• Thumbnail for DNA polymerase
    A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors...
    59 KB (7,090 words) - 09:35, 13 June 2024
  • Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement. J Biol Chem...
    9 KB (1,064 words) - 22:45, 12 January 2024
  • Thumbnail for DNA clamp
    of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand....
    23 KB (2,044 words) - 07:04, 10 March 2024
  • Thumbnail for DNA ligase
    in vitro activity of T4 DNA ligase; one successful approach, for example, tested T4 DNA ligase fused to several alternative DNA binding proteins and found...
    23 KB (2,787 words) - 06:43, 12 May 2024
  • Thumbnail for DNA replication
    synthesized partner strand. DNA polymerases are a family of enzymes that carry out all forms of DNA replication. DNA polymerases in general cannot initiate...
    60 KB (7,287 words) - 09:34, 2 June 2024
  • in an ATP dependent manner while double-stranded DNA is being unwound by elongating RNA-polymerase or by helicase in front of the progressing replication...
    15 KB (1,871 words) - 05:54, 24 August 2023
  • Thumbnail for DNA virus
    A DNA virus is a virus that has a genome made of deoxyribonucleic acid (DNA) that is replicated by a DNA polymerase. They can be divided between those...
    25 KB (2,893 words) - 04:22, 4 July 2023
  • Thumbnail for Prokaryotic DNA replication
    priming is complete, DNA polymerase III holoenzyme is loaded into the DNA and replication begins. The catalytic mechanism of DNA polymerase III involves the...
    17 KB (2,150 words) - 13:43, 23 June 2023
  • Thumbnail for Hi-C (genomic analysis technique)
    using phenol-chloroform DNA extraction. To remove any fragments with biotin-labeled ends that have not been ligated, T4 DNA Polymerase with 3’ to 5’ exonuclease...
    98 KB (10,972 words) - 12:56, 11 June 2024
  • Thumbnail for DNA sequencing
    determine the sequencing of a DNA sample by detecting the incorporation of a nucleotide by a DNA polymerase. An engineered polymerase is used to synthesize a...
    129 KB (14,413 words) - 10:39, 30 May 2024
  • dnaQ is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli. The ε subunit is one of three core proteins in the DNA polymerase complex...
    9 KB (1,154 words) - 12:56, 18 August 2023
  • Thumbnail for Deoxyadenosine triphosphate
    Romaniuk PJ, Eckstein F (July 1982). "A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate"...
    8 KB (653 words) - 16:18, 4 September 2023
  • Thumbnail for Aptamer
    the term SELEX for their process of selecting RNA ligands against T4 DNA polymerase and the lab of Jack Szostak, selecting RNA ligands against various...
    60 KB (6,560 words) - 00:03, 20 April 2024
  • Thumbnail for Okazaki fragments
    Okazaki fragments (category DNA replication)
    helicase, creating what is known as the DNA replication fork. Following this fork, DNA primase and DNA polymerase begin to act in order to create a new...
    33 KB (4,435 words) - 14:24, 7 June 2024
  • Thumbnail for Chloroplast DNA
    RNA polymerase atp-dependent protease ribosomal proteins tRNAs nicotiana tabacum edit · image Chloroplast DNA Interactive gene map of chloroplast DNA from...
    54 KB (5,883 words) - 08:32, 30 May 2024
  • Thumbnail for Thermostable DNA polymerase
    Thermostable DNA polymerases are DNA polymerases that originate from thermophiles, usually bacterial or archaeal species, and are therefore thermostable...
    39 KB (4,921 words) - 08:12, 13 June 2024
  • Thumbnail for Ligation (molecular biology)
    DNA ligase dates back to 1967 and is an important event in the field of molecular biology. Ligation in the laboratory is normally performed using T4 DNA...
    38 KB (4,862 words) - 03:50, 15 May 2024
  • Thumbnail for Rolling circle replication
    a primer for DNA synthesis by DNA polymerase III. Using the unnicked strand as a template, replication proceeds around the circular DNA molecule, displacing...
    24 KB (2,920 words) - 06:07, 28 September 2023
  • Thumbnail for Systematic evolution of ligands by exponential enrichment
    of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase". Science. 249 (4968): 505–10. Bibcode:1990Sci...249..505T. doi:10...
    41 KB (4,741 words) - 05:07, 22 April 2024
  • Thumbnail for Lambda phage
    Lambda phage (redirect from Lambda DNA)
    cannot occur from circular phage DNA, only from concatomeric DNA. Q is similar to N in its effect: Q binds to RNA polymerase in Qut sites and the resulting...
    54 KB (5,929 words) - 07:41, 3 June 2024
  • Thumbnail for Stephen J. Benkovic
    of the T4 DNA polymerase during replication, Proc Natl. Acad. Sci. USA 101, 8289-8294. Benkovic, S.J., Spiering, M.M. (2017) “Understanding DNA Replication...
    31 KB (3,256 words) - 06:15, 27 May 2024
  • of the DNA duplex during DNA replication and transcription. If left unchanged, this torsion would eventually stop the DNA or RNA polymerases involved...
    57 KB (6,173 words) - 00:11, 11 December 2023
  • Thumbnail for Lytic cycle
    modify the host's transcriptional process by RNA polymerase. Amongst other modifications, virus T4 changes the sigma factor of the host by producing...
    9 KB (1,355 words) - 00:15, 14 June 2024
  • Thumbnail for DNA adenine methylase
    DNA polymerase makes an error resulting in a mismatched base-pair or a small insertion or deletion during DNA synthesis, the cell will repair the DNA...
    16 KB (1,844 words) - 17:37, 28 November 2023
  • Thumbnail for DNA shuffling
    I is utilized. The ends of the fragments are made blunt by adding T4 DNA polymerase. Blunting the fragments is important for combining the fragments as...
    25 KB (2,913 words) - 04:36, 21 September 2023
  • Thumbnail for Replisome
    Replisome (category DNA replication)
    replicative polymerase with respect to its mismatch sensing activity. The replication of bacteriophage T4 DNA upon infection of E. coli is a well-studied DNA replication...
    32 KB (3,700 words) - 00:38, 1 January 2024
  • Thumbnail for Origin of replication
    viruses utilize host cell DNA polymerases, which attach to a viral origin of replication if the T antigen is present. Although DNA replication is essential...
    113 KB (12,561 words) - 17:02, 14 May 2024
  • Variants of PCR (category Polymerase chain reaction)
    cycle, and therefore is not commonly used in PCR. The bacteriophage T4 DNA polymerase (family A) was also initially used in PCR. It has a higher fidelity...
    32 KB (4,102 words) - 05:25, 7 February 2024
  • Thumbnail for Pyrimidine dimer
    translesion polymerases, and replication and or transcription machinery can continue past the lesion. One specific translesion DNA polymerase, DNA polymerase η...
    16 KB (1,941 words) - 19:50, 15 June 2024
  • block DNA replication.[citation needed] Exposure of bacteriophage (phage) T4 to ultraviolet irradiation induces thymine dimers in the phage DNA. The phage...
    25 KB (2,688 words) - 21:18, 6 April 2024