Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger...
36 KB (4,657 words) - 04:35, 10 March 2024
lifelong treatment regimen, research to find more permanent cures for HIV infection is currently underway. It is possible to synthesize zinc finger nucleotides...
28 KB (3,400 words) - 16:02, 7 April 2024
Zinc finger nucleases that disrupt the CCR5 gene in CD4+ human T-cells as a potential treatment for HIV/AIDS. The majority of engineered zinc finger arrays...
42 KB (4,825 words) - 19:40, 5 May 2024
Genome editing (redirect from Genome editing with engineered nucleases)
engineered nucleases, i.e. all three major classes of these enzymes—zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs)...
78 KB (9,362 words) - 17:58, 25 May 2024
expression through gene therapy. A procedure zinc-finger nuclease-based gene knockout has been used in a Phase I trial of 12 humans and led to an increase in CD4...
134 KB (15,824 words) - 14:29, 11 April 2024
Gene therapy (redirect from List of gene therapies for treatment of genetic diseases)
increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The vector...
172 KB (17,769 words) - 01:31, 8 May 2024
(M) or a nuclease (N). Modification of the endogenous DNA-binding zinc finger domain is the basis of the most advanced field in construction of gene-specific...
36 KB (4,966 words) - 11:20, 16 January 2024
Genetic engineering techniques (redirect from Techniques of genetic engineering)
zinc finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs)...
55 KB (6,765 words) - 09:23, 25 May 2024
Cas9 (category All Wikipedia articles in need of updating)
Alongside zinc finger nucleases and transcription activator-like effector nuclease (TALEN) proteins, Cas9 is becoming a prominent tool in the field of genome...
43 KB (5,610 words) - 21:17, 2 May 2024
CCR5 (redirect from Genetic HIV resistance)
Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728 for HIV (Zinc-Finger)". U.S. National Institutes of Health. 9 December 2009. Retrieved...
67 KB (7,844 words) - 14:24, 25 March 2024
CRISPR gene editing (section HIV/AIDS)
effector nucleases (TALENs) provided an easier way to target a double-stranded break to a specific location on the DNA strand. Both zinc finger nucleases and...
150 KB (17,439 words) - 13:58, 7 May 2024
Off-target genome editing (redirect from Off-target effects of genome editing)
effector nucleases (TALEN), meganucleases, and zinc finger nucleases (ZFN). These tools use different mechanisms to bind a predetermined sequence of DNA (“target”)...
52 KB (6,822 words) - 06:40, 1 December 2023
He Jiankui affair (redirect from DNA editing for HIV immunity)
(CD4 T cells) using zinc-finger nuclease which they introduced (infused) into 12 individuals with HIV. After complete treatment, the patients showed...
103 KB (11,553 words) - 19:09, 1 May 2024
Severe combined immunodeficiency (section Treatment)
likelihood of oncogenesis and using zinc-finger nucleases to further target gene insertion. No leukemia cases have yet been seen in trials of ADA-SCID,...
34 KB (3,258 words) - 02:42, 29 May 2024
Therapeutic gene modulation (redirect from Therapeutic regulation of gene expression)
"Highly efficient endogenous human gene correction using designed zinc-finger nucleases". Nature. 435 (7042): 646–51. Bibcode:2005Natur.435..646U. doi:10...
35 KB (3,994 words) - 17:51, 21 April 2024
Herpes (category Biology of bipolar disorder)
from monolaurin, L-lysine, aspirin, lemon balm, topical zinc, or licorice root cream in treatment, these preliminary studies have not been confirmed by...
80 KB (8,799 words) - 10:30, 29 May 2024
CRISPR (category CS1 maint: DOI inactive as of March 2024)
Glossary of genetics Human Nature (2019 documentary film) MAGESTIC Prime editing RNAi SiRNA Surveyor nuclease assay Synthetic biology Zinc finger Subtype...
153 KB (16,350 words) - 05:19, 20 May 2024
expression. Zinc-finger nucleases (ZFNs) are enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger recognizes between...
71 KB (8,212 words) - 21:58, 20 May 2024
Genetic engineering (redirect from Applications of genetic engineering)
processes of homologous recombination and nonhomologous end-joining. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription...
134 KB (14,209 words) - 09:22, 25 May 2024
Herpes simplex virus (section Treatment)
HSV-2 increases the risk of acquiring HIV. HSV has been a model virus for many studies in molecular biology. For instance, one of the first functional promoters...
68 KB (7,292 words) - 14:18, 26 May 2024
Genetically modified animal (redirect from Genetic engineering of animals)
engineered nucleases that create breaks at specific points. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription...
102 KB (11,045 words) - 15:26, 28 May 2024
Genetically modified organism (category CS1 maint: DOI inactive as of April 2024)
engineered nucleases that create breaks at specific points. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription...
222 KB (24,559 words) - 18:35, 29 May 2024
by utilizing zinc finger nucleases or by including certain sequences such as the beta-globin locus control region to direct the site of integration to...
36 KB (4,918 words) - 09:36, 27 February 2024
Polymerase chain reaction (redirect from Applications of PCR)
Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities". Applied and Environmental Microbiology...
89 KB (11,116 words) - 16:22, 29 May 2024
N (February 2005). "Recruitment of mRNA-destabilizing protein TIS11 to stress granules is mediated by its zinc finger domain". Experimental Cell Research...
184 KB (12,548 words) - 05:28, 27 April 2024
Synthetic biology (redirect from Applications of synthetic biology)
associated site-specific activity of the sTF (usually transcriptional regulation). They further used the zinc fingers as components of complex-forming sTFs, which...
150 KB (18,308 words) - 05:27, 15 April 2024
2019 in science (category Wikipedia articles in need of updating from October 2019)
Study of Genome Editing by the Zinc Finger Nuclease (ZFN) Therapeutic SB-913 in Subjects With MPS II". ClinicalTrials.gov. U.S. National Library of Medicine...
258 KB (24,775 words) - 03:09, 22 May 2024
zinc finger nuclease as well as endonuclease to prevent HSV from replicating. Most recently Jerome and his lab were able to demonstrate cleavage of latent...
83 KB (8,434 words) - 03:44, 21 December 2023