molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions. In...
12 KB (1,325 words) - 16:36, 22 July 2025
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association...
46 KB (5,192 words) - 21:42, 24 June 2025
Two-hybrid screening (category Protein–protein interaction assays)
protein-fragment complementation assay. Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein...
49 KB (6,276 words) - 14:29, 22 July 2025
Protein-fragment complementation assay Co-immunoprecipitation Affinity purification and mass spectrometry Proximity ligation assay Proximity labeling...
20 KB (2,286 words) - 02:41, 30 June 2025
transfer systems, mammalian protein–protein interaction trap, electro-switchable biosurfaces, protein–fragment complementation assay, as well as real-time label-free...
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atrophy, a form of dementia Prostate cancer Protein-fragment complementation assay, to identify protein–protein interactions Protocatechuic acid, a phenolic...
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in a variety of organisms based on intragenic complementation evidence was reviewed in 1965. Proteins are frequently described as consisting of several...
38 KB (4,246 words) - 18:23, 16 July 2025
pairs of proteins can be inferred from a variety of techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity...
25 KB (2,915 words) - 05:18, 2 June 2025
PFCA may refer to: Protein-fragment complementation assay Perfluorinated carboxylic acid The Planar Fourier Capture Array, a tiny camera built entirely...
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Reporter gene (redirect from Reporter gene assay)
used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. Reporter genes can produce a protein that has...
37 KB (3,978 words) - 14:16, 22 July 2025
Dihydrofolate reductase (category Protein domains)
used as a tool to detect protein–protein interactions in a protein-fragment complementation assay (PCA), using a split-protein approach. DHFR-lacking CHO...
41 KB (4,586 words) - 08:15, 26 July 2025
mixture of proteins. Protein-fragment complementation assays are often used to detect protein–protein interactions. The yeast two-hybrid assay is the most...
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Complement C2 is a protein that in humans is encoded by the C2 gene. The protein encoded by this gene is part of the classical pathway of the complement...
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MM, Rüdiger SG (2012). "Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...
76 KB (8,712 words) - 18:23, 16 July 2025
signaling molecules, such as calcium or glutamate, protein phosphorylation state, protein complementation, receptor dimerization, and other processes provide...
66 KB (7,627 words) - 10:56, 14 July 2025
Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system...
35 KB (4,189 words) - 16:09, 17 July 2025
of complement (serum). Then cell death is determined with several approaches: Radioactive method: target cells are labeled with 51Cr before CDC assay, chromium...
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FANCA (redirect from Fanconi anemia, complementation group A)
gene. It belongs to the Fanconi anaemia complementation group (FANC) family of genes of which 12 complementation groups are currently recognized and is...
38 KB (4,761 words) - 17:18, 16 July 2025
Β-Galactosidase (category Protein pages needing a picture)
amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. Its residues 22–31 help to stabilize...
26 KB (3,045 words) - 13:05, 17 July 2025
Antibody (redirect from Fragment variable)
rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection...
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X-gal (section Protein-protein interactions)
the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ...
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Respiratory syncytial virus (section G protein)
These include both enzyme immunosorbent assays (EIA) and chromatographic immunoassays (CIA). Direct fluorescence assay (DFA) allows for direct microscopic...
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SNP genotyping (redirect from Oligo Pool Assay)
performing a digestion on a genomic sample and determining fragment lengths through a gel assay it is possible to ascertain whether or not the enzymes cut...
37 KB (5,016 words) - 17:36, 22 July 2025
Epstein–Barr virus (section Protein/genes)
PMID 33827095. Zhang Q, Chen Y (2022). "Accelerated strand exchange amplification assay for rapid detection of Epstein–Barr virus in saliva". Analytical Biochemistry...
66 KB (6,850 words) - 02:52, 24 July 2025
method is based on the principle of α-complementation of the β-galactosidase gene. This phenomenon of α-complementation was first demonstrated in work done...
13 KB (1,821 words) - 00:36, 3 December 2023
Eastern blot (category Protein methods)
et al. (2006). "A novel antibody-free method for protein blotting using enzyme fragment complementation". BioTechniques. 40 (3): 381–383. doi:10.2144/000112119...
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Molecular biology (section The Bradford protein assay)
accurate protein quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not...
50 KB (5,675 words) - 18:54, 23 July 2025
Thrombin (category Acute-phase proteins)
membrane protein expressed by endothelial cells. Activated protein C inactivates factors Va and VIIIa. Binding of activated protein C to protein S leads...
25 KB (2,932 words) - 18:47, 16 July 2025
Pepsin (category Protein pages needing a picture)
F(ab')2 fragment enables it to cross-link antigens, allowing use for precipitation assays, cellular aggregation via surface antigens, or rosetting assays. The...
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Proteomics (redirect from Protein analysis)
kinetic exclusion assay, and experimental methods such as phage display and in silico computational methods. Knowledge of protein-protein interactions is...
78 KB (9,072 words) - 15:13, 24 June 2025