A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
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Reverse transcription polymerase chain reaction (redirect from Reverse Transcriptase quantitative PCR)
technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
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real time PCR with the use of allele specific probes. insertions and deletions are analyzed by dosage measurements using quantitative real time PCR.[citation...
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Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
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Digital polymerase chain reaction (redirect from Digital PCR)
PCR inhibitors or primer template mismatch. Real-time Digital PCR (rdPCR) combines the methodologies of digital PCR (dPCR) and quantitative PCR (qPCR)...
69 KB (7,351 words) - 04:46, 30 October 2024
Polymerase chain reaction (redirect from PCR oil)
qPCR is the appropriate contractions for quantitative PCR (real-time PCR). Reverse complement PCR (RC-PCR): Allows the addition of functional domains...
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A quantitative PCR instrument, also called real-time PCR machine, is an analytical instrument that amplifies and detects DNA. It combines the functions...
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"Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the National...
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Jean-Marc; La Scola, Bernard; Raoult, Didier (2012). "Comparison of real-time quantitative PCR and culture for the diagnosis of emerging Rickettsioses". PLOS...
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hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher David Gefland...
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the U.S. are performing screening for SCID in newborns using real-time quantitative PCR to measure the concentration of T-cell receptor excision circles...
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product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
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Bisulfite sequencing (redirect from Methylation specific PCR)
"Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection". Nucleic...
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Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real-Time Quantitative PCR and SYBR Green Chemistry". Journal of Clinical Microbiology. 39...
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Rocchi; Michele Zappella & Alessandra Renieri (August 2004). "Real-time quantitative PCR as a routine method for screening large rearrangements in Rett...
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sequence for the gene. Quantitative PCR (Q-PCR) is used to measure the quantity of a PCR product (preferably real-time, QRT-PCR). It is the method of choice...
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Potato virus Y (section Quantitative PCR)
advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
38 KB (5,658 words) - 06:18, 10 April 2025
McAlexander MA, Queen SE, Adams RJ (July 2013). "Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little...
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distance between the primer and the probe in some forms of real-time quantitative PCR (qPCR). The error rates of various polymerases (fidelity) have been...
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Multiplex polymerase chain reaction (redirect from Multiplex-PCR)
PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
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Resolution Melting (dHRM) is also used in conjunction with digital PCR (dPCR) to improve quantitative power by providing additional information on the melting behavior...
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Thermal cycler (redirect from PCR machine)
and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
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biotechnology company that designs and sells products for quantitative real-time polymerase chain reaction (qPCR). Primerdesign was founded in 2005 by Dr Jim Wicks...
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County in 2000, Viracor was among the first to commercially offer real-time quantitative PCR assays to diagnose patients with Adenovirus, BK virus and JC virus...
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expression studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated...
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previously limited to tracing individual transcripts by Northern blots or quantitative PCR. Higher throughput and speed allow researchers to frequently characterize...
48 KB (5,796 words) - 16:16, 18 April 2025
"PCR/Real-Time PCR Protocols". "Applied Biosystems - US" (PDF). O'Leary, J.J.; Sheils, O.; Martin, C.; Crowley, A. (2003). "Taqman Technology and Real-Time...
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"The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments". Clinical Chemistry. 55 (4): 611–622. doi:10.1373/clinchem...
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Shengting; Huang, Linkai (2019). "Reference gene selection for real-time quantitative PCR normalization in Hemarthria compressa and Hemarthria altissima...
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information Ahmad AI; Ghasemi JB (2007). "New FRET primers for quantitative real-time PCR". Anal Bioanal Chem. 387 (8): 2737–43. doi:10.1007/s00216-007-1123-4...
5 KB (470 words) - 11:26, 16 August 2024