A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
43 KB (5,360 words) - 23:36, 25 May 2025
Reverse transcription polymerase chain reaction (redirect from Real-time reverse transcription polymerase chain reaction)
technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
45 KB (5,776 words) - 17:52, 21 March 2025
beyond that of real-time PCR. Point mutations, loss of heterozygosity and aneuploidy can be detected in a single PCR step. Digital PCR can differentiate...
51 KB (5,957 words) - 22:26, 22 May 2025
Polymerase chain reaction (redirect from PCR oil)
real time. It is also sometimes abbreviated to RT-PCR (real-time PCR) but this abbreviation should be used only for reverse transcription PCR. qPCR is...
90 KB (11,293 words) - 13:08, 23 May 2025
Digital polymerase chain reaction (redirect from Digital PCR)
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction...
70 KB (7,425 words) - 17:07, 24 May 2025
Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments and...
20 KB (2,673 words) - 22:47, 23 May 2025
A quantitative PCR instrument, also called real-time PCR machine, is an analytical instrument that amplifies and detects DNA. It combines the functions...
11 KB (1,110 words) - 05:13, 23 May 2025
Relative fluorescence units (section DNA PCR analysis)
for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent...
5 KB (691 words) - 16:02, 17 September 2024
Gene doping (section Real time PCR)
limited capacity. Therefore, PCR can target these exon-exon junctions as a unique sequence that is not present in gDNA PCR has many applications in molecular...
32 KB (3,911 words) - 12:12, 25 May 2025
prior to PCR testing. Marth, Elmer H. (27 March 2007). Listeria, Listeriosis, and Food Safety. CRC Press. ISBN 9781420015188. "Real-Time PCR for Cutting-Edge...
2 KB (179 words) - 17:26, 23 April 2025
a pH buffer and come mixed in nuclease-free water. Master mixes for real-time PCR include a fluorescent compound (frequently SYBR green), and the choice...
3 KB (273 words) - 01:11, 30 April 2025
reverse transcription PCR (RT-PCR) tests have been developed for rabies but only been used as a confirmatory test. Real-time PCR-based tests which have...
14 KB (1,448 words) - 22:47, 15 March 2025
Czech Republic. Kubista has contributed to the field of quantitative real-time PCR (qPCR), with his work recognized as part of the early research in this...
23 KB (2,049 words) - 12:01, 28 May 2025
sensitivity of the real-time PCR based products without any additional analysis after the PCR amplification comparing to the traditional (PCR followed with...
26 KB (3,130 words) - 02:23, 16 April 2025
Alternatively, fluid collected from the rash may be analyzed for VZV DNA using real-time PCR. This test is rapid, easy to perform, and is highly sensitive and specific...
11 KB (1,020 words) - 12:57, 24 May 2025
Look up real-time, realtime, or real time in Wiktionary, the free dictionary. Real-time, realtime, or real time may refer to: Real-time computing, hardware...
4 KB (527 words) - 16:27, 18 April 2025
resistance of the serotype. Newer methods of "serotyping" include xMAP and real-time PCR, two methods based on DNA sequences instead of antibody reactions. These...
71 KB (8,225 words) - 23:37, 30 April 2025
quantitative data. Real-time PCR is useful in pathogen detection, gene expression and regulation, and allelic discrimination. This PCR technique utilizes...
135 KB (14,986 words) - 01:34, 19 May 2025
capable real-time PCR machine. HRM analysis is performed on double stranded DNA samples. Typically the user will use polymerase chain reaction (PCR) prior...
18 KB (2,581 words) - 21:26, 25 May 2025
during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods that...
33 KB (4,155 words) - 13:49, 10 March 2025
studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated expression...
17 KB (2,067 words) - 01:32, 3 December 2023
Pickett DO, Cohen MN, Moore EA, et al. (January 2010). "A quadruplex real-time PCR assay for rapid detection and differentiation of the Clostridium botulinum...
55 KB (6,003 words) - 18:03, 25 May 2025
diagnosis based on serology. However, newer laboratory techniques such as real-time PCR and microimmunofluorescence can be used to identify Rickettsia faster...
36 KB (3,996 words) - 19:28, 22 May 2025
COVID-19 testing (redirect from PCR testing procedure)
described as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used....
180 KB (20,995 words) - 00:42, 27 May 2025
not-for-profit organisation. Tests are by light microscopy and using real-time PCR for ruminant DNA. The presence of MBM and fish products in feed can...
12 KB (1,346 words) - 03:59, 16 January 2025
Minimum information standard (section MIQE, Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments". Clinical Chemistry. 55 (4): 611–622. doi:10.1373/clinchem...
17 KB (1,774 words) - 04:49, 11 January 2025
RT-PCR resources- primer databases, software, protocols Archived 2010-11-25 at the Wayback Machine 2. Beacon Designer - Software to design real time PCR...
6 KB (684 words) - 21:36, 10 May 2025
S2CID 30548375. Faize, M.; Faize, L.; Burgos, L. (2010). "Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor...
56 KB (6,402 words) - 10:53, 24 May 2025
Multiplex polymerase chain reaction (redirect from Multiplex-PCR)
John; Baker, Joffre; Sinicropi, Dominick (2009). "Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method". PLOS ONE. 4 (2): e4584...
7 KB (763 words) - 14:38, 12 February 2025
Glal hydrolysis and Ligation Adapter Dependent PCR assay (GLAD-PCR assay) is the novel method to determine R(5mC)GY sites produced in the course of de...
5 KB (636 words) - 01:48, 24 December 2024