• Thumbnail for Real-time polymerase chain reaction
    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
    43 KB (5,360 words) - 20:54, 17 February 2025
  • Thumbnail for Reverse transcription polymerase chain reaction
    technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
    45 KB (5,776 words) - 17:52, 21 March 2025
  • real time PCR with the use of allele specific probes. insertions and deletions are analyzed by dosage measurements using quantitative real time PCR.[citation...
    51 KB (5,957 words) - 01:14, 15 January 2025
  • Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
    20 KB (2,673 words) - 23:19, 8 April 2025
  • PCR inhibitors or primer template mismatch. Real-time Digital PCR (rdPCR) combines the methodologies of digital PCR (dPCR) and quantitative PCR (qPCR)...
    69 KB (7,351 words) - 04:46, 30 October 2024
  • Thumbnail for Polymerase chain reaction
    qPCR is the appropriate contractions for quantitative PCR (real-time PCR). Reverse complement PCR (RC-PCR): Allows the addition of functional domains...
    90 KB (11,271 words) - 23:49, 1 May 2025
  • Thumbnail for Quantitative PCR instrument
    A quantitative PCR instrument, also called real-time PCR machine, is an analytical instrument that amplifies and detects DNA. It combines the functions...
    11 KB (1,110 words) - 13:24, 3 April 2025
  • "Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the National...
    3 KB (334 words) - 13:39, 10 March 2025
  • Jean-Marc; La Scola, Bernard; Raoult, Didier (2012). "Comparison of real-time quantitative PCR and culture for the diagnosis of emerging Rickettsioses". PLOS...
    5 KB (454 words) - 13:53, 2 May 2025
  • hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher David Gefland...
    6 KB (684 words) - 21:36, 10 May 2025
  • Thumbnail for Severe combined immunodeficiency
    the U.S. are performing screening for SCID in newborns using real-time quantitative PCR to measure the concentration of T-cell receptor excision circles...
    34 KB (3,270 words) - 10:18, 10 May 2025
  • product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
    33 KB (4,155 words) - 13:49, 10 March 2025
  • Thumbnail for Bisulfite sequencing
    "Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection". Nucleic...
    35 KB (4,227 words) - 03:53, 2 December 2024
  • Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real-Time Quantitative PCR and SYBR Green Chemistry". Journal of Clinical Microbiology. 39...
    16 KB (1,899 words) - 07:46, 28 November 2024
  • Thumbnail for MECP2 duplication syndrome
    Rocchi; Michele Zappella & Alessandra Renieri (August 2004). "Real-time quantitative PCR as a routine method for screening large rearrangements in Rett...
    9 KB (849 words) - 23:49, 7 July 2024
  • sequence for the gene. Quantitative PCR (Q-PCR) is used to measure the quantity of a PCR product (preferably real-time, QRT-PCR). It is the method of choice...
    10 KB (1,401 words) - 12:18, 27 July 2024
  • advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
    38 KB (5,658 words) - 06:18, 10 April 2025
  • Thumbnail for Genetically modified food controversies
    McAlexander MA, Queen SE, Adams RJ (July 2013). "Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little...
    308 KB (32,800 words) - 22:51, 9 May 2025
  • Thumbnail for Thermostable DNA polymerase
    distance between the primer and the probe in some forms of real-time quantitative PCR (qPCR). The error rates of various polymerases (fidelity) have been...
    41 KB (5,164 words) - 18:26, 29 November 2024
  • PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
    7 KB (763 words) - 14:38, 12 February 2025
  • Resolution Melting (dHRM) is also used in conjunction with digital PCR (dPCR) to improve quantitative power by providing additional information on the melting behavior...
    12 KB (1,490 words) - 08:39, 26 February 2025
  • Thermal cycler (redirect from PCR machine)
    and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
    7 KB (556 words) - 21:34, 19 February 2025
  • Thumbnail for Primerdesign
    biotechnology company that designs and sells products for quantitative real-time polymerase chain reaction (qPCR). Primerdesign was founded in 2005 by Dr Jim Wicks...
    7 KB (590 words) - 06:59, 2 April 2025
  • County in 2000, Viracor was among the first to commercially offer real-time quantitative PCR assays to diagnose patients with Adenovirus, BK virus and JC virus...
    3 KB (247 words) - 01:56, 2 November 2023
  • expression studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated...
    17 KB (2,067 words) - 01:32, 3 December 2023
  • previously limited to tracing individual transcripts by Northern blots or quantitative PCR. Higher throughput and speed allow researchers to frequently characterize...
    48 KB (5,796 words) - 16:16, 18 April 2025
  • "PCR/Real-Time PCR Protocols". "Applied Biosystems - US" (PDF). O'Leary, J.J.; Sheils, O.; Martin, C.; Crowley, A. (2003). "Taqman Technology and Real-Time...
    32 KB (4,012 words) - 01:46, 9 December 2024
  • "The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments". Clinical Chemistry. 55 (4): 611–622. doi:10.1373/clinchem...
    9 KB (938 words) - 19:47, 31 March 2025
  • Thumbnail for Hemarthria altissima
    Shengting; Huang, Linkai (2019). "Reference gene selection for real-time quantitative PCR normalization in Hemarthria compressa and Hemarthria altissima...
    4 KB (202 words) - 09:57, 13 September 2024
  • Thumbnail for Texas Red
    information Ahmad AI; Ghasemi JB (2007). "New FRET primers for quantitative real-time PCR". Anal Bioanal Chem. 387 (8): 2737–43. doi:10.1007/s00216-007-1123-4...
    5 KB (470 words) - 11:26, 16 August 2024