Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a...

first approach for developing a human gene therapy procedure, standard microinjection techniques were modified to permit the injection of DNA plasmids into...

biolistics and electroporation is lower than agrobacterial transformation. Microinjection is where DNA is injected through the cell's nuclear envelope directly...

cells can be reintegrated into the host. Despite the advantages of microinjection, the process is time consuming as each cell must be individually injected...

Microinjection of antibodies to GRASP65 prevents normal Golgi stack formation....

Molly, the only demonstrated way to make a transgenic animal was by microinjection of DNA into the pronuclei of fertilized oocytes (eggs). However, only...

approach has also been used to generate knockin rats by TALEN mRNA microinjection in one-cell embryos. TALEN has also been utilized experimentally to...

transmission. The axons of lamprey are particularly large and allow for microinjection of substances for experimental manipulation. They are also capable of...

the invention of biolistics, Agrobacterium-mediated recombination and microinjection. The first genetically modified animal was a mouse created in 1974 by...

available, such as calcium phosphate transfection, electroporation, microinjection and liposome transfection. The plasmid may be integrated into the genome...

embryos in 1980. Most of the very first transmutations were performed by microinjection of DNA directly into cells. Scientists were able to develop other methods...

forces to deliver DNA to a cell. It is claimed to be more effective than microinjection because the lance used is ten times smaller than a micropipette and...

chemical, and biological. Physical methods include electroporation, microinjection, gene gun, impalefection, hydrostatic pressure, continuous infusion...

subregions of each area, either opioid-stimulating or orexin-stimulating microinjections appear to enhance the number of liking reactions elicited by sweetness...

only method and had sort of aids available at the time – microinjection. In microinjection, DNA is injected into the pronuclei of fertilized oocytes...

extrachromosomal DNA. DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope directly...

mutations. This results in embryos with targeted gene knockout. Standard microinjection techniques allow this technology to make knockout rats in 4–6 months...

transgenic procedure for generation of stably transgenic lines by embryo microinjection. Hydra polyps are small and transparent which makes it possible to trace...

confers advantages over other physical delivery methods, including microinjection and gene gun techniques. However, it is limited by reduced cell viability...

stress. Microinjections of a GABA-a antagonist into the nucleus raphe pallidus, induces an increased heart rate. Conversely, microinjections of muscimol...

expression. Glutamic acid has been implicated in epileptic seizures. Microinjection of glutamic acid into neurons produces spontaneous depolarisations around...

activation that mimics sperm entry, such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide...

that frog oocytes arrested in G2 could be induced to enter M phase by microinjection of cytoplasm from oocytes that had been hormonally stimulated with progesterone...

autopsy, so he wears a double-layered rubber suit and scientists use microinjection techniques to deliver embalming fluids. According to anthropologist...

sequence. The construct can be delivered to stem cells either through microinjection or electroporation. This method then relies on the cell's own repair...

Agrobacteria Biolistics Electroporation Genetic transduction Lipofection Microinjection Somatic cell nuclear transfer Transfection Types Recombinant DNA Transgenesis...

easily evaluated on the basis of sperm motility in most cases. The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional...

and Ruddle F.H. (1980). "Genetic transformation of mouse embryos by microinjection of purified DNA". Proc. Natl. Acad. Sci. USA. 77 (12): 7380–7384. Bibcode:1980PNAS...

electroporation. DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope directly...

the nuclear splice-modifying activity of Morpholinos observed after microinjection into the cytosol of cells. Different methods are used for delivery into...