR (rhPCR) can reduce primer-dimer formation, and increase the number of assays in multiplex PCR. The method utilizes primers with a cleavable block...

reaction. The PCR products are purified again using AMPure beads to remove primer dimers and then quantified before being sequenced. Regions of chromatin that...

John Primer (born March 5, 1945, Camden, Mississippi, United States) is an American Chicago blues and electric blues singer and guitarist who played behind...

PCR artifacts (e.g. primer-dimers or truncated products) can increase the background noise and obscure the signal. Primer-dimers can also inhibit the...

needed. The genomic RNA of retroviruses is linked non-covalently to the dimer linkage structure (DLS), a non-coding region in the 5' UTR. For the kissing...

Retrieved 2021-04-20. "HostDime obtiene el primer certificado ISO 27701 emitido en Brasil". datacenterdynamics.com. 2020-12-15. "HostDime begins work on Colombian...

PCR reaction. NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins, self and cross dimers, runs and repeats...

balance, resulting in inefficient ligation and an excess amount of primer dimers, respectively. Therefore, it is important to keep the molar concentration...

asymmetric dimer with DNA polymerase epsilon.) This polymerase synthesizes leading strand DNA in eukaryotes. (Thought to form an asymmetric dimer with DNA...

The Oxford Chemistry Primers are a series of short texts providing accounts of a range of essential topics in chemistry and chemical engineering written...

Findings may include low platelets, low fibrinogen, high INR, or high D-dimer. Treatment is mainly directed towards the underlying condition. Other measures...

structure of DnaB is really a trimer of dimers. Both Ile119 and Ile125 are buried in the N-terminal domain dimer interface of DnaB and mutation of these...

stem and the 3D domains (VPg domain) contacting the lower stem. The 3C dimer opens the RNA stem by forming a more stable interaction with single strands...

molecules 7–10 kilobases in length. The two molecules are present as a dimer, formed by base pairing between complementary sequences. Interaction sites...

pathways in which dimer or other oligomers are the reactive species have also been discovered, and for lithium amides such as LDA, dimer-based reactions...

returned to sign a deal with Chilevisión (CHV), with successful programs like Primer Plano, Allá tú, Fiebre de baile (Dance Fever), El Poder del 10 and Talento...

originally linked the two nucleotides of the dimer. Subsequent steps in the repair process involve removal of the dimer remnants and repair synthesis to fill...