The T7 expression system is utilized in the field of microbiology to clone recombinant DNA using strains of E. coli. It is the most popular system for...
6 KB (798 words) - 19:55, 18 July 2025
strain that uses a T7 expression system driven by the tightly regulated arabinose-inducible PBAD promoter. It fixes the leaky expression issue seen in BL21...
8 KB (864 words) - 02:56, 19 May 2025
T7 RNA polymerase is used in the synthesis of mRNA and sgRNA. T7 expression system "T7 RNA Polymerase (20 U/µL)". www.thermofisher.com. Retrieved 2023-01-19...
9 KB (1,030 words) - 18:30, 12 June 2024
Studier FW (1991). "Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor". Journal...
34 KB (4,183 words) - 03:54, 15 July 2025
Recombinant DNA (section DNA expression)
Host cell protein T7 expression system Rosano, Germán L.; Ceccarelli, Eduardo A. (17 April 2014). "Recombinant protein expression in Escherichia coli:...
34 KB (3,860 words) - 16:51, 22 July 2025
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these...
17 KB (1,851 words) - 02:14, 24 July 2025
Protein production (redirect from Expression system)
prophage found in BL21(DE3) provides T7 RNA polymerase (driven by the LacUV5 promoter), allowing for vectors with the T7 promoter to be used instead. Non-pathogenic...
25 KB (2,654 words) - 08:14, 28 October 2024
Kathleen S. (2000). "Generation of an AraC-araBAD Promoter-Regulated T7 Expression System". Analytical Biochemistry. 277 (1): 67–73. doi:10.1006/abio.1999...
8 KB (792 words) - 05:56, 3 June 2024
parasympathetic nervous system (PSNS) is one of the three divisions of the autonomic nervous system, the others being the sympathetic nervous system and the enteric...
31 KB (3,871 words) - 01:13, 16 December 2024
sympathetic nervous system (SNS or SANS, sympathetic autonomic nervous system, to differentiate it from the somatic nervous system) is one of the three...
32 KB (3,737 words) - 21:24, 22 July 2025
total sleep and their circadian expression of PER2, an element of the SCN pacemaker, was not disrupted. Through the T7 cycle, the mice were exposed to...
30 KB (3,389 words) - 13:03, 14 July 2025
Operon (redirect from Inducible system)
bacteriophages. For example, T7 phages have two operons. The first operon codes for various products, including a special T7 RNA polymerase which can bind...
23 KB (2,712 words) - 18:01, 22 July 2025
nervous system. All afferent touch/vibration information ascends the spinal cord via the dorsal column-medial lemniscus pathway via gracilis (T7 and below)...
40 KB (4,575 words) - 07:20, 19 July 2025
soluble expression, the N-terminus of a T7 polymerase was fused to APOBEC1, with the remaining portion of the polymerase separately expressed. The T7 polymerase...
14 KB (1,436 words) - 03:04, 16 December 2024
Phage display (section T7 phages)
bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also been used. Phage display was first described by George...
38 KB (4,638 words) - 14:37, 22 July 2025
T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands...
22 KB (2,552 words) - 13:56, 17 July 2025
tool in the expression of toxic recombinant proteins. Expressing recombinant proteins in BL21(DE3) strains is typically accomplished by the T7-RNA-polymerase...
38 KB (4,306 words) - 07:34, 12 July 2025
streptactin (Strep-tag II: WSHPQFEK) T7-tag, an epitope tag derived from the T7 major capsid protein of the T7 gene (MASMTGGQQMG). Used in different...
24 KB (2,695 words) - 08:11, 23 July 2025
Cloning vector (section Elements for expression)
proteins are only produced when required. Some commonly used promoters are the T7 and lac promoters. The presence of a promoter is necessary when screening...
20 KB (2,517 words) - 20:55, 1 July 2025
instruction set architectures (ISA): A-1 : 19 developed by MIPS Computer Systems, now MIPS Technologies, based in the United States. There are multiple...
70 KB (8,083 words) - 19:26, 27 July 2025
bacteriophage T7, E. coli thioredoxin forms a complex with T7 DNA polymerase, which results in enhanced T7 DNA replication, a crucial step for successful T7 infection...
47 KB (5,108 words) - 00:55, 26 May 2025
library has a T7 RNA polymerase transcription site and a ribosomal binding site at the 5’ end. The T7 promoter region allows large-scale in vitro T7 transcription...
16 KB (2,214 words) - 17:06, 8 June 2024
Triple-stranded DNA (section Gene expression)
triplex DNA formation impairs the expression of intron 1 of the FXN gene. This results in the degeneration of the nervous system and spinal cord, impairing the...
48 KB (6,098 words) - 09:02, 24 June 2025
the interaction of the three membrane proteins with each other and EssC. T7 dependent effector proteins EsaD is DNA endonuclease toxin secreted by S....
128 KB (13,968 words) - 07:41, 21 July 2025
Bacteriophage (section Systems biology)
Bacteriophages are known to interact with the immune system both indirectly via bacterial expression of phage-encoded proteins and directly by influencing...
85 KB (9,494 words) - 06:16, 27 July 2025
experiments in eukaryotic cells and organisms with inducible protein expression systems using tetracycline-controlled transcriptional activation. The mechanism...
97 KB (9,351 words) - 10:36, 17 July 2025
bacteriophage T7 RNA polymerase in 1985. Richardson used the T7 RNA polymerase/promoter system to control the expression of a phage T7 gene 5 protein...
18 KB (1,954 words) - 23:54, 23 May 2025
RNA interference (category Gene expression)
interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi". Molecular and Biochemical Parasitology. 133...
140 KB (15,415 words) - 15:34, 18 July 2025
Axial tilt (section Solar System bodies)
05″ t6 + 7.12″ t7 + 27.87″ t8 + 5.79″ t9 + 2.45″ t10 where here t is multiples of 10,000 Julian years from J2000.0. These expressions are for the so-called...
32 KB (3,070 words) - 09:15, 4 July 2025
resistance; some BACs also have lacZ at the cloning site for blue/white selection. T7 & Sp6 phage promoters for transcription of inserted genes. BACs are now being...
8 KB (957 words) - 08:17, 21 July 2025