isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. DNA extraction is the process of isolating DNA from the cells of an organism...
25 KB (3,220 words) - 21:41, 23 May 2025
Plasmid preparation (redirect from Dna preparation)
A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and...
18 KB (2,038 words) - 07:32, 7 January 2025
Electroelution (section DNA extraction)
the surface for extraction and subsequent analysis. For example, electroblotting is based upon the same principle. Using this method, DNA fragments can...
3 KB (299 words) - 12:48, 25 April 2025
leaving only DNA. The most common methods of DNA extraction include organic extraction (also called phenol–chloroform extraction), Chelex extraction, and solid-phase...
104 KB (11,520 words) - 00:57, 29 May 2025
Differential extraction (also known as differential lysis) refers to the process by which the DNA from two different types of cells can be extracted without...
6 KB (767 words) - 06:33, 26 April 2025
Plasmid (redirect from DNA plasmids)
plasmid DNA. Many commercial kits have been created to perform plasmid extraction at various scales, purity, and levels of automation. Plasmid DNA may appear...
69 KB (7,776 words) - 22:27, 24 May 2025
Cetrimonium bromide (section DNA extraction)
fungi. It is also one of the main components of some buffers for the extraction of DNA. It has been widely used in synthesis of gold nanoparticles (e.g....
21 KB (2,455 words) - 12:43, 10 February 2025
Body identification (section DNA extraction)
DNA identification are menstrual blood, venous blood, semen, saliva and vaginal secretion. The process of DNA profiling includes DNA extraction, DNA quantification...
26 KB (3,038 words) - 17:23, 23 May 2025
Archaeogenetics (category Ancient DNA (human))
contains six times the DNA when compared to stored bones. The temperature of extraction site also affects the amount of obtainable DNA, evident by a decrease...
47 KB (5,815 words) - 01:06, 26 May 2025
detection of rare tumour-derived DNA (ctDNA) from blood samples. DNA extraction Ethanol precipitation Phenol-chloroform extraction Spin column-based purification...
14 KB (2,152 words) - 00:40, 11 March 2023
Ethanol precipitation (section DNA precipitation)
RNA, DNA, and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding salt and ethanol as an antisolvent. In DNA extraction, after...
9 KB (1,106 words) - 01:47, 14 May 2025
Forensic dentistry (section DNA extraction)
determinations by using radiographs, ante- and post-mortem photographs, and DNA analysis. Another type of evidence that may be analyzed is bite marks, whether...
71 KB (8,992 words) - 17:10, 23 May 2025
Homo floresiensis (section DNA extraction attempt)
has higher concentrations of DNA. Moreover, the heat generated by the high speed of the drill bit may have denatured the DNA. The small brain size of H...
73 KB (7,624 words) - 19:57, 22 May 2025
Library (biology) (redirect from DNA library)
different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and randomized...
11 KB (1,204 words) - 21:21, 22 April 2024
of purifying their components, as in protein purification, DNA extraction, RNA extraction, or in purifying organelles. Many species of bacteria are subject...
11 KB (1,111 words) - 02:15, 16 January 2025
DNA fragmentation is the separation or breaking of DNA strands into pieces. It can be done intentionally by laboratory personnel or by cells, or can occur...
12 KB (1,498 words) - 21:43, 24 May 2025
thiocyanate-phenol-chloroform extraction. Usually, the phenol-chloroform solution used for RNA extraction has lower pH, this aids in separating DNA from RNA and leads...
7 KB (787 words) - 09:57, 9 April 2025
In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose...
4 KB (554 words) - 04:19, 12 May 2024
Protection of Underground Networks samples soil and extracts and sequences fungal DNA in order to learn which fungi are present. The geo-located fungal taxa are...
15 KB (1,323 words) - 16:59, 20 April 2025
or blood from e.g. leeches. DNA barcoding requires that DNA in the sample is extracted. Several different DNA extraction methods exist, and factors like...
99 KB (11,132 words) - 12:01, 22 May 2025
protonates the iminodiacetate groups. Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including...
2 KB (283 words) - 14:04, 28 March 2023
any DNA sequencing protocol relies upon the DNA or RNA sample extraction and preparation from the biological material of interest. A successful DNA extraction...
136 KB (15,496 words) - 08:19, 29 May 2025
specifically for each type of extraction. For DNA extraction, the pH is adjusted to 7.0–8.0. For RNA-specific extraction, the pH is adjusted to 4.5. At...
4 KB (486 words) - 18:19, 27 December 2024
Surveyor nuclease assay (section DNA Extraction)
Initially, the DNA of interest (nuclear or mitochondrial DNA) is extracted from tissues or cell culture. This can be done by standard extraction methods such...
26 KB (3,176 words) - 03:46, 22 January 2024
mammoth. Methods have been developed to purify DNA from organisms, such as phenol-chloroform extraction, and to manipulate it in the laboratory, such as...
167 KB (17,915 words) - 22:22, 29 May 2025
Dire wolf (section DNA evidence)
from the La Brea pits, hindering the extraction of DNA samples. In 2021, researchers sequenced the nuclear DNA (from the cell nucleus) taken from five...
126 KB (13,450 words) - 12:09, 28 May 2025
Boom method (redirect from Magnetic DNA Extraction)
Boom method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method...
22 KB (2,552 words) - 16:20, 8 June 2024
ancient DNA analysis. The success rate of usable DNA extraction is relatively high in paleofeces, making it more reliable than skeletal DNA retrieval...
10 KB (1,255 words) - 00:51, 23 January 2025
Haplogroup R1b (redirect from Haplogroup R1b1c (Y-DNA))
"Kinship and Y-chromosome analysis of 7th century human remains: novel DNA extraction and typing procedure for ancient material". Croatian Medical Journal...
89 KB (8,412 words) - 15:57, 10 May 2025
Entamoeba spp. The DNA-based diagnostic tests include the use of DNA extraction, PCR, microarrays, and typing methods.For example, one DNA-based diagnostic...
13 KB (1,517 words) - 22:26, 4 May 2025