Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this...
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Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing...
76 KB (8,040 words) - 07:21, 2 November 2024
Cytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count...
15 KB (1,679 words) - 17:49, 30 January 2024
Photoacoustic flow cytometry or PAFC is a biomedical imaging modality that utilizes photoacoustic imaging to perform flow cytometry. A flow of cells passes...
21 KB (2,889 words) - 14:25, 6 July 2024
suspicion for CLL, and a confirmatory diagnostic test, in particular flow cytometry should be performed unless clinically unnecessary. In order to be diagnosed...
76 KB (8,154 words) - 19:15, 21 May 2025
Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments. The FCS specification has traditionally...
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stream and their properties are measured as they flow past sensors in a technique known as flow cytometry. Hydrodynamic focusing may be used to isolate individual...
106 KB (12,446 words) - 06:39, 6 April 2025
during flow cytometry. Manual gating of this data can be performed as is done for flow cytometry and most of the tools available for flow cytometry analysis...
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corrected electronically. The compensation can be done through different flow cytometry software such as Flowjo, Flowlogic, Kaluza etc. The first data compensation...
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Daratumumab (section With flow cytometry testing)
notified when sending such a sample. Daratumumab can also interfere with flow cytometric evaluation of multiple myeloma, causing an apparent lack of plasma...
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populations via the combination of flow cytometry with cytogenetic fluorescent in situ hybridization staining protocols. Flow-FISH is most commonly used to...
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Anti-dsDNA antibodies (section Flow cytometry)
detection. The beads are run through a flow cell which uses a laser to detect fluorescence. Similar to the flow cytometry method of ANA detection, the MIA uses...
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include techniques of sample preparation, cell separation, cell sorting, flow cytometry, spatial biology, cell culture, molecular analysis, clinical applications...
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Virus quantification (section Flow cytometry)
concentration. While most flow cytometers do not have sufficient sensitivity,[citation needed] there are a few commercially available flow cytometers that can...
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microscopy and immunohistochemistry, often with other fluorophores Flow cytometry to count or sort out cells. An example is the use of Hoechst dyes to...
12 KB (1,285 words) - 21:31, 5 January 2024
Cell counting (section Flow cytometry)
significance. Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a narrow stream...
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to UV light during flow cytometry, X spermatozoa fluoresce brighter than Y- spermatozoa. As the spermatozoa pass through the flow cytometer in single...
47 KB (5,656 words) - 16:45, 4 May 2025
production of a stable ENaC cell line using fluorogenic signaling probes and flow cytometry to scan numerous cells to isolate rare clones capable of functional...
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positive control when assessing the acrosome reaction of a sperm sample by flow cytometry or fluorescence microscopy. This is usually done after staining with...
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scatter. To summarize, flow cytometry alone can be used to gather quantitative data about cell cycle phase distribution, but flow cytometry in coordination with...
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Consortium The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) is a standard related to flow cytometry which establishes criteria to record...
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differential counts: digital image processing of microscope slides and flow cytometry techniques using light scattering and cell staining. These methods remain...
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width Platelet mean volume Large platelet cell ratio Platelet criteria Flow cytometry Spectrophotometry Absorption spectroscopy Transmission Turbidimetry...
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Immunostaining (category Flow cytometry)
used for immunofluorescence, and then analysed by flow cytometry.[citation needed] Flow cytometry has several advantages over IHC including: the ability...
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which bind to the native (non-denaturated) forms of antigen proteins. Flow cytometry screening has been used for primary screening of a large number (~1000)...
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multiparameter flow- and laser scanning- cytometry. It has been used to describe the real-time changes during cell death, detected by flow cytometry. Programmed...
21 KB (2,738 words) - 13:17, 23 April 2025
microscopy, flow cytometry or fluorescence microscopy can be used for assessing the shedding of the acrosome or "acrosome reaction" of a sperm sample. Flow cytometry...
18 KB (2,388 words) - 16:55, 17 May 2025
between T cells and B cells in a peripheral blood smear. Normally, flow cytometry testing is used for specific lymphocyte population counts. This can...
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FlowJo is a software package for analyzing flow cytometry data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard format...
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Tetramer assay (category Flow cytometry)
tetramer-bound T-cells to be analyzed with flow cytometry. Quantification and sorting of T-cells by flow cytometry enables researchers to investigate immune...
13 KB (1,492 words) - 15:49, 7 June 2024