Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA. DNA extraction Phenol–chloroform extraction Minicolumn purification...
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Nucleic acids are large biomolecules that are crucial in all cells and viruses. They are composed of nucleotides, which are the monomer components: a...
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In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture,...
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A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession...
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column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will...
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DNA (redirect from Deoxiribose nucleic acid)
and ribonucleic acid (RNA) are nucleic acids. Alongside proteins, lipids and complex carbohydrates (polysaccharides), nucleic acids are one of the four...
167 KB (17,915 words) - 22:22, 29 May 2025
The nucleic acid notation currently in use was first formalized by the International Union of Pure and Applied Chemistry (IUPAC) in 1970. This universally...
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Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar. Nucleic acid structure...
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Denaturation (biochemistry) (redirect from Nucleic acid denaturation)
secondary, and/or tertiary, and/or quaternary structures of proteins or nucleic acids resulting in a loss of bioactivity. Note 1: Modified from the definition...
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A locked nucleic acid (LNA), also known as bridged nucleic acid (BNA), and often referred to as inaccessible RNA, is a modified RNA nucleotide in which...
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Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid oligomers have been used in recent...
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Solid-phase reversible immobilization (category Nucleic acids)
is a method of purifying nucleic acids from solution. It uses silica- or carboxyl-coated paramagnetic beads, which reversibly bind to nucleic acids in the...
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Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature...
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Nucleic acid secondary structure is the basepairing interactions within a single nucleic acid polymer or between two polymers. It can be represented as...
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RNA (redirect from RiboNucleic Acid)
proteins (messenger RNA). RNA and deoxyribonucleic acid (DNA) are nucleic acids. The nucleic acids constitute one of the four major macromolecules essential...
65 KB (7,626 words) - 20:44, 25 May 2025
nucleic acids include peptide nucleic acids (PNA), morpholino, and locked nucleic acids (LNA), as well as glycol nucleic acids (GNA), threose nucleic...
49 KB (5,299 words) - 16:59, 25 May 2025
Nucleic acid NMR is the use of nuclear magnetic resonance spectroscopy to obtain information about the structure and dynamics of nucleic acid molecules...
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Nucleic acid design is the process of generating a set of nucleic acid base sequences that will associate into a desired conformation. Nucleic acid design...
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method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is...
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A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species...
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Xenonucleic acids (XNAs) are synthetic nucleic acid analogues that are engineered with structurally distinct components, such as alternative nucleosides...
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Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural five-carbon ribose sugar found in RNA has been replaced by an unnatural...
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of nucleic acids". Annual Review of Biochemistry. 36: 407–48. doi:10.1146/annurev.bi.36.070167.002203. PMID 18257727. McClean, Phillip. "Nucleic Acid Hybridizations"...
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Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its sequence. Secondary structure...
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Polymerase chain reaction (redirect from Nucleic Acid Amplification)
in nucleic acid tests for the diagnosis of infectious diseases. PCR amplifies a specific region of a DNA strand (the DNA target). Most PCR methods amplify...
90 KB (11,293 words) - 13:08, 23 May 2025
structure of nucleic acids, such as RNA and DNA, can be largely classified into biophysical and biochemical methods. Biophysical methods use the fundamental...
35 KB (4,459 words) - 01:21, 3 December 2024
foaming and ensure deactivation of RNases. Column-based nucleic acid purification Nucleic acid methods Ethanol precipitation DNA separation by silica adsorption...
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NASBA (molecular biology) (redirect from Nucleic acid sequence-based amplification)
Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of...
13 KB (1,634 words) - 00:53, 27 May 2025
Books. Pollegioni L, Servi S, eds. (2012). Unnatural Amino Acids: Methods and Protocols. Methods in Molecular Biology. Vol. 794. Humana Press. p. v. doi:10...
94 KB (10,035 words) - 12:47, 25 May 2025
The Warburg–Christian method is an ultraviolet spectroscopic protein and nucleic acid assay method based on the absorbance of UV light at 260 nm and 280...
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