Reverse complement polymerase chain reaction (RC-PCR) is a modification of the polymerase chain reaction (PCR). It is primarily used to generate amplicon...
14 KB (1,544 words) - 15:15, 18 July 2025
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called...
45 KB (5,779 words) - 15:50, 15 July 2025
The polymerase chain reaction (PCR) is a laboratory method widely used to amplify copies of specific DNA sequences rapidly, to enable detailed study....
91 KB (11,378 words) - 13:28, 18 July 2025
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods...
70 KB (7,425 words) - 06:06, 2 June 2025
Once an antibody–reaction has taken place in a test, other methods are needed to confirm this. Older methods included complement fixation tests, hemagglutination...
59 KB (7,227 words) - 12:25, 16 July 2025
DNA replication (redirect from Leading chain)
at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction (LCR), and transcription-mediated amplification...
61 KB (7,458 words) - 16:22, 17 July 2025
Complementarity (molecular biology) (redirect from Reverse complement)
basis of commonly performed laboratory techniques such as the polymerase chain reaction, PCR. Two strands of complementary sequence are referred to as...
23 KB (2,586 words) - 10:40, 20 January 2025
Bisulfite sequencing (redirect from Methylation-specific polymerase chain reaction)
methylation-specific PCR (MSP) (Figure 4), and strategies employing polymerase chain reaction (PCR) performed under non-methylation-specific conditions (Figure...
35 KB (4,229 words) - 12:59, 12 June 2025
Molecular biology (section Polymerase chain reaction)
DNA sequence coding for a protein of interest is cloned using polymerase chain reaction (PCR), and/or restriction enzymes, into a plasmid (expression...
50 KB (5,675 words) - 08:18, 18 July 2025
Complementary DNA (section Reverse transcription)
amplification of DNA sequences via polymerase chain reaction (PCR) now commonplace, one will typically conduct reverse transcription as an initial step...
19 KB (2,275 words) - 04:33, 26 May 2025
(KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific oligo extension and fluorescence...
7 KB (988 words) - 17:44, 16 July 2025
real-time polymerase chain reaction, synonymous with quantitative PCR. 2. An abbreviation of reverse transcription polymerase chain reaction. Contents...
301 KB (26,725 words) - 15:05, 3 July 2025
are introduced during sample preparation and sequencing such as polymerase chain reaction, sequencing, and image analysis errors. While the NGS platforms'...
18 KB (2,276 words) - 12:19, 14 May 2024
immunofluorescence, enzyme immunoassays or fluoroimmunoassays. Polymerase chain reaction (PCR). Analysis of specific IgG antibodies showing a subsequent...
30 KB (3,086 words) - 23:36, 25 May 2025
using antibody testing, viral cultures, or real-time reverse transcription polymerase chain reaction may be needed. There is no specific treatment for mumps...
63 KB (7,859 words) - 08:44, 18 July 2025
tectonics – point mutation – pollenizer – pollination – pollinator – polymerase chain reaction – polypeptide – polyploidy – polysaccharide – population – population...
19 KB (1,371 words) - 11:27, 22 March 2025
DNA, as the signal is too weak to measure. This is overcome by polymerase chain reaction (PCR) amplification. Once a nucleic acid sequence has been obtained...
22 KB (2,409 words) - 18:07, 16 July 2025
amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain reaction or ligase...
340 KB (31,622 words) - 15:06, 3 July 2025
DNA strand during a polymerase chain reaction. The term is also often used to describe the reformation (renaturation) of reverse-complementary strands...
29 KB (3,397 words) - 16:13, 14 July 2025
11 beta-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and localization of the type 2 isoform to renal collecting...
11 KB (1,357 words) - 15:27, 17 July 2025
pneumonia (formerly Pneumocystis carinii or PCP) – POL – polymerase – polymerase chain reaction (PCR) – polyneuritis – polypeptide – polyvalent vaccine...
23 KB (1,720 words) - 22:45, 3 January 2023
factor - platelet-derived growth factor receptor - polymer - polymerase chain reaction - polymerization - polymyxin - polymyxin B - polyomavirus transforming...
29 KB (2,469 words) - 23:55, 12 June 2025
Molecular genetics (section Reverse genetics)
screens to relate genotypic sequences to phenotypes. Polymerase chain reaction (PCR) using Taq polymerase, invented by Mullis in 1985, enabled scientists to...
32 KB (3,845 words) - 08:18, 18 July 2025
and unmodified protein; NAD+ is not restored by the reverse reaction. Poly(ADP-ribose)polymerases (PARPs) are found mostly in eukaryotes and catalyze...
29 KB (3,545 words) - 06:50, 27 February 2025
Analytical profile index (category Biochemistry detection reactions)
of bacterial species and strains. These newer methods can be used to complement or even replace the use of API testing in clinical settings. The analytical...
7 KB (806 words) - 17:13, 12 November 2024
patients with oropouche meningoencephalitis confirmed by reverse transcription-polymerase chain reaction, two of the patients had underlying conditions that...
22 KB (2,442 words) - 20:00, 1 July 2025
it is synthesised as the complement of the template strand. Transcription is performed by an enzyme called an RNA polymerase, which reads the template...
100 KB (11,213 words) - 16:40, 17 July 2025
S2CID 19341821. "Human Immunodeficiency Virus, Type 1 (HIV-1) Reverse Transcription (RT) Polymerase Chain Reaction (PCR) Assay". Fda.gov. 2007-01-31. Archived from...
33 KB (3,085 words) - 03:36, 11 July 2025
virus in nasopharyngeal swabs and aspirates. NAAT assays such as polymerase chain reaction (PCR) detect virus-specific genetic material, rather than viral...
98 KB (9,646 words) - 00:35, 13 July 2025
2012). "Exon skipping quantification by quantitative reverse-transcription polymerase chain reaction in Duchenne muscular dystrophy patients treated with...
22 KB (1,490 words) - 19:03, 16 July 2025