Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between...
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However, liquid chromatography techniques exist that do utilize affinity chromatography properties. Immobilized metal affinity chromatography (IMAC) is useful...
58 KB (7,243 words) - 05:40, 23 May 2025
Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...
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Protein purification (section Affinity chromatography)
can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...
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affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by...
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Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by...
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affinity Étienne François Geoffroy — Geoffroy's 1718 Affinity Table Valency Affinity chromatography Affinity electrophoresis Chisholm 1911, Affinity,...
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High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used...
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using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC)...
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available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The...
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Meir Wilchek (section Affinity chromatography)
biorecognition or affinity phenomenon, and its various application, e.g. for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin...
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Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that...
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a soft electrophile. This mode of action makes them useful for affinity chromatography to separate thiol-containing compounds from complex mixtures. For...
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Chemoproteomics (section Affinity chromatography)
(November 2020). "Affinity chromatography: A review of trends and developments over the past 50 years". Journal of Chromatography. B, Analytical Technologies...
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As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a...
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Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly...
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adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these...
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Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be...
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Protein methods (section Affinity Chromatography)
size, and binding affinity. Here are the main types of chromatography used in protein purification: Ion exchange chromatography separates proteins based...
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biotin) for the affinity isolation of labeled proteins/peptides. The samples are combined and then separated through chromatography, then sent through...
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and carbohydrate structure by means of affinity chromatography, blotting, affinity electrophoresis, and affinity immunoelectrophoreis with lectins, as...
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suggestion of common descent or type Affinity chromatography, method of separating a biomolecule from a mixture Affinity electrophoresis, general name for...
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scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous...
20 KB (2,443 words) - 17:35, 19 September 2024
chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity...
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high-performance liquid chromatography, immunoassay, enzymatic assay, capillary electrophoresis, or boronate affinity chromatography. Point of care (e.g....
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50 μL reaction mixture. Heparin has been used as a chromatography resin, acting as both an affinity ligand and an ion exchanger. Its polyanionic structure...
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affinity purification using phosphospecific antibodies, immobilized metal affinity chromatography (IMAC), strong cation exchange (SCX) chromatography...
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Size-exclusion chromatography (SEC) Affinity chromatography Centrifugal partition chromatography Gas chromatography and Inverse gas chromatography Crystallization...
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utilized in biochemical purification techniques, specifically in affinity chromatography. The ability of Affitins to selectively bind antigens is used to...
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affinity chromatography (IMAC). Imidazole is used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column...
25 KB (2,551 words) - 18:15, 6 June 2025