A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers...
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A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption...
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bright when viewed from the "external" side. In the total internal reflection fluorescence microscope (TIRFM), instead of relying on simple scattering,...
107 KB (13,479 words) - 22:48, 7 January 2025
protein and DNA molecules for microscopy (as in the total internal reflection fluorescence microscope). The evanescent wave from an optical fiber can be...
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Confocal microscopy (redirect from Confocal laser scanning microscope)
limitations of traditional wide-field fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded...
46 KB (5,308 words) - 02:06, 5 November 2024
TIRF may refer to: Total internal reflection fluorescence microscope Traffic Injury Research Foundation, in partnership with Toyota Canada Inc. Trans-inclusionary...
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Microscopy (section Fluorescence)
excitation light entering the detector. See also: total internal reflection fluorescence microscope Neuroscience Confocal laser scanning microscopy uses...
69 KB (8,316 words) - 20:02, 23 April 2025
Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation...
100 KB (11,053 words) - 15:51, 4 May 2025
confocal, total internal reflection fluorescence microscope, FRAP, etc. However, as an optical imaging technique, the development of fluorescence polarization...
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microscopes allowing optical sectioning, such as total internal reflection fluorescence microscopes (TIRF) and light sheet fluorescence microscopes....
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and phase-separated polymers and RNA/protein condensates. Total internal reflection fluorescence (TIRF) is a microscopy approach that is only sensitive to...
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Super-resolution microscopy (category Fluorescence techniques)
is mbPAINT ("mb" standing for motion blur). When a total internal reflection fluorescence microscope (TIRF) is used for imaging, the excitation depth is...
89 KB (10,346 words) - 03:04, 14 April 2025
Complete blood count (redirect from Total RBC count)
blood smear review, in which the blood is stained and viewed under a microscope to verify that the analyzer results are consistent with the appearance...
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these microscopes only interact with the surface of the sample. Total internal reflection microscopy is a fluorescent microscopy technique, which intentionally...
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sufficient fluorescence signals to enable single-molecule detection. Wide-field multiphoton microscopy is typically combined with total internal reflection fluorescence...
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Diffraction-limited system (category Microscopes)
object. In fluorescence microscopy the excitation and emission are typically on different wavelengths. In total internal reflection fluorescence microscopy...
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induce total internal reflection within the prism. Although the beam of light is not propagated through the surface of the refractive prism under total internal...
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all utilize the same mechanism of fluorescence to observe a biological process. Total internal reflection fluorescence microscopy: A microscopy technique...
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polarization Spin angular momentum of light Optics Depolarizer (optics) Fluorescence anisotropy Glan–Taylor prism Kerr effect Nicol prism Pockels effect Polarization...
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conventional fluorescence-activated cell sorting. An evanescent field is a residue optical field that "leaks" during total internal reflection. This "leaking"...
72 KB (9,516 words) - 17:05, 29 March 2025
anaesthetic action Thomas A. Steitz Thomas Gold Thylakoid Total internal reflection fluorescence microscope Tradeoffs for locomotion in air and water Transepithelial...
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Total external reflection Total internal reflection Total internal reflection fluorescence microscope Total pressure Total refraction Total variation diminishing...
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physics - radiative heat transfer thin-film optics tilt (optics) total internal reflection transparency (optics) transverse mode triangular prism Tyndall...
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X-ray optics (section Reflection)
X-ray fluorescence imaging. Polycapillary lenses are arrays of small hollow glass tubes that guide the X-rays with many total external reflections on the...
21 KB (2,667 words) - 16:45, 1 November 2024
simulations. She uses a confocal super-resolution microscope and Total internal reflection fluorescence microscope to image dynamic events over long periods....
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sciences. Spinning disk confocal microscopes – for capturing high-speed live cell images Total internal reflection fluorescence (TIRF) – for studying cellular...
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Vertico spatially modulated illumination (category Fluorescence)
microscopy using transmission or reflection of natural light (as opposed to structured illumination or fluorescence) according to the Abbe resolution...
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may be lost via luminescence, fluorescence and phosphorescence. A molecule absorbs the photon, which results in reflection or scattering. A molecule cannot...
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absorption, fluorescence and atomic number). Atoms can be excited by a high-energy beam of charged particles such as electrons (in an electron microscope for...
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experiments combined with energy-dispersive X-ray spectroscopy and micro-X-ray fluorescence analysis showed that the process of producing terracotta figures colored...
84 KB (8,314 words) - 15:05, 1 April 2025