Guanidinium thiocyanate (GTC) or guanidinium isothiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent...

6 M guanidinium chloride, almost all proteins lose their entire secondary structure and become randomly coiled peptide chains. Guanidinium thiocyanate is...

Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology...

Chomczyński and Nicoletta Sacchi in 1987. TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies, and Tri-Reagent is the...

hydrogen sulfide, and carbon disulfide, leaving a residue of guanidinium thiocyanate. [NH4]+[SCN]− is weakly acidic due to the ammonium ion; it reacts...

Comprehensive Cancer Center. She is the co-discoverer of the acid guanidinium thiocyanate-phenol-chloroform extraction method to extract RNA from biological...

to be the first to discover the high denaturing action of guanidinium halides and thiocyanates in following the liberation of sulfhydryl groups in ovalbumin...

under alkaline conditions. This was later improved using guanidinium thiocyanate or guanidinium hydrochloride as the chaotropic agent. For ease of handling...

the experiment: acid guanidinium thiocyanate-phenol-chloroform extraction: it is based on the use of a guanidinium-thiocyanate solution combined with...

CRISPR. Piotr Chomczynski and Nicoletta Sacchi publish their acid guanidinium thiocyanate-phenol-chloroform extraction protocol. Larry Wall releases the...

Sacchi N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Analytical Biochemistry. 162 (1):...

addition of 0.5–1% sodium dodecyl sulfate (SDS) or Guanidinium chloride (3 M), Guanidinium thiocyanate (1 M) and urea (4 M) [disputed (for: no source cited...

between tubes, it is at a greater risk of contamination. Acid guanidinium thiocyanate-phenol-chloroform extraction Ethanol precipitation Spin column-based...

Bromochloropropane, a chemical used in RNA and DNA isolation; see acid guanidinium thiocyanate-phenol-chloroform extraction Bromocresol purple, a pH indicator...

this test (1 hour treatment). Only chlorine, phenolic compounds, guanidinium thiocyanate, and sodium hydroxide reduce prion levels by more than 4 logs;...

Sacchi N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Analytical Biochemistry. 162 (1):...

differential cytotoxicity against cancer cells. Biology portal Guanidinium thiocyanate - a chemical RNase inhibitor. PDB: 2BNH​; Kobe B, Deisenhofer J...

example total RNA is isolated (both nuclear and cytoplasmic) by guanidinium thiocyanate-phenol-chloroform extraction (e.g. Trizol) which isolates most...

biology to isolate RNA from samples, the most common of these is guanidinium thiocyanate-phenol-chloroform extraction. Usually, the phenol-chloroform solution...

"Nanometer-Scale Ion Aggregates in Aqueous Electrolyte Solutions: Guanidinium Sulfate and Guanidinium Thiocyanate". The Journal of Physical Chemistry B. 109 (50): 24185–24196...

first-hand opportunity to study the virus, which was inactivated with guanidinium thiocyanate before transport. This sample and others found in AFIP archives...

Cruickshank, J. M. (8 April 2003). "The hydration structure of guanidinium and thiocyanate ions: Implications for protein stability in aqueous solution"...

to the original Boom method, the chaotropic guanidinium salt employed is preferably guanidinium thiocyanate (GuSCN). According to the chaotropic effect...

first-hand opportunity to study the virus, which was inactivated with guanidinium thiocyanate before transport. This sample and others found in U.S. Armed Forces...

(alpha-GPC), a natural choline compound found in the brain Acid guanidinium thiocyanate-phenol-chloroform extraction, a liquid–liquid extraction technique...

Cations Onium ions Carbenium ions Others Guanidinium C(NH2)+3 Tropylium C7H+7 Mercury(I) Hg2+2 Ammonium NH+4 Triphenylcarbenium (C6H5)3C+ Dihydrogen H+2...

Kenneth W. (2013-12-12). Th Thorium: Compounds with Carbon: Carbonates, Thiocyanates, Alkoxides, Carboxylates. Springer Science & Business Media. pp. 98–102...