A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites—a standard feature...

been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types of cloning vectors...

molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector). The vector typically contains a multiple cloning site where...

vectors used in DNA constructs contain an origin of replication, a multiple cloning site, and a selectable marker. Certain vectors can carry additional regulatory...

cassette is part of a pre-constructed vector, usually containing a multiple cloning site (MCS) upstream of the cassette. For this reason, promoters of interest...

Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments...

marker (CmR in this case), origin of replication, araC and operons, multiple cloning site and PBAD promoter. Studies show that vectors are highly expressed...

vectors may be used for cloning and are therefore cloning vectors, but there are also vectors designed specially for cloning, while others may be designed...

asymmetric recognition sites; this characteristic is widely used to perform in-vitro cloning techniques such as Golden Gate cloning. These enzymes may function...

bacterial cloning vectors. These cloning vectors contain a site that allows DNA fragments to be inserted, for example a multiple cloning site or polylinker...

connects two nucleosomes Polylinker or multiple cloning site, a short segment of DNA with many restriction sites Signal transducing adaptor protein, proteins...

commonly used chemicals Multiple cloning site, the region of restriction enzyme sites in a plasmid Master of Computer Science Multiple Console Support, in...

like most other plasmids, include a selectable marker and an MCS (multiple cloning site) located at the end of the GFP gene. The plasmid is 5371 base pairs...

coli, also referred to as the α-peptide. The multiple cloning site (MCS), which contains many restriction sites, is split into codons 6-7 of the lacZ gene...

transformation of eubacteria. It is 5,437 base pairs long. There is a multiple cloning site in the lacZ gene. "BIOTECHNOLOGY: BACTERIAL TRANSFORMATION" (PDF)...

DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine. In a conventional molecular cloning experiment, the...

selection during cloning multiple cloning site derived from pBluescript. pGreenII 62-SK: derived from pGreenII 0000, the LacZ blue/white cloning selection has...

cells resulted in the characteristic blue colonies. However, the multiple cloning site, where a gene of interest may be ligated into the plasmid vector...

The Gateway cloning method is a method of molecular cloning invented and commercialized by Invitrogen since the late 1990s, which makes use of the integration...

replication, a multiple cloning site, and a selectable marker. Vectors are widely used in molecular biology laboratories to isolate, clone, or express the...

"Foundations of Molecular Cloning - Past, Present and Future | NEB". www.neb.com. Retrieved 2021-11-25. "Foundations of Molecular Cloning - Past, Present and...

a suitable site for the insertion of a gene like the multiple cloning site. The cloned gene may be transferred from a specialized cloning vector to an...

process. The plasmid carries within the lacZα sequence an internal multiple cloning site (MCS). This MCS within the lacZα sequence can be cut by restriction...

Multiple sclerosis (MS) is an autoimmune disease resulting in damage to myelin – the insulating covers of nerve cells – in the brain and spinal cord. As...

containing X-gal, forming characteristic blue colonies. However, the multiple cloning site, where a gene of interest may be ligated into the plasmid vector...

model - mRNA - multicistronic message - multicopy plasmid - multiple cloning site - multiple endocrine neoplasia, type 1 - mutation - myristoyl-CoA 11-(E)...

β-galactosidase) is also used to screen for blue white colonies in the multiple cloning sites of various plasmid vectors in Escherichia coli or other bacteria...

encoding either the minor or major coat protein, respectively. Multiple cloning sites are sometimes used to ensure that the fragments are inserted in...

Multiple myeloma (MM), also known as plasma cell myeloma and simply myeloma, is a cancer of plasma cells, a type of white blood cell that normally produces...

Circular Polymerase Extension Cloning (CPEC), Sequence and Ligase Independent Cloning (SLIC) and Seamless Ligation Cloning Extract (SLiCE). Despite the...