• RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in...
    7 KB (787 words) - 09:57, 9 April 2025
  • Thumbnail for Trizol
    TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by...
    5 KB (459 words) - 23:09, 12 November 2024
  • Thumbnail for Ribonuclease
    abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases...
    20 KB (2,022 words) - 18:38, 29 April 2025
  • of purifying their components, as in protein purification, DNA extraction, RNA extraction, or in purifying organelles. Many species of bacteria are subject...
    11 KB (1,111 words) - 02:15, 16 January 2025
  • be produced. Isolated mRNA should range from 500 bp to 8 kb. Several methods exist for purifying RNA such as trizol extraction and column purification...
    8 KB (941 words) - 23:02, 25 May 2025
  • deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. DNA extraction is the process of isolating DNA from the cells of an organism isolated...
    25 KB (3,236 words) - 23:11, 23 June 2025
  • Thumbnail for Acid guanidinium thiocyanate-phenol-chloroform extraction
    extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology. It is widely used for isolating RNA (as...
    6 KB (785 words) - 10:52, 25 January 2025
  • Thumbnail for Complementary DNA
    column, and bead-based RNA extraction methods. Extraction methods vary depending on the source material. For example, extracting RNA from plant tissue requires...
    19 KB (2,275 words) - 04:33, 26 May 2025
  • Thumbnail for RNA world
    The RNA world is a hypothetical stage in the evolutionary history of life on Earth in which self-replicating RNA molecules proliferated before the evolution...
    96 KB (11,196 words) - 19:59, 18 June 2025
  • Thumbnail for DNA sequencing
    a DNA sample with long, non-degraded strands. A successful RNA extraction will yield a RNA sample that should be converted to complementary DNA (cDNA)...
    136 KB (15,505 words) - 05:38, 2 June 2025
  • Phenol–chloroform extraction is a liquid-liquid extraction technique in molecular biology used to separate nucleic acids from proteins and lipids. Aqueous...
    3 KB (393 words) - 00:37, 8 January 2024
  • Thumbnail for Polyvinylpyrrolidone
    capsules) and as an inhibitor of recrystallisation as an additive to Doro's RNA extraction buffer [citation needed] as a liquid-phase dispersion enhancing agent...
    20 KB (2,124 words) - 11:12, 15 June 2025
  • transcripts. Small RNAs, such as micro RNAs, can be purified based on their size by gel electrophoresis and extraction. Since mRNAs are longer than the...
    120 KB (12,519 words) - 13:36, 25 January 2025
  • manufacturing. Common applications include: Cell disruption for DNA, RNA, and protein extraction Food and beverage production (e.g., milk, juices, sauces) Cosmetic...
    6 KB (712 words) - 16:18, 21 May 2025
  • specifically for each type of extraction. For DNA extraction, the pH is adjusted to 7.0–8.0. For RNA-specific extraction, the pH is adjusted to 4.5. At...
    4 KB (486 words) - 18:19, 27 December 2024
  • Total RNA Miniprep Kit was not designed specifically for viral RNA extraction, but it was successfully used by different companies to extract viral RNA from...
    23 KB (2,213 words) - 00:34, 25 May 2025
  • problem was resolved by adding RNA of a distantly related Drosophila species to each tube after initial RNA extraction. NanoString's GeoMx Digital Spatial...
    64 KB (7,251 words) - 21:48, 23 June 2025
  • to study nucleic acids: DNA and RNA. DNA extraction Phenol–chloroform extraction Minicolumn purification RNA extraction Boom method Synchronous coefficient...
    4 KB (378 words) - 01:27, 24 June 2025
  • sample harvesting, RNA extraction (different extraction methods for different kinds of samples have been reported in the literature), mRNA enrichment, cDNA...
    33 KB (4,188 words) - 15:44, 5 March 2024
  • Thumbnail for Anne Wyllie
    require collection by healthcare workers. It also does not involve RNA extraction, allowing direct PCR testing of samples, and cutting the time required...
    16 KB (1,996 words) - 01:34, 4 April 2025
  • Thumbnail for Dry shipper
    Rebuffat, Sandra; Lajoix, Anne-Dominique (2021-10-25). "Optimization of RNA extraction methods from human metabolic tissue samples of the COMET biobank". Scientific...
    8 KB (743 words) - 17:13, 9 June 2025
  • Thumbnail for 4-Bromoanisole
    derivative CH3OC6H4ZnBr. 4-Bromoanisole is compound sometimes used in RNA extraction which serves to further eliminate DNA contamination. It interacts with...
    4 KB (222 words) - 20:05, 23 September 2024
  • Thumbnail for FLP-FRT recombination
    the progeny to reach adulthood before the progeny were sacrificed. RNA extraction was performed on neuronal, muscular, enteric, and tail tissue. Reverse-transcription...
    21 KB (2,655 words) - 10:31, 15 May 2024
  • Transcriptome (category RNA)
    techniques include DNA microarrays and RNA-Seq. Both techniques require RNA isolation through RNA extraction techniques, followed by its separation from...
    40 KB (4,861 words) - 01:44, 24 May 2025
  • useful in low-resource or field settings where a conventional DNA or RNA extraction prior to diagnostic testing may be impractical. LAMP has also been used...
    27 KB (3,191 words) - 14:30, 8 June 2025
  • Thumbnail for COVID-19 pandemic in Illinois
    2020). "Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step". PLOS Biology. 18 (10): e3000896. bioRxiv 10...
    170 KB (16,169 words) - 00:32, 21 June 2025
  • total RNA is isolated (both nuclear and cytoplasmic) by guanidinium thiocyanate-phenol-chloroform extraction (e.g. Trizol) which isolates most RNA (whereas...
    54 KB (5,405 words) - 16:14, 8 June 2025
  • Thumbnail for COVID-19 testing
    385–398. doi:10.1080/10408363.2021.1884640. PMC 7898297. PMID 33595397. "RNA Extraction". AssayGenie. Archived from the original on 6 May 2020. Retrieved 7...
    178 KB (20,620 words) - 22:42, 26 June 2025
  • and is commonly used as a component for lysing cells during RNA extraction or DNA extraction, inhibiting the activity of nucleases, enzymes that can degrade...
    29 KB (3,114 words) - 10:22, 28 June 2025
  • an automatic nuclear extraction machine. Government had ordered PCR machines for all 33 districts and automatic RNA extraction machines for remaining...
    150 KB (6,474 words) - 15:29, 3 April 2025