Cell-free protein array technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This...

M; Taussig, MJ (June 2001). "Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)". Nucleic...

Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions...

produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals. HEK 293 cells were generated in 1973...

interactions. It is a two-dimensional array on a solid substrate—usually a glass slide or silicon thin-film cell—that assays (tests) large amounts of biological...

protein N- and O-linked glycans from mammalian cells, with highest priority given to pure populations of human or murine immune cells. Glycan Array Synthesis...

Raymond GJ, Lansbury PT, et al. (August 1994). "Cell-free formation of protease-resistant prion protein". Nature. 370 (6489): 471–4. Bibcode:1994Natur...

Glycan–protein interactions represent a class of biomolecular interactions that occur between free or protein-bound glycans and their cognate binding partners...

functions are largely unknown. Tiling arrays aid in transcriptome mapping as well as in discovering sites of DNA/protein interaction (ChIP-chip, DamID), of...

concentrations of free calcium in the cell can regulate an array of reactions and is important for signal transduction in the cell. Mitochondria can transiently...

bacteria. This leads to signal transduction to a G protein and activation of the mast cell. Mast cell activation induces the release of antibacterial mediators...

Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function...

failure in multiple myeloma is due to proteins secreted by the malignant cells. Myeloma cells produce monoclonal proteins of varying types, most commonly immunoglobulins...

or secretory form of a protein. This is required, for instance, to protect the secretory cell from the activity of that protein. Apoenzymes become active...

Reddy S, Moody SA, Nemes P (August 2016). "Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus...

transmembrane III isoform, Schwann cell precursors are eventually eliminated from spinal nerves. Myelin protein zero (P0) is a cell-adhesion molecule belonging...

responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including...

nucleic acid sequences in the target. The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based...

outer surface of the cell membrane by a glycosylphosphatidylinositol anchor at its C-terminal Ser231.[citation needed] Prion protein contains five octapeptide...

axis of the muscle cells in subsarcolemmal locations, free myofilaments become aligned and aggregate into hexagonally packed arrays. These aggregates form...

certain. Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined...

Microfilaments, also called actin filaments, are protein filaments in the cytoplasm of eukaryotic cells that form part of the cytoskeleton. They are primarily...

then is free to fold. The ATPase cycle can be synergistically enhanced by protein disulfide isomerase (PDI), and its cochaperones. When K12 cells are starved...

receptors are found in plant cells. Integrins work alongside other proteins such as cadherins, the immunoglobulin superfamily cell adhesion molecules, selectins...

a genetic mutation coding for a structural membrane protein phenotype causes the red blood cells to be sphere-shaped (spherocytosis), rather than the...

and immunocytochemistry, where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA)...

has been found to encompass a wide variety of differing protein structures in eukaryotic cells. Xenopus laevis TFIIIA was originally demonstrated to contain...

cytometry include: Cell counting Cell sorting Determining cell characteristics and function Detecting microorganisms Biomarker detection Protein engineering...

unrelated proteins, but both are normally expressed in the cells of breast and other tissues, where they help repair damaged DNA, or destroy cells if DNA...

such arrays are perfectly suited to study gene expression profiles, they have limited importance in ChIP experiments since most "interesting" proteins with...