• Thumbnail for Plasmid
    A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They...
    69 KB (7,776 words) - 22:27, 24 May 2025
  • Thumbnail for Plasmid preparation
    A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and...
    18 KB (2,038 words) - 07:32, 7 January 2025
  • Thumbnail for Ti plasmid
    A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including A. tumefaciens, A. rhizogenes, A. rubi and A. vitis...
    46 KB (5,743 words) - 23:15, 23 September 2024
  • The F-plasmid (first named F by one of its discoverers Esther Lederberg;also called the sex factor in E. coli,the F sex factor, the fertility factor,...
    10 KB (1,223 words) - 00:38, 19 February 2025
  • cryptic plasmid is a plasmid that doesn't appear to provide any clear advantage to its host, yet still persists in bacterial populations. These plasmids appear...
    3 KB (289 words) - 06:54, 3 March 2025
  • the plasmid copy number is the number of copies of a given plasmid in a cell. To ensure survival and thus the continued propagation of the plasmid, they...
    9 KB (1,235 words) - 09:54, 8 October 2021
  • Thumbnail for Plasmid-mediated resistance
    Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. Plasmids possess mechanisms that ensure their...
    25 KB (2,791 words) - 03:34, 26 May 2025
  • (T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a vir helper plasmid. The two plasmids are used together (thus binary)...
    41 KB (4,361 words) - 11:35, 22 May 2025
  • conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain...
    3 KB (355 words) - 10:11, 25 May 2025
  • Thumbnail for Horizontal gene transfer
    transmission of virulence. It often involves temperate bacteriophages and plasmids. Genes responsible for antibiotic resistance in one species of bacteria...
    128 KB (13,959 words) - 12:33, 22 May 2025
  • The root inducing (Ri) -plasmid of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a plasmid capable of undergoing horizontal gene transfer...
    4 KB (532 words) - 05:27, 13 June 2023
  • Thumbnail for Multiple cloning site
    engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. The purpose of an MCS in a plasmid is to...
    34 KB (3,833 words) - 19:47, 22 May 2025
  • Thumbnail for DNA vaccine
    immune response. DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune...
    77 KB (8,378 words) - 06:14, 23 May 2025
  • Thumbnail for Genetic transformation
    tumor-inducing agent was found to be a DNA plasmid called the Ti plasmid. By removing the genes in the plasmid that caused the tumor and adding in novel...
    55 KB (6,818 words) - 22:30, 1 April 2025
  • vector, which has both bacteriophage and plasmid properties. These vectors carry, in addition to the origin of plasmid replication, an origin of replication...
    5 KB (679 words) - 18:05, 18 December 2024
  • Thumbnail for Molecular cloning
    inserted into a plasmid and that these foreign sequences would be carried into bacteria and digested as part of the plasmid. That is, these plasmids could serve...
    32 KB (4,017 words) - 04:13, 12 April 2025
  • Thumbnail for Bacterial conjugation
    or mobilizable genetic element that is most often a plasmid or transposon. Most conjugative plasmids have systems ensuring that the recipient cell does...
    22 KB (2,419 words) - 03:04, 2 January 2025
  • The RK2 Plasmid is a broad-host-range plasmid belonging to the incP incompatibility group It is notable for its ability to replicate in a wide variety...
    9 KB (1,276 words) - 22:37, 17 May 2025
  • In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
    18 KB (2,164 words) - 18:27, 4 June 2024
  • Addgene is a non-profit plasmid repository. Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated...
    10 KB (861 words) - 17:03, 28 May 2025
  • Thumbnail for R1 plasmid
    The R1 plasmid is a plasmid that was first isolated from Salmonella paratyphi bacteria in 1963. It is a short plasmid, composed of 97,566 nucleotides and...
    11 KB (1,312 words) - 21:45, 25 May 2025
  • Thumbnail for Subcloning
    the destination plasmid. The plasmid is often transformed into a bacterium like E. coli. Ideally when the bacterium divides the plasmid should also be...
    5 KB (631 words) - 10:53, 31 October 2024
  • Thumbnail for CRISPR gene editing
    recover transduced cells. There are two plasmid systems in CRISPR/Cas9 libraries. First, is all in one plasmid, where sgRNA and Cas9 are produced simultaneously...
    177 KB (20,524 words) - 22:37, 29 May 2025
  • efficiency. They are defined by three mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening. The cells are...
    2 KB (260 words) - 07:58, 13 May 2024
  • Thumbnail for Cloning vector
    Cloning vector (category Plasmids)
    genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages...
    20 KB (2,517 words) - 17:11, 5 November 2024
  • Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used...
    11 KB (1,399 words) - 21:08, 24 May 2025
  • Hybrid plasmid is a plasmid that contains an inserted piece of foreign DNA. "Everything Bio : Hybrid plasmid". Everythingbio.com. Retrieved 2013-11-15...
    370 bytes (26 words) - 16:10, 22 July 2022
  • of isolating plasmid deoxyribonucleic acid (DNA) in bacteria. It is a standard method used in molecular biology to isolate the plasmid without obtaining...
    9 KB (1,169 words) - 16:47, 24 May 2025
  • the plasmid. Usually, the restriction enzymes that cut at the plasmid and the oligonucleotide are the same, permitting sticky ends of the plasmid and...
    25 KB (2,980 words) - 11:45, 6 February 2025
  • Thumbnail for Natural competence
    provided by the researcher, often as a genetically engineered fragment or plasmid. During uptake, DNA is transported across the cell membrane(s), and the...
    17 KB (1,906 words) - 03:36, 5 August 2024