molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions. In...

Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association...

transfer systems, mammalian protein–protein interaction trap, electro-switchable biosurfaces, protein–fragment complementation assay, as well as real-time label-free...

protein-fragment complementation assay. Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein...

Protein-fragment complementation assay Co-immunoprecipitation Affinity purification and mass spectrometry Proximity ligation assay Proximity labeling...

atrophy, a form of dementia Prostate cancer Protein-fragment complementation assay, to identify protein–protein interactions Protocatechuic acid, a phenolic...

in a variety of organisms based on intragenic complementation evidence was reviewed in 1965. Proteins are frequently described as consisting of several...

pairs of proteins can be inferred from a variety of techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity...

used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. Reporter genes can produce a protein that has...

PFCA may refer to: Protein-fragment complementation assay Perfluorinated carboxylic acid The Planar Fourier Capture Array, a tiny camera built entirely...

Complement C2 is a protein that in humans is encoded by the C2 gene. The protein encoded by this gene is part of the classical pathway of the complement...

used as a tool to detect protein–protein interactions in a protein-fragment complementation assay (PCA), using a split-protein approach. DHFR-lacking CHO...

mixture of proteins. Protein-fragment complementation assays are often used to detect protein–protein interactions. The yeast two-hybrid assay is the most...

MM, Rüdiger SG (2012). "Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...

signaling molecules, such as calcium or glutamate, protein phosphorylation state, protein complementation, receptor dimerization, and other processes provide...

Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system...

of complement (serum). Then cell death is determined with several approaches: Radioactive method: target cells are labeled with 51Cr before CDC assay, chromium...

the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ...

rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection...

amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. Its residues 22–31 help to stabilize...

et al. (2006). "A novel antibody-free method for protein blotting using enzyme fragment complementation". BioTechniques. 40 (3): 381–383. doi:10.2144/000112119...

gene. It belongs to the Fanconi anaemia complementation group (FANC) family of genes of which 12 complementation groups are currently recognized and is...

performing a digestion on a genomic sample and determining fragment lengths through a gel assay it is possible to ascertain whether or not the enzymes cut...

These include both enzyme immunosorbent assays (EIA) and chromatographic immunoassays (CIA). Direct fluorescence assay (DFA) allows for direct microscopic...

PMID 33827095. Zhang Q, Chen Y (2022). "Accelerated strand exchange amplification assay for rapid detection of Epstein–Barr virus in saliva". Analytical Biochemistry...

method is based on the principle of α-complementation of the β-galactosidase gene. This phenomenon of α-complementation was first demonstrated in work done...

accurate protein quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not...

membrane protein expressed by endothelial cells. Activated protein C inactivates factors Va and VIIIa. Binding of activated protein C to protein S leads...

kinetic exclusion assay, and experimental methods such as phage display and in silico computational methods. Knowledge of protein-protein interactions is...

Some segments of DNA are transcribed into RNA molecules that can encode proteins, called messenger RNA (mRNA). Other segments of DNA are transcribed into...