A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition...
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In a restriction digest, DNA molecules are cleaved at specific restriction sites of 4-12 nucleotides in length by use of restriction enzymes which recognize...
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restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction...
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polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences...
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sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome. Once RAD tags have been isolated...
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Restriction enzyme mediated integration (abbreviated as REMI) is a technique for integrating DNA (linearised plasmid) into the genome sites that have been...
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Endonuclease (redirect from Endonuclease enzyme)
regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences...
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piece using Type IIS restriction enzymes and T4 DNA ligase. This assembly is performed in vitro. Most commonly used Type IIS enzymes include BsaI, BsmBI...
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such as that borne by bacteriophages. Bacteria have restriction enzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific...
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A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria...
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list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of...
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Thermus aquaticus (section Taq I restriction enzyme)
Thermus aquaticus arose at this time from the convention of giving restriction enzymes short names, such as Sal and Hin, derived from the genus and species...
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A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called...
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is designing complementary oligonucleotide sequences that contain restriction enzyme sites along with additional bases on the end that are complementary...
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Palindromic sequence (section Restriction enzyme sites)
palindrome. Many restriction endonucleases (restriction enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes...
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of the two types of enzymes responsible for phage growth restriction in Escherichia coli (E. coli) bacteria. One of these enzymes added a methyl group...
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EcoRI (category Restriction enzymes)
(pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices...
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DNA shuffling (section Restriction enzymes)
recombination or the similarity of the DNA sequences, restriction enzymes which rely on common restriction sites, and nonhomologous random recombination which...
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target DNA, and then treated with a restriction enzyme. If the probe exactly matches the target, the restriction enzyme will cleave the probe, changing its...
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at a specific recognition nucleotide sequence known as the restriction site. Such enzymes hydrolyze (cut) only one strand of the DNA duplex, to produce...
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by restriction enzymes that break the molecule's phosphodiester backbone at specific locations, which themselves belong to a larger class of enzymes called...
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purified by size fractionation. Using a second enzyme, DNA ligase, fragments generated by restriction enzymes could be joined in new combinations, termed...
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declarations Restriction enzyme, a type of enzyme that cleaves genetic material Restriction, a term in medieval supposition theory Restriction (mathematics)...
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from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by electrophoresis...
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FokI (redirect from FokI restriction endonuclease)
The restriction endonuclease Fok1, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal...
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DpnI (category Restriction enzymes)
interactions. DpnII restriction endonuclease family, also isolated from S. pneumonae List of restriction enzyme cutting sites Restriction modification system...
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A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular...
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HindIII (category Restriction enzymes)
(pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic...
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visualization of thousands of landmarks, or restriction sites. Using a combination of restriction enzymes some of which are specific to DNA modifications...
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Zurich. In 1970, Smith and Kent W. Wilcox discovered the first type II restriction enzyme, which is now known as HindII. Smith went on to discover DNA methylases...
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