• A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers...
    30 KB (3,823 words) - 22:13, 6 April 2025
  • Thumbnail for Fluorescence microscope
    A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption...
    25 KB (2,725 words) - 05:14, 1 April 2025
  • Thumbnail for Total internal reflection
    bright when viewed from the "external" side. In the total internal reflection fluorescence microscope (TIRFM), instead of relying on simple scattering,...
    107 KB (13,479 words) - 22:48, 7 January 2025
  • protein and DNA molecules for microscopy (as in the total internal reflection fluorescence microscope). The evanescent wave from an optical fiber can be...
    23 KB (3,164 words) - 17:09, 6 September 2024
  • limitations of traditional wide-field fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded...
    46 KB (5,308 words) - 02:06, 5 November 2024
  • TIRF may refer to: Total internal reflection fluorescence microscope Traffic Injury Research Foundation, in partnership with Toyota Canada Inc. Trans-inclusionary...
    249 bytes (54 words) - 01:00, 20 February 2020
  • Thumbnail for Microscopy
    excitation light entering the detector. See also: total internal reflection fluorescence microscope Neuroscience Confocal laser scanning microscopy uses...
    69 KB (8,316 words) - 20:02, 23 April 2025
  • Thumbnail for Fluorescence
    Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation...
    100 KB (11,053 words) - 15:51, 4 May 2025
  • confocal, total internal reflection fluorescence microscope, FRAP, etc. However, as an optical imaging technique, the development of fluorescence polarization...
    16 KB (2,043 words) - 21:36, 1 March 2023
  • microscopes allowing optical sectioning, such as total internal reflection fluorescence microscopes (TIRF) and light sheet fluorescence microscopes....
    27 KB (3,213 words) - 22:07, 18 May 2024
  • and phase-separated polymers and RNA/protein condensates. Total internal reflection fluorescence (TIRF) is a microscopy approach that is only sensitive to...
    63 KB (7,969 words) - 22:32, 15 March 2025
  • Super-resolution microscopy (category Fluorescence techniques)
    is mbPAINT ("mb" standing for motion blur). When a total internal reflection fluorescence microscope (TIRF) is used for imaging, the excitation depth is...
    89 KB (10,346 words) - 03:04, 14 April 2025
  • Thumbnail for Complete blood count
    blood smear review, in which the blood is stained and viewed under a microscope to verify that the analyzer results are consistent with the appearance...
    106 KB (12,446 words) - 06:39, 6 April 2025
  • Thumbnail for Optical sectioning
    these microscopes only interact with the surface of the sample. Total internal reflection microscopy is a fluorescent microscopy technique, which intentionally...
    11 KB (1,337 words) - 06:50, 3 March 2023
  • sufficient fluorescence signals to enable single-molecule detection. Wide-field multiphoton microscopy is typically combined with total internal reflection fluorescence...
    45 KB (6,137 words) - 15:28, 21 October 2024
  • Thumbnail for Diffraction-limited system
    Diffraction-limited system (category Microscopes)
    object. In fluorescence microscopy the excitation and emission are typically on different wavelengths. In total internal reflection fluorescence microscopy...
    20 KB (2,648 words) - 04:15, 5 May 2025
  • induce total internal reflection within the prism. Although the beam of light is not propagated through the surface of the refractive prism under total internal...
    26 KB (3,555 words) - 19:57, 28 December 2023
  • Thumbnail for Fluorescence imaging
    all utilize the same mechanism of fluorescence to observe a biological process. Total internal reflection fluorescence microscopy: A microscopy technique...
    15 KB (1,905 words) - 16:52, 25 September 2024
  • Thumbnail for Polarization (waves)
    polarization Spin angular momentum of light Optics Depolarizer (optics) Fluorescence anisotropy Glan–Taylor prism Kerr effect Nicol prism Pockels effect Polarization...
    74 KB (9,422 words) - 18:02, 5 March 2025
  • Thumbnail for Optical tweezers
    conventional fluorescence-activated cell sorting. An evanescent field is a residue optical field that "leaks" during total internal reflection. This "leaking"...
    72 KB (9,516 words) - 17:05, 29 March 2025
  • anaesthetic action Thomas A. Steitz Thomas Gold Thylakoid Total internal reflection fluorescence microscope Tradeoffs for locomotion in air and water Transepithelial...
    15 KB (1,545 words) - 18:32, 17 August 2024
  • Total external reflection Total internal reflection Total internal reflection fluorescence microscope Total pressure Total refraction Total variation diminishing...
    19 KB (2,020 words) - 14:34, 30 November 2024
  • physics - radiative heat transfer thin-film optics tilt (optics) total internal reflection transparency (optics) transverse mode triangular prism Tyndall...
    11 KB (898 words) - 23:45, 21 July 2024
  • X-ray fluorescence imaging. Polycapillary lenses are arrays of small hollow glass tubes that guide the X-rays with many total external reflections on the...
    21 KB (2,667 words) - 16:45, 1 November 2024
  • simulations. She uses a confocal super-resolution microscope and Total internal reflection fluorescence microscope to image dynamic events over long periods....
    7 KB (720 words) - 08:40, 15 March 2025
  • Thumbnail for Andor Technology
    sciences. Spinning disk confocal microscopes – for capturing high-speed live cell images Total internal reflection fluorescence (TIRF) – for studying cellular...
    19 KB (1,946 words) - 09:41, 3 March 2025
  • Thumbnail for Vertico spatially modulated illumination
    microscopy using transmission or reflection of natural light (as opposed to structured illumination or fluorescence) according to the Abbe resolution...
    18 KB (2,350 words) - 09:04, 8 March 2025
  • Thumbnail for Transparency and translucency
    may be lost via luminescence, fluorescence and phosphorescence. A molecule absorbs the photon, which results in reflection or scattering. A molecule cannot...
    34 KB (4,306 words) - 12:15, 5 March 2025
  • Thumbnail for X-ray spectroscopy
    absorption, fluorescence and atomic number). Atoms can be excited by a high-energy beam of charged particles such as electrons (in an electron microscope for...
    18 KB (2,659 words) - 10:57, 30 March 2025
  • Thumbnail for Terracotta Army
    experiments combined with energy-dispersive X-ray spectroscopy and micro-X-ray fluorescence analysis showed that the process of producing terracotta figures colored...
    84 KB (8,314 words) - 15:05, 1 April 2025