Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between...
29 KB (3,431 words) - 01:16, 28 May 2025
However, liquid chromatography techniques exist that do utilize affinity chromatography properties. Immobilized metal affinity chromatography (IMAC) is useful...
58 KB (7,243 words) - 05:40, 23 May 2025
Protein purification (section Affinity chromatography)
can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...
30 KB (3,951 words) - 16:33, 14 April 2025
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used...
89 KB (10,969 words) - 20:19, 22 May 2025
available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The...
18 KB (2,378 words) - 02:45, 29 March 2025
using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC)...
22 KB (2,655 words) - 04:36, 16 October 2024
affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by...
19 KB (2,124 words) - 20:41, 22 May 2025
Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...
11 KB (1,327 words) - 11:09, 21 January 2025
Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by...
31 KB (3,901 words) - 01:50, 24 May 2025
Chemoproteomics (section Affinity chromatography)
(November 2020). "Affinity chromatography: A review of trends and developments over the past 50 years". Journal of Chromatography. B, Analytical Technologies...
63 KB (6,159 words) - 14:33, 30 November 2024
affinity Étienne François Geoffroy — Geoffroy's 1718 Affinity Table Valency Affinity chromatography Affinity electrophoresis Chisholm 1911, Affinity,...
10 KB (1,341 words) - 19:24, 14 August 2024
a soft electrophile. This mode of action makes them useful for affinity chromatography to separate thiol-containing compounds from complex mixtures. For...
16 KB (1,744 words) - 21:30, 27 May 2025
Protein methods (section Affinity Chromatography)
size, and binding affinity. Here are the main types of chromatography used in protein purification: Ion exchange chromatography separates proteins based...
19 KB (2,284 words) - 04:51, 9 November 2024
As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a...
16 KB (2,274 words) - 06:50, 25 May 2025
adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these...
46 KB (5,943 words) - 11:41, 25 May 2025
Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be...
8 KB (1,099 words) - 08:45, 3 April 2025
Meir Wilchek (section Affinity chromatography)
biorecognition or affinity phenomenon, and its various application, e.g. for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin...
15 KB (1,665 words) - 14:40, 21 May 2025
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly...
8 KB (1,054 words) - 16:14, 13 March 2025
suggestion of common descent or type Affinity chromatography, method of separating a biomolecule from a mixture Affinity electrophoresis, general name for...
4 KB (440 words) - 19:13, 8 November 2023
scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous...
20 KB (2,443 words) - 17:35, 19 September 2024
high-performance liquid chromatography, immunoassay, enzymatic assay, capillary electrophoresis, or boronate affinity chromatography. Point of care (e.g....
44 KB (4,974 words) - 23:26, 12 May 2025
Size-exclusion chromatography (SEC) Affinity chromatography Centrifugal partition chromatography Gas chromatography and Inverse gas chromatography Crystallization...
8 KB (936 words) - 04:17, 28 May 2025
and carbohydrate structure by means of affinity chromatography, blotting, affinity electrophoresis, and affinity immunoelectrophoreis with lectins, as...
30 KB (3,037 words) - 01:01, 21 May 2025
50 μL reaction mixture. Heparin has been used as a chromatography resin, acting as both an affinity ligand and an ion exchanger. Its polyanionic structure...
78 KB (7,850 words) - 12:28, 23 May 2025
monoclonal antibodies are often purified using Protein A/G or antigen-affinity chromatography. In research, purified antibodies are used in many applications...
126 KB (13,957 words) - 16:00, 26 May 2025
method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino...
8 KB (1,016 words) - 22:58, 24 February 2025
for example affinity chromatography (or even tandem affinity purification), size exclusion chromatography, ion exchange chromatography. Proteins can...
42 KB (5,319 words) - 14:29, 2 November 2024
hydrophobic interaction chromatography, size exclusion chromatography, ion exchange chromatography, and affinity chromatography separations as well as...
20 KB (2,770 words) - 19:30, 2 December 2023
It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells...
21 KB (2,275 words) - 12:42, 19 May 2025
or a hexa-his peptide (6xHis-tag), which can be isolated using affinity chromatography with nickel or cobalt resins. Di- or multimeric chimeric proteins...
19 KB (2,305 words) - 05:22, 15 May 2024