Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods that have the capability to produce substantially...
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parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993...
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High-throughput sequencing, which includes next-generation "short-read" and third-generation "long-read" sequencing methods, applies to exome sequencing, genome...
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Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Nanopore...
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electrophoresis. Recently, higher volume Sanger sequencing has been replaced by next generation sequencing methods, especially for large-scale, automated...
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Hybrid genome assembly (section Hybrid error correction and de novo assembly of single-molecule sequencing reads)
Resolving these tandem repeats can be accomplished by utilizing long third generation sequencing reads, such as those obtained using the PacBio RS DNA sequencer...
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assembled back into chromosomes using only short reads provided by next-generation- sequencing technologies (NGS), and therefore most plant genome assemblies available...
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Read (biology) (category DNA sequencing)
produced by Sanger sequencing. Beginning in the 2010s, revolutionary new technologies ushered in the Third-Generation Sequencing era (TGS). TGS is a...
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second-generation sequencing technologies. For transmission electron microscopy DNA sequencing and several other third-generation sequencing technologies...
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biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched biopolymer. Sequencing results...
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DNA sequencer (redirect from Sequencing machine)
of days. More recent, third-generation DNA sequencers such as PacBio SMRT and Oxford Nanopore offer the possibility of sequencing long molecules, compared...
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Genomics (section Early sequencing efforts)
on the technology used. Third generation sequencing technologies such as PacBio or Oxford Nanopore routinely generate sequencing reads 10-100 kb in length;...
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projects. 'The Genome and Diagnostic Centre' focusing on next and third-generation sequencing was based in the headquarters in Constance, Germany. The fully...
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History of research on Arabidopsis thaliana (section Impact of second- and third-generation sequencing technology)
Retrieved 2023-09-13. Schneeberger, Korbinian (2014). "Using next-generation sequencing to isolate mutant genes from forward genetic screens". Nature Reviews...
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spatially separated into second generation sequencing (SGS) or third generation sequencing (TGS) platforms. There is variation between these protocols,...
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Second-generation sequencing has yielded many draft genomes (close to 90% of bacterial genomes in GenBank are currently not complete); third-generation sequencing...
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new species that have never been successfully cultured. With third-generation sequencing coming to many labs, simultaneous identification of thousands...
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various next-generation sequencing platforms, there has been a substantial reduction in costs, and increase in throughput of DNA sequencing. However, the...
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Infection (section Metagenomic sequencing)
agent. This amplification step is followed by next-generation sequencing or third-generation sequencing, alignment comparisons, and taxonomic classification...
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envisioned as an advancement upon third-generation programming languages (3GL). Each of the programming language generations aims to provide a higher level...
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identification and discovery. This is enabled by the use of third-generation sequencing platforms including PacBio (Sequel I/II) by Pacific Biosciences...
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being fed by new contacts with its natural reservoir. Using third-generation sequencing technology, investigators were able to sequence samples as quickly...
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Whole genome sequencing (WGS), also known as full genome sequencing or just genome sequencing, is the process of determining the entirety of the DNA sequence...
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List of RNA-Seq bioinformatics tools (category RNA sequencing)
transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics...
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Clinical metagenomic next-generation sequencing (mNGS) is the comprehensive analysis of microbial and host genetic material (DNA or RNA) in clinical samples...
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Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry...
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PMID 22732228. Schadt EE, Turner S, Kasarskis A (2010). "A window into third-generation sequencing". Human Molecular Genetics. 19 (R2): R227 – R240. doi:10.1093/hmg/ddq416...
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David W. Deamer (section Nanopore Sequencing)
genome. Despite earlier skepticism, nanopore sequencing is now accepted as a viable third generation sequencing method. Another major area of Deamer’s research...
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profiles of some model organisms, next-generation sequencing and third-generation sequencing are the preferred techniques in metatranscriptomics. The protocol...
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Schadt, E. E.; Turner, S.; Kasarskis, A. (2010). "A window into third-generation sequencing". Human Molecular Genetics. 19 (R2): R227 – R240. doi:10.1093/hmg/ddq416...
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