• molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of proteinprotein interactions. In...
    12 KB (1,325 words) - 16:36, 22 July 2025
  • Thumbnail for Bimolecular fluorescence complementation
    Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association...
    46 KB (5,192 words) - 21:42, 24 June 2025
  • Thumbnail for Two-hybrid screening
    Two-hybrid screening (category Proteinprotein interaction assays)
    protein-fragment complementation assay. Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein...
    49 KB (6,276 words) - 14:29, 22 July 2025
  • Protein-fragment complementation assay Co-immunoprecipitation Affinity purification and mass spectrometry Proximity ligation assay Proximity labeling...
    20 KB (2,286 words) - 02:41, 30 June 2025
  • Thumbnail for Protein–protein interaction
    transfer systems, mammalian proteinprotein interaction trap, electro-switchable biosurfaces, proteinfragment complementation assay, as well as real-time label-free...
    74 KB (8,383 words) - 11:23, 12 July 2025
  • atrophy, a form of dementia Prostate cancer Protein-fragment complementation assay, to identify proteinprotein interactions Protocatechuic acid, a phenolic...
    4 KB (418 words) - 15:21, 27 May 2025
  • Thumbnail for Protein structure
    in a variety of organisms based on intragenic complementation evidence was reviewed in 1965. Proteins are frequently described as consisting of several...
    38 KB (4,246 words) - 18:23, 16 July 2025
  • pairs of proteins can be inferred from a variety of techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity...
    25 KB (2,915 words) - 05:18, 2 June 2025
  • PFCA may refer to: Protein-fragment complementation assay Perfluorinated carboxylic acid The Planar Fourier Capture Array, a tiny camera built entirely...
    263 bytes (55 words) - 18:27, 29 December 2019
  • Thumbnail for Reporter gene
    used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. Reporter genes can produce a protein that has...
    37 KB (3,978 words) - 14:16, 22 July 2025
  • Thumbnail for Dihydrofolate reductase
    Dihydrofolate reductase (category Protein domains)
    used as a tool to detect proteinprotein interactions in a protein-fragment complementation assay (PCA), using a split-protein approach. DHFR-lacking CHO...
    41 KB (4,586 words) - 08:15, 26 July 2025
  • Thumbnail for Proteome
    mixture of proteins. Protein-fragment complementation assays are often used to detect proteinprotein interactions. The yeast two-hybrid assay is the most...
    26 KB (3,013 words) - 14:07, 18 July 2025
  • Thumbnail for Complement component 2
    Complement C2 is a protein that in humans is encoded by the C2 gene. The protein encoded by this gene is part of the classical pathway of the complement...
    16 KB (2,068 words) - 16:58, 16 July 2025
  • Thumbnail for Protein folding
    MM, Rüdiger SG (2012). "Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...
    76 KB (8,712 words) - 18:23, 16 July 2025
  • Thumbnail for Green fluorescent protein
    signaling molecules, such as calcium or glutamate, protein phosphorylation state, protein complementation, receptor dimerization, and other processes provide...
    66 KB (7,627 words) - 10:56, 14 July 2025
  • Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system...
    35 KB (4,189 words) - 16:09, 17 July 2025
  • of complement (serum). Then cell death is determined with several approaches: Radioactive method: target cells are labeled with 51Cr before CDC assay, chromium...
    8 KB (957 words) - 03:12, 2 June 2025
  • Thumbnail for FANCA
    gene. It belongs to the Fanconi anaemia complementation group (FANC) family of genes of which 12 complementation groups are currently recognized and is...
    38 KB (4,761 words) - 17:18, 16 July 2025
  • Thumbnail for Β-Galactosidase
    Β-Galactosidase (category Protein pages needing a picture)
    amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. Its residues 22–31 help to stabilize...
    26 KB (3,045 words) - 13:05, 17 July 2025
  • Thumbnail for Antibody
    Antibody (redirect from Fragment variable)
    rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection...
    126 KB (13,978 words) - 20:50, 16 July 2025
  • Thumbnail for X-gal
    the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ...
    11 KB (1,070 words) - 18:14, 26 July 2025
  • Thumbnail for Respiratory syncytial virus
    These include both enzyme immunosorbent assays (EIA) and chromatographic immunoassays (CIA). Direct fluorescence assay (DFA) allows for direct microscopic...
    98 KB (9,646 words) - 23:17, 26 July 2025
  • performing a digestion on a genomic sample and determining fragment lengths through a gel assay it is possible to ascertain whether or not the enzymes cut...
    37 KB (5,016 words) - 17:36, 22 July 2025
  • Thumbnail for Epstein–Barr virus
    PMID 33827095. Zhang Q, Chen Y (2022). "Accelerated strand exchange amplification assay for rapid detection of Epstein–Barr virus in saliva". Analytical Biochemistry...
    66 KB (6,850 words) - 02:52, 24 July 2025
  • Thumbnail for Blue–white screen
    method is based on the principle of α-complementation of the β-galactosidase gene. This phenomenon of α-complementation was first demonstrated in work done...
    13 KB (1,821 words) - 00:36, 3 December 2023
  • Eastern blot (category Protein methods)
    et al. (2006). "A novel antibody-free method for protein blotting using enzyme fragment complementation". BioTechniques. 40 (3): 381–383. doi:10.2144/000112119...
    18 KB (2,108 words) - 14:48, 19 June 2025
  • Thumbnail for Molecular biology
    accurate protein quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not...
    50 KB (5,675 words) - 18:54, 23 July 2025
  • Thumbnail for Thrombin
    Thrombin (category Acute-phase proteins)
    membrane protein expressed by endothelial cells. Activated protein C inactivates factors Va and VIIIa. Binding of activated protein C to protein S leads...
    25 KB (2,932 words) - 18:47, 16 July 2025
  • Thumbnail for Pepsin
    Pepsin (category Protein pages needing a picture)
    F(ab')2 fragment enables it to cross-link antigens, allowing use for precipitation assays, cellular aggregation via surface antigens, or rosetting assays. The...
    30 KB (3,451 words) - 04:25, 5 May 2025
  • Thumbnail for Proteomics
    Proteomics (redirect from Protein analysis)
    kinetic exclusion assay, and experimental methods such as phage display and in silico computational methods. Knowledge of protein-protein interactions is...
    78 KB (9,072 words) - 15:13, 24 June 2025