• Thumbnail for Plasmid
    A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They...
    69 KB (7,776 words) - 04:07, 2 June 2025
  • Thumbnail for Ti plasmid
    A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including A. tumefaciens, A. rhizogenes, A. rubi and A. vitis...
    46 KB (5,743 words) - 23:15, 23 September 2024
  • the plasmid copy number is the number of copies of a given plasmid in a cell. To ensure survival and thus the continued propagation of the plasmid, they...
    9 KB (1,235 words) - 09:54, 8 October 2021
  • Thumbnail for Plasmid preparation
    A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and...
    18 KB (2,038 words) - 07:32, 7 January 2025
  • cryptic plasmid is a plasmid that doesn't appear to provide any clear advantage to its host, yet still persists in bacterial populations. These plasmids appear...
    3 KB (289 words) - 06:54, 3 March 2025
  • The F-plasmid (first named F by one of its discoverers Esther Lederberg;also called the sex factor in E. coli,the F sex factor, the fertility factor,...
    10 KB (1,223 words) - 00:38, 19 February 2025
  • (T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a vir helper plasmid. The two plasmids are used together (thus binary)...
    41 KB (4,361 words) - 11:35, 22 May 2025
  • Thumbnail for Plasmid-mediated resistance
    Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. Plasmids possess mechanisms that ensure their...
    25 KB (2,791 words) - 03:34, 26 May 2025
  • A plasmid partition system is a mechanism that ensures the stable inheritance of plasmids during bacterial cell division. Each plasmid has its independent...
    19 KB (2,276 words) - 04:07, 25 November 2024
  • The RK2 Plasmid is a broad-host-range plasmid belonging to the incP incompatibility group It is notable for its ability to replicate in a wide variety...
    9 KB (1,276 words) - 22:37, 17 May 2025
  • Thumbnail for Genetic transformation
    tumor-inducing agent was found to be a DNA plasmid called the Ti plasmid. By removing the genes in the plasmid that caused the tumor and adding in novel...
    55 KB (6,818 words) - 22:30, 1 April 2025
  • conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain...
    3 KB (355 words) - 10:11, 25 May 2025
  • Thumbnail for Molecular cloning
    inserted into a plasmid and that these foreign sequences would be carried into bacteria and digested as part of the plasmid. That is, these plasmids could serve...
    32 KB (4,017 words) - 04:13, 12 April 2025
  • Thumbnail for Multiple cloning site
    engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. The purpose of an MCS in a plasmid is to...
    34 KB (3,833 words) - 19:47, 22 May 2025
  • Thumbnail for R1 plasmid
    The R1 plasmid is a plasmid that was first isolated from Salmonella paratyphi bacteria in 1963. It is a short plasmid, composed of 97,566 nucleotides and...
    11 KB (1,312 words) - 21:45, 25 May 2025
  • In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
    18 KB (2,164 words) - 18:27, 4 June 2024
  • vector, which has both bacteriophage and plasmid properties. These vectors carry, in addition to the origin of plasmid replication, an origin of replication...
    5 KB (679 words) - 18:05, 18 December 2024
  • Thumbnail for Bacterial conjugation
    or mobilizable genetic element that is most often a plasmid or transposon. Most conjugative plasmids have systems ensuring that the recipient cell does...
    22 KB (2,419 words) - 03:04, 2 January 2025
  • Thumbnail for Expression vector
    expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce...
    34 KB (4,182 words) - 17:22, 7 December 2024
  • Thumbnail for Subcloning
    the destination plasmid. The plasmid is often transformed into a bacterium like E. coli. Ideally when the bacterium divides the plasmid should also be...
    5 KB (631 words) - 10:53, 31 October 2024
  • The root inducing (Ri) -plasmid of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a plasmid capable of undergoing horizontal gene transfer...
    4 KB (532 words) - 05:27, 13 June 2023
  • Thumbnail for DNA vaccine
    immune response. DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune...
    77 KB (8,378 words) - 06:14, 23 May 2025
  • of isolating plasmid deoxyribonucleic acid (DNA) in bacteria. It is a standard method used in molecular biology to isolate the plasmid without obtaining...
    9 KB (1,169 words) - 16:47, 24 May 2025
  • Thumbnail for Horizontal gene transfer
    transmission of virulence. It often involves temperate bacteriophages and plasmids. Genes responsible for antibiotic resistance in one species of bacteria...
    128 KB (13,959 words) - 12:33, 22 May 2025
  • Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used...
    11 KB (1,399 words) - 21:08, 24 May 2025
  • The diagram shows the effects of nicks on intersecting DNA in a twisted plasmid. Nicking can be used to dissipate the energy held up by intersecting states...
    16 KB (2,060 words) - 03:54, 3 February 2025
  • Thumbnail for Transfer DNA
    tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes (actually an Ri plasmid). The T-DNA is...
    14 KB (1,865 words) - 13:07, 26 May 2025
  • the plasmid. Usually, the restriction enzymes that cut at the plasmid and the oligonucleotide are the same, permitting sticky ends of the plasmid and...
    25 KB (2,980 words) - 11:45, 6 February 2025
  • Thumbnail for Drug resistance
    peptide bonds Plasmid encode enzymes that chemically alter the drug (e.g., by acetylation or phosphorylation), thereby inactivating it. Plasmid encode beta-lactamase...
    28 KB (2,627 words) - 20:39, 29 May 2025
  • Thumbnail for Hfr cell
    bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome...
    6 KB (622 words) - 20:50, 23 February 2025