Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is...
11 KB (1,337 words) - 06:50, 3 March 2023
Structured illumination light sheet microscopy (category Optical imaging)
was developed to allow for fine optical sectioning of thick biological samples without the need for physical sectioning or clearing, which are both time...
21 KB (2,784 words) - 03:19, 26 August 2024
a well-known problem that prevents optical sectioning using a wide-field microscope. The two-dimensional optical transfer function at the focal plane...
43 KB (5,652 words) - 18:18, 14 December 2024
Confocal microscopy (redirect from Scanning confocal optical microscopy)
reconstruction of three-dimensional structures (a process known as optical sectioning) within an object. This technique is used extensively in the scientific...
46 KB (5,308 words) - 02:06, 5 November 2024
achieved by adopting an optics/data-processing co-design scheme. Optical sectioning, also known as sectional image reconstruction, is the process of recovering...
14 KB (1,461 words) - 07:30, 23 April 2025
Light sheet fluorescence microscopy (category Optical microscopy techniques)
microscopy technique with an intermediate-to-high optical resolution, but good optical sectioning capabilities and high speed. In contrast to epifluorescence...
42 KB (4,675 words) - 12:57, 30 March 2025
Optical cross section (OCS) is a value which describes the maximum amount of optical flux reflected back to the source. The standard unit of measurement...
2 KB (332 words) - 07:44, 19 May 2024
Microscopy (category Optical microscopy)
shift as the focus is changed. This limitation makes techniques like optical sectioning or accurate measurement on the z-axis impossible. Dark field microscopy...
69 KB (8,316 words) - 20:02, 23 April 2025
Two-photon excitation microscopy (redirect from Nonlinear optical microscopy)
pinhole apertures that give confocal microscopes their optical sectioning quality. The optical sectioning produced by multiphoton microscopes is a result of...
35 KB (3,552 words) - 00:29, 2 April 2025
corresponding to the shadow. The technique is particularly suitable for optical sectioning at lower magnifications. An example of the use of HMC illumination...
3 KB (297 words) - 03:18, 4 April 2023
Fluorescence microscope (category Optical microscopy techniques)
more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. The specimen is...
25 KB (2,725 words) - 05:14, 1 April 2025
techniques such as immunolabeling and subsequently imaged, most often by optical sectioning microscopy techniques. Tissue clearing has been applied to many areas...
19 KB (2,064 words) - 03:41, 4 January 2025
An optical fiber, or optical fibre, is a flexible glass or plastic fiber that can transmit light from one end to the other. Such fibers find wide usage...
100 KB (12,214 words) - 18:00, 4 May 2025
Synchronous Optical Networking (SONET) and Synchronous Digital Hierarchy (SDH) are standardized protocols that transfer multiple digital bit streams synchronously...
45 KB (5,525 words) - 23:48, 9 March 2025
Optical holography is a technique which enables an optical wavefront to be recorded and later re-constructed. Holography is best known as a method of generating...
39 KB (5,886 words) - 13:07, 22 March 2025
Photonic integrated circuit (redirect from Integrated optical circuit)
A photonic integrated circuit (PIC) or integrated optical circuit is a microchip containing two or more photonic components that form a functioning circuit...
26 KB (2,963 words) - 07:30, 7 April 2025
Slit lamp (category Optical devices)
sometimes considered an illumination technique. Observation with an optical section or direct focal illumination is the most frequently applied method...
20 KB (2,550 words) - 03:27, 24 April 2025
fiber-optic communication, a single-mode optical fiber (SMF), also known as fundamental- or mono-mode, is an optical fiber designed to carry only a single...
14 KB (1,713 words) - 12:57, 2 May 2025
blurred, out-of-focus background signal. Endomicroscopes achieve optical sectioning (removal of the background intensity) using the confocal principle...
12 KB (1,703 words) - 01:47, 11 May 2024
camera or CCD camera. Confocal and two-photon microscopes provide optical sectioning ability so that calcium signals can be resolved in microdomains such...
35 KB (3,885 words) - 19:44, 15 October 2024
Microtome (redirect from Serial sectioning)
areas of interest under a light microscope prior to thin sectioning for the TEM. Thin sectioning for the TEM is often done with a gem quality diamond knife...
26 KB (3,303 words) - 17:25, 17 December 2024
An optical cavity, resonating cavity or optical resonator is an arrangement of mirrors or other optical elements that confines light waves similarly to...
17 KB (2,250 words) - 17:43, 23 April 2025
An optical disc is a flat, usually disc-shaped object that stores information in the form of physical variations on its surface that can be read with...
68 KB (6,997 words) - 16:34, 12 February 2025
Korber DR, Hoyle BD, Costerton JW, Caldwell DE (October 1991). "Optical sectioning of microbial biofilms". Journal of Bacteriology. 173 (20): 6558–67...
121 KB (14,150 words) - 03:59, 5 May 2025
Laser (redirect from Optical maser)
A laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation. The word...
126 KB (13,996 words) - 18:30, 22 April 2025
Optical character recognition or optical character reader (OCR) is the electronic or mechanical conversion of images of typed, handwritten or printed text...
36 KB (4,093 words) - 10:19, 21 March 2025
Escobar, Isabel; Saavedra, Genaro; Martinez-Corral, Manuel (2004). "Optical-sectioning improvement in two-color excitation scanning microscopy". Microscopy...
16 KB (2,064 words) - 19:39, 8 August 2023
from the acoustic detection. This allows this technology to have optical sectioning capability. For point targets, the achievable axial resolution is...
27 KB (4,140 words) - 21:18, 21 July 2023
Piscataway, New Jersey, where he published four patent applications on optical sectioning, Line Artifact Removal, Brightfield image segmentation and Cell Tracking...
5 KB (547 words) - 19:11, 1 May 2025
believed[by whom?] that the concept of moving an optical fiber to produce 2D images with confocal sectioning and laser illumination was first proposed for...
9 KB (1,316 words) - 03:39, 1 July 2024