A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They...
69 KB (7,776 words) - 04:07, 2 June 2025
A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including A. tumefaciens, A. rhizogenes, A. rubi and A. vitis...
46 KB (5,743 words) - 23:15, 23 September 2024
the plasmid copy number is the number of copies of a given plasmid in a cell. To ensure survival and thus the continued propagation of the plasmid, they...
9 KB (1,235 words) - 09:54, 8 October 2021
A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and...
18 KB (2,038 words) - 07:32, 7 January 2025
cryptic plasmid is a plasmid that doesn't appear to provide any clear advantage to its host, yet still persists in bacterial populations. These plasmids appear...
3 KB (289 words) - 06:54, 3 March 2025
The F-plasmid (first named F by one of its discoverers Esther Lederberg;also called the sex factor in E. coli,the F sex factor, the fertility factor,...
10 KB (1,223 words) - 00:38, 19 February 2025
Transfer DNA binary system (redirect from Binary plasmid)
(T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a vir helper plasmid. The two plasmids are used together (thus binary)...
41 KB (4,361 words) - 11:35, 22 May 2025
Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. Plasmids possess mechanisms that ensure their...
25 KB (2,791 words) - 03:34, 26 May 2025
A plasmid partition system is a mechanism that ensures the stable inheritance of plasmids during bacterial cell division. Each plasmid has its independent...
19 KB (2,276 words) - 04:07, 25 November 2024
The RK2 Plasmid is a broad-host-range plasmid belonging to the incP incompatibility group It is notable for its ability to replicate in a wide variety...
9 KB (1,276 words) - 22:37, 17 May 2025
tumor-inducing agent was found to be a DNA plasmid called the Ti plasmid. By removing the genes in the plasmid that caused the tumor and adding in novel...
55 KB (6,818 words) - 22:30, 1 April 2025
conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain...
3 KB (355 words) - 10:11, 25 May 2025
inserted into a plasmid and that these foreign sequences would be carried into bacteria and digested as part of the plasmid. That is, these plasmids could serve...
32 KB (4,017 words) - 04:13, 12 April 2025
engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. The purpose of an MCS in a plasmid is to...
34 KB (3,833 words) - 19:47, 22 May 2025
The R1 plasmid is a plasmid that was first isolated from Salmonella paratyphi bacteria in 1963. It is a short plasmid, composed of 97,566 nucleotides and...
11 KB (1,312 words) - 21:45, 25 May 2025
Vector (molecular biology) (section Plasmids)
In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
18 KB (2,164 words) - 18:27, 4 June 2024
vector, which has both bacteriophage and plasmid properties. These vectors carry, in addition to the origin of plasmid replication, an origin of replication...
5 KB (679 words) - 18:05, 18 December 2024
or mobilizable genetic element that is most often a plasmid or transposon. Most conjugative plasmids have systems ensuring that the recipient cell does...
22 KB (2,419 words) - 03:04, 2 January 2025
expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce...
34 KB (4,182 words) - 17:22, 7 December 2024
Subcloning (section Amplification of product plasmid)
the destination plasmid. The plasmid is often transformed into a bacterium like E. coli. Ideally when the bacterium divides the plasmid should also be...
5 KB (631 words) - 10:53, 31 October 2024
The root inducing (Ri) -plasmid of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a plasmid capable of undergoing horizontal gene transfer...
4 KB (532 words) - 05:27, 13 June 2023
DNA vaccine (section Plasmid vectors)
immune response. DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune...
77 KB (8,378 words) - 06:14, 23 May 2025
of isolating plasmid deoxyribonucleic acid (DNA) in bacteria. It is a standard method used in molecular biology to isolate the plasmid without obtaining...
9 KB (1,169 words) - 16:47, 24 May 2025
transmission of virulence. It often involves temperate bacteriophages and plasmids. Genes responsible for antibiotic resistance in one species of bacteria...
128 KB (13,959 words) - 12:33, 22 May 2025
Plant transformation vector (section Plasmid selection)
Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used...
11 KB (1,399 words) - 21:08, 24 May 2025
The diagram shows the effects of nicks on intersecting DNA in a twisted plasmid. Nicking can be used to dissipate the energy held up by intersecting states...
16 KB (2,060 words) - 03:54, 3 February 2025
tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes (actually an Ri plasmid). The T-DNA is...
14 KB (1,865 words) - 13:07, 26 May 2025
the plasmid. Usually, the restriction enzymes that cut at the plasmid and the oligonucleotide are the same, permitting sticky ends of the plasmid and...
25 KB (2,980 words) - 11:45, 6 February 2025
Drug resistance (redirect from Drug-resistance plasmid)
peptide bonds Plasmid encode enzymes that chemically alter the drug (e.g., by acetylation or phosphorylation), thereby inactivating it. Plasmid encode beta-lactamase...
28 KB (2,627 words) - 20:39, 29 May 2025
bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome...
6 KB (622 words) - 20:50, 23 February 2025