A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of...

complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both...

There are different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and...

one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a...

amplifying a library of such in bacterial plasmids. In 1982–1983, the idea of selecting random or semi-random clones from such a cDNA library for sequencing...

hybrids between RNA and DNA, and opens the door to modern tools such as cDNA libraries. While most complementarity is seen between two separate strings of...

in the film. This method allows further RNA transcript profiling and cDNA library generation of the retrieved cells. RNA sequencing of the selected regions...

immunoprecipitation. The resulting cDNA library was subsequently screened via sequence analysis and compared to a whole genome library for confirmation of specific...

similar celerity. The general steps to prepare a complementary DNA (cDNA) library for sequencing are described below, but often vary between platforms...

templates, to generate cDNA products for next-generation sequencing (NGS). Overall, OTTR offers a streamlined approach for cDNA library production of full-length...

W.; Koehler, M. D.; Efstratiadis, A.; Maniatis, T. (1979). "Use of a cDNA library for studies on evolution and developmental expression of the chorion...

Cells for High-Efficiency Plasmid Transformation of Escherichia coli". CDNA Library Protocols. Methods in Molecular Biology. Vol. 69. pp. 129–138. doi:10...

convert single-stranded RNA into double-stranded cDNA. In retroviruses and retrotransposons, this cDNA can then integrate into the host genome, from which...

The single-cell RNA-seq technique converts a population of RNAs to a library of cDNA fragments. Single cells are labeled by adding beads with barcoded oligonucleotides;...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

to screen against the W.bancrofti Larval stage 3 cDNA library. This led to the identification of a cDNA sequence of W.bancrofti SXP-1 that encoded for a...

kinase refers to the first cloning of a SGK family member from a cDNA library screen for genes upregulated by the glucocorticoid dexamethasone in a...

to a company associated with Open Biosystems. complementary DNA (cDNA) cDNA library expressed sequence tags (EST) vector (molecular biology) cloning vector...

transcription. The early stages of transcriptome annotations began with cDNA libraries published in the 1980s. Subsequently, the advent of high-throughput...

(September 1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10...

high-salt wash, SDS-PAGE, membrane transfer, proteinase digestion, cDNA library preparation and sequencing in order to identify the direct RNA binding...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

clones is the cDNA library. This generates a snap-shot of tissue mRNA that is stable and can be sequenced at a later stage. The cDNA library must be sequenced...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

the CDNA 3 architecture, which is optimized for high-performance computing (HPC) and generative artificial intelligence (AI) workloads. The CDNA 3 architecture...

and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149...

about 8kb with 13 exon sequences. It was successfully cloned from a cDNA library in 1995 revealing that, after processing, it is 477 nucleotides long...

obtain the cDNA for a specific gene, it is first necessary to construct a cDNA library. The DNA of interest needs to be fragmented to provide a relevant DNA...