• Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods that have the capability to produce substantially...
    33 KB (4,024 words) - 23:03, 6 June 2025
  • parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993...
    39 KB (4,034 words) - 13:13, 23 May 2025
  • Thumbnail for DNA sequencing
    High-throughput sequencing, which includes next-generation "short-read" and third-generation "long-read" sequencing methods, applies to exome sequencing, genome...
    136 KB (15,505 words) - 05:38, 2 June 2025
  • Thumbnail for Nanopore sequencing
    Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Nanopore...
    39 KB (4,661 words) - 14:41, 8 May 2025
  • Thumbnail for Sanger sequencing
    electrophoresis. Recently, higher volume Sanger sequencing has been replaced by next generation sequencing methods, especially for large-scale, automated...
    28 KB (3,350 words) - 19:28, 12 May 2025
  • Thumbnail for Hybrid genome assembly
    Resolving these tandem repeats can be accomplished by utilizing long third generation sequencing reads, such as those obtained using the PacBio RS DNA sequencer...
    21 KB (2,940 words) - 15:26, 8 June 2025
  • assembled back into chromosomes using only short reads provided by next-generation- sequencing technologies (NGS), and therefore most plant genome assemblies available...
    44 KB (4,099 words) - 14:04, 3 January 2025
  • Read (biology) (category DNA sequencing)
    produced by Sanger sequencing. Beginning in the 2010s, revolutionary new technologies ushered in the Third-Generation Sequencing era (TGS). TGS is a...
    16 KB (2,055 words) - 00:23, 27 June 2024
  • Thumbnail for Transmission electron microscopy DNA sequencing
    second-generation sequencing technologies. For transmission electron microscopy DNA sequencing and several other third-generation sequencing technologies...
    28 KB (3,145 words) - 04:04, 9 June 2025
  • biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched biopolymer. Sequencing results...
    13 KB (1,697 words) - 08:14, 24 September 2023
  • Thumbnail for DNA sequencer
    of days. More recent, third-generation DNA sequencers such as PacBio SMRT and Oxford Nanopore offer the possibility of sequencing long molecules, compared...
    37 KB (3,850 words) - 23:38, 23 March 2024
  • Thumbnail for Genomics
    on the technology used. Third generation sequencing technologies such as PacBio or Oxford Nanopore routinely generate sequencing reads 10-100 kb in length;...
    79 KB (7,644 words) - 14:46, 31 March 2025
  • projects. 'The Genome and Diagnostic Centre' focusing on next and third-generation sequencing was based in the headquarters in Constance, Germany. The fully...
    5 KB (455 words) - 06:13, 16 May 2024
  • Retrieved 2023-09-13. Schneeberger, Korbinian (2014). "Using next-generation sequencing to isolate mutant genes from forward genetic screens". Nature Reviews...
    83 KB (5,718 words) - 17:03, 22 May 2025
  • Thumbnail for Missense mutation
    spatially separated into second generation sequencing (SGS) or third generation sequencing (TGS) platforms. There is variation between these protocols,...
    45 KB (4,553 words) - 15:58, 22 May 2025
  • Second-generation sequencing has yielded many draft genomes (close to 90% of bacterial genomes in GenBank are currently not complete); third-generation sequencing...
    43 KB (5,495 words) - 04:08, 8 June 2025
  • Thumbnail for 16S ribosomal RNA
    new species that have never been successfully cultured. With third-generation sequencing coming to many labs, simultaneous identification of thousands...
    35 KB (3,931 words) - 15:00, 5 June 2025
  • various next-generation sequencing platforms, there has been a substantial reduction in costs, and increase in throughput of DNA sequencing. However, the...
    19 KB (2,253 words) - 04:33, 25 April 2025
  • Thumbnail for Infection
    agent. This amplification step is followed by next-generation sequencing or third-generation sequencing, alignment comparisons, and taxonomic classification...
    118 KB (12,873 words) - 15:07, 23 May 2025
  • envisioned as an advancement upon third-generation programming languages (3GL). Each of the programming language generations aims to provide a higher level...
    18 KB (1,882 words) - 04:07, 17 June 2025
  • Thumbnail for DNA barcoding
    identification and discovery. This is enabled by the use of third-generation sequencing platforms including PacBio (Sequel I/II) by Pacific Biosciences...
    100 KB (11,234 words) - 22:11, 17 June 2025
  • Thumbnail for Zaire ebolavirus
    being fed by new contacts with its natural reservoir. Using third-generation sequencing technology, investigators were able to sequence samples as quickly...
    52 KB (5,469 words) - 01:35, 16 June 2025
  • Thumbnail for Whole genome sequencing
    Whole genome sequencing (WGS), also known as full genome sequencing or just genome sequencing, is the process of determining the entirety of the DNA sequence...
    115 KB (9,584 words) - 14:04, 2 June 2025
  • List of RNA-Seq bioinformatics tools (category RNA sequencing)
    transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics...
    164 KB (20,394 words) - 21:36, 16 June 2025
  • Clinical metagenomic next-generation sequencing (mNGS) is the comprehensive analysis of microbial and host genetic material (DNA or RNA) in clinical samples...
    28 KB (3,492 words) - 21:05, 20 October 2024
  • Thumbnail for Illumina dye sequencing
    Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry...
    16 KB (2,037 words) - 22:00, 19 June 2025
  • Thumbnail for Microbiome
    PMID 22732228. Schadt EE, Turner S, Kasarskis A (2010). "A window into third-generation sequencing". Human Molecular Genetics. 19 (R2): R227 – R240. doi:10.1093/hmg/ddq416...
    134 KB (11,934 words) - 20:28, 10 June 2025
  • Thumbnail for David W. Deamer
    genome. Despite earlier skepticism, nanopore sequencing is now accepted as a viable third generation sequencing method. Another major area of Deamer’s research...
    16 KB (1,462 words) - 06:42, 25 April 2025
  • profiles of some model organisms, next-generation sequencing and third-generation sequencing are the preferred techniques in metatranscriptomics. The protocol...
    33 KB (4,188 words) - 15:44, 5 March 2024
  • Thumbnail for Eric Schadt
    Schadt, E. E.; Turner, S.; Kasarskis, A. (2010). "A window into third-generation sequencing". Human Molecular Genetics. 19 (R2): R227 – R240. doi:10.1093/hmg/ddq416...
    29 KB (3,014 words) - 21:30, 26 May 2025