• Thumbnail for Real-time polymerase chain reaction
    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
    43 KB (5,360 words) - 20:54, 17 February 2025
  • Thumbnail for Reverse transcription polymerase chain reaction
    technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
    45 KB (5,776 words) - 17:52, 21 March 2025
  • beyond that of real-time PCR. Point mutations, loss of heterozygosity and aneuploidy can be detected in a single PCR step. Digital PCR can differentiate...
    51 KB (5,957 words) - 01:14, 15 January 2025
  • Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments and...
    20 KB (2,673 words) - 23:19, 8 April 2025
  • Thumbnail for Polymerase chain reaction
    real time. It is also sometimes abbreviated to RT-PCR (real-time PCR) but this abbreviation should be used only for reverse transcription PCR. qPCR is...
    90 KB (11,271 words) - 23:49, 1 May 2025
  • Thumbnail for Quantitative PCR instrument
    A quantitative PCR instrument, also called real-time PCR machine, is an analytical instrument that amplifies and detects DNA. It combines the functions...
    11 KB (1,110 words) - 13:24, 3 April 2025
  • Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction...
    69 KB (7,351 words) - 04:46, 30 October 2024
  • for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent...
    5 KB (691 words) - 16:02, 17 September 2024
  • a pH buffer and come mixed in nuclease-free water. Master mixes for real-time PCR include a fluorescent compound (frequently SYBR green), and the choice...
    3 KB (273 words) - 01:11, 30 April 2025
  • Thumbnail for Gene doping
    limited capacity. Therefore, PCR can target these exon-exon junctions as a unique sequence that is not present in gDNA PCR has many applications in molecular...
    32 KB (3,911 words) - 13:42, 10 March 2025
  • Thumbnail for Lyssavirus
    reverse transcription PCR (RT-PCR) tests have been developed for rabies but only been used as a confirmatory test. Real-time PCR-based tests which have...
    14 KB (1,448 words) - 22:47, 15 March 2025
  • prior to PCR testing. Marth, Elmer H. (27 March 2007). Listeria, Listeriosis, and Food Safety. CRC Press. ISBN 9781420015188. "Real-Time PCR for Cutting-Edge...
    2 KB (179 words) - 17:26, 23 April 2025
  • Thumbnail for Herpes zoster ophthalmicus
    Alternatively, fluid collected from the rash may be analyzed for VZV DNA using real-time PCR. This test is rapid, easy to perform, and is highly sensitive and specific...
    11 KB (1,020 words) - 23:34, 30 September 2024
  • Thumbnail for Toxoplasmosis
    quantitative data. Real-time PCR is useful in pathogen detection, gene expression and regulation, and allelic discrimination. This PCR technique utilizes...
    135 KB (14,984 words) - 06:02, 7 April 2025
  • Thumbnail for Salmonella
    resistance of the serotype. Newer methods of "serotyping" include xMAP and real-time PCR, two methods based on DNA sequences instead of antibody reactions. These...
    71 KB (8,225 words) - 23:37, 30 April 2025
  • Thumbnail for Microsatellite instability
    sensitivity of the real-time PCR based products without any additional analysis after the PCR amplification comparing to the traditional (PCR followed with...
    26 KB (3,130 words) - 02:23, 16 April 2025
  • Thumbnail for Mikael Kubista
    Czech Republic. Kubista has contributed to the field of quantitative real-time PCR (qPCR), with his work recognized as part of the early research in this...
    23 KB (2,031 words) - 18:37, 4 April 2025
  • during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods that...
    33 KB (4,155 words) - 13:49, 10 March 2025
  • microbial toxin itself is known. One type of PCR is real time PCR also called quantitative PCR. This type of PCR uses fluorescence and then does an analysis...
    43 KB (5,326 words) - 18:25, 7 March 2025
  • Look up real-time, realtime, or real time in Wiktionary, the free dictionary. Real-time, realtime, or real time may refer to: Real-time computing, hardware...
    4 KB (527 words) - 16:27, 18 April 2025
  • RT PCR may refer to: Real-time polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) This disambiguation page lists articles...
    151 bytes (49 words) - 19:55, 9 June 2022
  • capable real-time PCR machine. HRM analysis is performed on double stranded DNA samples. Typically the user will use polymerase chain reaction (PCR) prior...
    18 KB (2,581 words) - 13:59, 7 August 2024
  • Thumbnail for Clostridium botulinum
    Pickett DO, Cohen MN, Moore EA, et al. (January 2010). "A quadruplex real-time PCR assay for rapid detection and differentiation of the Clostridium botulinum...
    55 KB (6,003 words) - 16:09, 30 April 2025
  • studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated expression...
    17 KB (2,067 words) - 01:32, 3 December 2023
  • diagnosis based on serology. However, newer laboratory techniques such as real-time PCR and microimmunofluorescence can be used to identify Rickettsia faster...
    36 KB (3,996 words) - 16:04, 30 April 2025
  • Thumbnail for Meat and bone meal
    not-for-profit organisation. Tests are by light microscopy and using real-time PCR for ruminant DNA. The presence of MBM and fish products in feed can...
    12 KB (1,346 words) - 03:59, 16 January 2025
  • Thumbnail for COVID-19 testing
    described as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used....
    181 KB (21,007 words) - 03:07, 9 April 2025
  • RT-PCR resources- primer databases, software, protocols Archived 2010-11-25 at the Wayback Machine 2. Beacon Designer - Software to design real time PCR...
    6 KB (684 words) - 21:36, 10 May 2025
  • Thumbnail for Williams syndrome
    "Williams-Beuren syndrome: Determination of deletion size using quantitative real-time PCR". International Journal of Molecular Medicine. 18 (5): 799–806. doi:10...
    52 KB (5,792 words) - 03:17, 17 May 2025
  • Thumbnail for Chimera (genetics)
    S2CID 30548375. Faize, M.; Faize, L.; Burgos, L. (2010). "Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor...
    56 KB (6,402 words) - 14:00, 15 April 2025