Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a...

first approach for developing a human gene therapy procedure, standard microinjection techniques were modified to permit the injection of DNA plasmids into...

biolistics and electroporation is lower than agrobacterial transformation. Microinjection is where DNA is injected through the cell's nuclear envelope directly...

Molly, the only demonstrated way to make a transgenic animal was by microinjection of DNA into the pronuclei of fertilized oocytes (eggs). However, only...

chemical, and biological. Physical methods include electroporation, microinjection, gene gun, impalefection, hydrostatic pressure, continuous infusion...

activation that mimics sperm entry, such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide...

cells can be reintegrated into the host. Despite the advantages of microinjection, the process is time consuming as each cell must be individually injected...

available, such as calcium phosphate transfection, electroporation, microinjection and liposome transfection. The plasmid may be integrated into the genome...

extrachromosomal DNA. DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope directly...

transmission. The axons of lamprey are particularly large and allow for microinjection of substances for experimental manipulation. They are also capable of...

embryos in 1980. Most of the very first transmutations were performed by microinjection of DNA directly into cells. Scientists were able to develop other methods...

confers advantages over other physical delivery methods, including microinjection and gene gun techniques. However, it is limited by reduced cell viability...

approach has also been used to generate knockin rats by TALEN mRNA microinjection in one-cell embryos. TALEN has also been utilized experimentally to...

expression. Glutamic acid has been implicated in epileptic seizures. Microinjection of glutamic acid into neurons produces spontaneous depolarisations around...

subregions of each area, either opioid-stimulating or orexin-stimulating microinjections appear to enhance the number of liking reactions elicited by sweetness...

autopsy, so he wears a double-layered rubber suit and scientists use microinjection techniques to deliver embalming fluids. According to anthropologist...

Agrobacteria Biolistics Electroporation Genetic transduction Lipofection Microinjection Somatic cell nuclear transfer Transfection Types Recombinant DNA Transgenesis...

physically interact with microscopic samples, such as in the procedures of microinjection and patch clamping. Most micropipettes are made of borosilicate, aluminosilicate...

easily evaluated on the basis of sperm motility in most cases. The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional...

Perforated Patch Recording", Methods in Neurosciences, Electrophysiology and Microinjection, vol. 4, Academic Press, pp. 364–373, doi:10.1016/B978-0-12-185257-3...

PMID 2464685. O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM (September 1989). "Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing...

microinjection of oxytocin receptor antagonist had lower social recognition scores than rats that received a vehicle injection, and microinjections of...

on the Y chromosome. Transgenic mice generated through exogenous DNA microinjection of an embryo's pronucleus are also considered to be hemizygous, because...

mutations. This results in embryos with targeted gene knockout. Standard microinjection techniques allow this technology to make knockout rats in 4–6 months...

forces to deliver DNA to a cell. It is claimed to be more effective than microinjection because the lance used is ten times smaller than a micropipette and...

the nuclear splice-modifying activity of Morpholinos observed after microinjection into the cytosol of cells. Different methods are used for delivery into...

stress. Microinjections of a GABA-a antagonist into the nucleus raphe pallidus, induces an increased heart rate. Conversely, microinjections of muscimol...

electroporation. DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope directly...

the invention of biolistics, Agrobacterium-mediated recombination and microinjection. The first genetically modified animal was a mouse created in 1974 by...

infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection". Insect Biochemistry and Molecular Biology. 35 (8): 903–910. doi:10...