• A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition...
    55 KB (5,856 words) - 08:25, 13 May 2025
  • a restriction digest, DNA molecules are cleaved at specific regions of 4-12 nucleotides in length (restriction sites) by use of restriction enzymes which...
    6 KB (785 words) - 05:51, 9 June 2025
  • Restriction enzyme mediated integration (abbreviated as REMI) is a technique for integrating DNA (linearised plasmid) into the genome sites that have been...
    4 KB (426 words) - 07:50, 22 February 2024
  • Thumbnail for Golden Gate Cloning
    piece using Type IIS restriction enzymes and T4 DNA ligase. This assembly is performed in vitro. Most commonly used Type IIS enzymes include BsaI, BsmBI...
    18 KB (2,496 words) - 18:18, 31 October 2024
  • regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences...
    25 KB (2,688 words) - 05:28, 20 June 2025
  • recognized by restriction enzymes, which cleave the DNA at or near the site. These are generally palindromic sequences (because restriction enzymes usually...
    5 KB (654 words) - 05:29, 9 June 2025
  • Thumbnail for Restriction site associated DNA markers
    sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome. Once RAD tags have been isolated...
    13 KB (1,424 words) - 17:28, 9 June 2025
  • polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences...
    9 KB (1,163 words) - 11:15, 11 February 2025
  • list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of...
    80 KB (3,046 words) - 23:44, 27 August 2024
  • such as that borne by bacteriophages. Bacteria have restriction enzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific...
    20 KB (2,668 words) - 19:17, 21 May 2025
  • Thumbnail for Thermus aquaticus
    Thermus aquaticus arose at this time from the convention of giving restriction enzymes short names, such as Sal and Hin, derived from the genus and species...
    15 KB (1,802 words) - 22:27, 5 February 2025
  • Thumbnail for Multiple cloning site
    is designing complementary oligonucleotide sequences that contain restriction enzyme sites along with additional bases on the end that are complementary...
    34 KB (3,833 words) - 19:47, 22 May 2025
  • Thumbnail for Palindromic sequence
    palindrome. Many restriction endonucleases (restriction enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes...
    7 KB (825 words) - 20:55, 9 December 2024
  • Thumbnail for EcoRI
    EcoRI (category Restriction enzymes)
    (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices...
    8 KB (891 words) - 05:50, 8 February 2025
  • declarations Restriction enzyme, a type of enzyme that cleaves genetic material Restriction, a term in medieval supposition theory Restriction (mathematics)...
    854 bytes (129 words) - 03:28, 9 September 2024
  • A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria...
    8 KB (1,087 words) - 08:23, 12 September 2023
  • Thumbnail for Nuclease
    of the two types of enzymes responsible for phage growth restriction in Escherichia coli (E. coli) bacteria. One of these enzymes added a methyl group...
    22 KB (2,538 words) - 09:39, 13 February 2025
  • A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes, which cleave...
    5 KB (623 words) - 05:29, 9 June 2025
  • Thumbnail for Reduced representation bisulfite sequencing
    genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a...
    13 KB (1,786 words) - 15:27, 8 June 2025
  • Thumbnail for Southern blot
    from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by electrophoresis...
    17 KB (2,313 words) - 21:59, 23 May 2025
  • by restriction enzymes that break the molecule's phosphodiester backbone at specific locations, which themselves belong to a larger class of enzymes called...
    9 KB (1,247 words) - 10:27, 17 November 2024
  • mixture of PCR amplified variants of a single gene using one or more restriction enzymes and detecting the size of each of the individual resulting terminal...
    15 KB (1,966 words) - 01:41, 11 November 2024
  • Thumbnail for Restriction fragment
    molecular biology, a restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases)....
    3 KB (364 words) - 05:36, 9 June 2025
  • Thumbnail for DNA shuffling
    recombination or the similarity of the DNA sequences, restriction enzymes which rely on common restriction sites, and nonhomologous random recombination which...
    25 KB (2,913 words) - 04:36, 21 September 2023
  • studied restriction enzymes whose names start with O to R inclusive. It contains approximately 130 enzymes. The following information is given: Enzyme: Accepted...
    41 KB (654 words) - 23:27, 19 January 2023
  • Isoschizomer (category Restriction enzymes)
    Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers...
    2 KB (245 words) - 04:50, 16 September 2023
  • studied restriction enzymes whose names start with C to D inclusive. It contains approximately 80 enzymes. The following information is given: Enzyme: Accepted...
    27 KB (676 words) - 23:26, 19 January 2023
  • DpnI (category Restriction enzymes)
    interactions. DpnII restriction endonuclease family, also isolated from S. pneumonae List of restriction enzyme cutting sites Restriction modification system...
    5 KB (512 words) - 18:48, 27 May 2025
  • Thumbnail for Hamilton O. Smith
    Zurich. In 1970, Smith and Kent W. Wilcox discovered the first type II restriction enzyme, which is now known as HindII. Smith went on to discover DNA methylases...
    7 KB (638 words) - 16:23, 13 May 2025
  • studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes. The following information is given: Enzyme: Accepted...
    37 KB (616 words) - 23:27, 19 January 2023