Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). By adding a reverse transcriptase...

displacement amplification (SDA) are isothermal, replicating DNA at a constant temperature using a polymerase and nicking enzyme. Recombinase Polymerase Amplification...

piloted aircraft Replication protein A Recombinase polymerase amplification, isothermal alternative to the polymerase chain reaction (PCR) Robotic process...

diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method". The Journal of General Virology. 99 (8): 1012–1026...

2013). "An aptamer-based bio-barcode assay with isothermal recombinase polymerase amplification for cytochrome-c detection and anti-cancer drug screening"...

isothermal DNA amplification, e.g. in multidisplacement amplification, recombinase polymerase amplification or isothermal assembly, for the amplification of entire...

the signal from background autofluorescence. Combined with recombinase polymerase amplification, nanodiamonds enable single-copy detection of HIV-1 RNA on...

tests are under trial: Diagnostics-in-a-Suitcase, based on recombinase polymerase amplification (RPA). The new equipment, about the size of a laptop and...

isothermal amplification with technologies such as Loop-mediated isothermal amplification (LAMP) or Recombinase polymerase amplification (RPA), for example...

"Microfluidic Point-of-Care Device for Diagnosis of HIV via Recombinase Polymerase Amplification." A novel method of HIV detection in developing countries...

molecules. Recombinant DNA technology is widely used in genetic engineering. recombinase recombination See genetic recombination, homologous recombination, and...

tests that use several technologies, such as ELISA, Recombinase Polymerase Amplification (RPA), Polymerase Chain Reaction (PCR), nucleic acid hybridization...

Polymerase for transcription of the gene(s) targeted by the system. In order for a protein to be made from the gene that encodes it, RNA polymerase must...

DSB are processed into ssDNA with 3’ extensions, which allows RAD51 recombinase (eukaryotic homologue of prokaryotic RecA) to bind to it to form a nucleoprotein...

GATCs are amplified in the subsequent PCR. A PCR with primers matching the adaptors is then carried out, leading to the specific amplification of genomic...

replicating DNA sequences. Polymerase chain reaction (PCR), developed by Kary Mullis in 1983, allowed small sections of DNA to be amplified (replicated) and aided...

vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. There...

of an amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain...

by enzymatic ligation, they are amplified via rolling circle amplification using a polymerase. After the amplification reaction, several-hundredfold replication...

post-transformation excision of marker genes using phage site-specific recombinases. The Maliga team constructed the pPZP Agrobacterium binary vector family...

expression seen during stages of development in model organisms. DNA Recombinase systems like Cre-Lox recombination used in association with promoters...

S2CID 18460980. Tiscornia G, Tergaonkar V, Galimi F, Verma IM (May 2004). "CRE recombinase-inducible RNA interference mediated by lentiviral vectors". Proceedings...

3’ overhangs on the linear DNA. Subsequently, λ beta, a member of a recombinase family, binds to the 3’ overhangs and mediates annealing to the complementary...

referred to as class 1, or a 23 bp spacer, referred to as class 2. A recombinase made up of RAG1 and RAG2 subunits always cleave between these two sites...

Kumar JP (2017). "FLPing Genes on and off in Drosophila". Site-Specific Recombinases. Methods in Molecular Biology. Vol. 1642. pp. 195–209. doi:10.1007/978-1-4939-7169-5_13...