The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...
90 KB (11,293 words) - 13:08, 23 May 2025
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called...
45 KB (5,784 words) - 22:04, 29 May 2025
Nested polymerase chain reaction (nested PCR) is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to...
3 KB (361 words) - 16:20, 23 October 2023
real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain...
43 KB (5,360 words) - 23:36, 25 May 2025
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods...
70 KB (7,425 words) - 17:07, 24 May 2025
The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying...
3 KB (412 words) - 08:32, 26 July 2024
Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence...
3 KB (325 words) - 18:27, 11 December 2023
which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). PCR inhibition is the most common cause of amplification...
5 KB (538 words) - 15:57, 19 October 2024
The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have...
16 KB (2,030 words) - 10:07, 20 June 2024
terms and components used in the PCR process.) The history of the polymerase chain reaction (PCR) has variously been described as a classic "Eureka!" moment...
23 KB (3,101 words) - 17:04, 25 May 2025
Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously...
7 KB (763 words) - 14:38, 12 February 2025
often abbreviated to Taq or Taq pol. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments...
22 KB (2,627 words) - 14:06, 26 May 2025
Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) amplification that relies...
4 KB (441 words) - 20:07, 27 November 2023
The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by...
8 KB (1,052 words) - 15:31, 26 May 2025
DNA replication (redirect from Leading chain)
at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction (LCR), and transcription-mediated amplification...
61 KB (7,438 words) - 12:39, 30 May 2025
biochemist. In recognition of his role in the invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with...
48 KB (4,890 words) - 16:33, 24 May 2025
replication. A DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) (PDB 1BGX, EC 2.7.7.7) is used in the polymerase chain reaction, an important...
7 KB (673 words) - 23:08, 9 July 2024
Primer (molecular biology) (category Polymerase chain reaction)
that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable...
16 KB (2,112 words) - 01:32, 8 September 2024
Reverse complement polymerase chain reaction (RC-PCR) is a modification of the polymerase chain reaction (PCR). It is primarily used to generate amplicon...
14 KB (1,544 words) - 21:08, 23 May 2025
biological research labs for a variety of applications. PCR, or Polymerase Chain Reaction, is a widely used molecular biology technique to amplify a specific...
104 KB (11,520 words) - 00:57, 29 May 2025
Lactobacillus delbrueckii subsp. bulgaricus (section Quantification in cow's milk cheese via real-time polymerase chain reaction assay)
2017, there was a study involving the development of a real-time polymerase chain reaction (qPCR) assay for quantifying Lactobacillus delbrueckii subsp....
23 KB (2,490 words) - 07:50, 27 March 2025
Thermus aquaticus (category Polymerase chain reaction)
enzyme Taq DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification...
15 KB (1,802 words) - 22:27, 5 February 2025
Molecular biology (section Polymerase chain reaction)
DNA sequence coding for a protein of interest is cloned using polymerase chain reaction (PCR), and/or restriction enzymes, into a plasmid (expression...
49 KB (5,554 words) - 06:34, 26 May 2025
uses a new RNA primer for each Okazaki fragment it comes across. Polymerase chain reaction (PCR) is a method used in laboratories that significantly increases...
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Myxomatosis (section Polymerase chain reaction)
development of molecular methods such as polymerase chain reaction (PCR) and real-time polymerase chain reaction assays has created faster and more accurate...
42 KB (4,986 words) - 22:12, 15 May 2025
amplification, digital polymerase chain reaction, microarray analysis, and next-generation sequencing. Polymerase chain reaction (PCR) is a process that...
181 KB (20,997 words) - 20:28, 29 May 2025
are used for the polymerase chain reaction and related methods for the amplification and modification of DNA. Several DNA polymerases have been described...
41 KB (5,192 words) - 18:26, 29 November 2024
Reverse transcriptase (redirect from RNA-directed DNA polymerase)
sequence of reactions is widely used in the laboratory to convert RNA to DNA for use in molecular cloning, RNA sequencing, polymerase chain reaction (PCR),...
27 KB (3,042 words) - 15:04, 8 May 2025
DNA synthesis (section Polymerase chain reaction)
DNA replication - DNA biosynthesis (in vivo DNA amplification), polymerase chain reaction - enzymatic DNA synthesis (in vitro DNA amplification) or gene...
14 KB (1,820 words) - 18:46, 3 May 2025
Bisulfite sequencing (redirect from Methylation-specific polymerase chain reaction)
methylation-specific PCR (MSP) (Figure 4), and strategies employing polymerase chain reaction (PCR) performed under non-methylation-specific conditions (Figure...
35 KB (4,227 words) - 13:17, 23 May 2025