• Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between...
    29 KB (3,433 words) - 02:47, 31 May 2025
  • However, liquid chromatography techniques exist that do utilize affinity chromatography properties. Immobilized metal affinity chromatography (IMAC) is useful...
    58 KB (7,243 words) - 05:40, 23 May 2025
  • Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...
    11 KB (1,327 words) - 11:09, 21 January 2025
  • can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...
    30 KB (3,951 words) - 16:33, 14 April 2025
  • Thumbnail for Affinity electrophoresis
    affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by...
    19 KB (2,124 words) - 20:41, 22 May 2025
  • Thumbnail for Size-exclusion chromatography
    Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by...
    31 KB (3,901 words) - 01:50, 24 May 2025
  • affinity Étienne François Geoffroy — Geoffroy's 1718 Affinity Table Valency Affinity chromatography Affinity electrophoresis Chisholm 1911, Affinity,...
    10 KB (1,341 words) - 19:24, 14 August 2024
  • Thumbnail for High-performance liquid chromatography
    High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used...
    89 KB (10,969 words) - 20:19, 22 May 2025
  • Thumbnail for His-tag
    using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC)...
    22 KB (2,655 words) - 04:36, 16 October 2024
  • Thumbnail for Column chromatography
    available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The...
    18 KB (2,378 words) - 02:45, 29 March 2025
  • Thumbnail for Meir Wilchek
    biorecognition or affinity phenomenon, and its various application, e.g. for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin...
    15 KB (1,665 words) - 14:40, 21 May 2025
  • Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that...
    19 KB (2,639 words) - 18:13, 15 December 2023
  • Thumbnail for Organomercury chemistry
    a soft electrophile. This mode of action makes them useful for affinity chromatography to separate thiol-containing compounds from complex mixtures. For...
    17 KB (1,744 words) - 22:39, 2 June 2025
  • (November 2020). "Affinity chromatography: A review of trends and developments over the past 50 years". Journal of Chromatography. B, Analytical Technologies...
    63 KB (6,159 words) - 14:33, 30 November 2024
  • Thumbnail for Fast protein liquid chromatography
    As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a...
    16 KB (2,274 words) - 06:50, 25 May 2025
  • Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly...
    8 KB (1,054 words) - 16:14, 13 March 2025
  • Thumbnail for Liquid chromatography–mass spectrometry
    adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these...
    46 KB (5,943 words) - 11:41, 25 May 2025
  • Thumbnail for Paper chromatography
    Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be...
    8 KB (1,099 words) - 08:45, 3 April 2025
  • size, and binding affinity. Here are the main types of chromatography used in protein purification: Ion exchange chromatography separates proteins based...
    19 KB (2,284 words) - 02:33, 2 June 2025
  • biotin) for the affinity isolation of labeled proteins/peptides. The samples are combined and then separated through chromatography, then sent through...
    4 KB (404 words) - 23:51, 9 December 2023
  • Thumbnail for Lectin
    and carbohydrate structure by means of affinity chromatography, blotting, affinity electrophoresis, and affinity immunoelectrophoreis with lectins, as...
    30 KB (3,037 words) - 01:01, 21 May 2025
  • suggestion of common descent or type Affinity chromatography, method of separating a biomolecule from a mixture Affinity electrophoresis, general name for...
    4 KB (434 words) - 12:55, 28 May 2025
  • Thumbnail for Agarose
    scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous...
    20 KB (2,443 words) - 17:35, 19 September 2024
  • Thumbnail for Ion chromatography
    chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity...
    54 KB (6,995 words) - 23:21, 23 May 2025
  • high-performance liquid chromatography, immunoassay, enzymatic assay, capillary electrophoresis, or boronate affinity chromatography. Point of care (e.g....
    44 KB (4,974 words) - 23:26, 12 May 2025
  • Thumbnail for Heparin
    50 μL reaction mixture. Heparin has been used as a chromatography resin, acting as both an affinity ligand and an ion exchanger. Its polyanionic structure...
    78 KB (7,850 words) - 21:03, 29 May 2025
  • affinity purification using phosphospecific antibodies, immobilized metal affinity chromatography (IMAC), strong cation exchange (SCX) chromatography...
    13 KB (1,541 words) - 21:05, 15 March 2025
  • Size-exclusion chromatography (SEC) Affinity chromatography Centrifugal partition chromatography Gas chromatography and Inverse gas chromatography Crystallization...
    8 KB (936 words) - 04:17, 28 May 2025
  • Thumbnail for Affitin
    utilized in biochemical purification techniques, specifically in affinity chromatography. The ability of Affitins to selectively bind antigens is used to...
    6 KB (758 words) - 15:17, 20 September 2024
  • Thumbnail for Imidazole
    affinity chromatography (IMAC). Imidazole is used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column...
    25 KB (2,551 words) - 18:15, 6 June 2025