• Thumbnail for Two-photon excitation microscopy
    fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons...
    33 KB (3,401 words) - 00:41, 31 May 2025
  • It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The fluorescence lifetime...
    24 KB (3,092 words) - 21:09, 11 July 2024
  • Thumbnail for Microscopy
    number of techniques labeled as nonlinear microscopy, saturation microscopy, and two-photon excitation microscopy. High-intensity, short-pulse laboratory...
    69 KB (8,316 words) - 00:38, 31 May 2025
  • Three-photon microscopy (3PEF) is a high-resolution fluorescence microscopy based on nonlinear excitation effect. Different from two-photon excitation microscopy...
    17 KB (2,109 words) - 00:43, 31 May 2025
  • Thumbnail for Two-photon absorption
    physics, two-photon absorption (TPA or 2PA), also called two-photon excitation or non-linear absorption, is the (almost) simultaneous absorption of two photons...
    44 KB (5,529 words) - 05:46, 31 May 2025
  • two-photon excitation microscopy). Moreover, these photons cause less damage to the sample, since they are of lower energy. In some cases, two energy transitions...
    104 KB (11,278 words) - 06:07, 23 May 2025
  • Multi-photon microscopy (also spelled multiphoton microscopy) may refer to: Two-photon excitation microscopy Three photon microscopy Second-harmonic imaging...
    364 bytes (66 words) - 09:40, 10 May 2020
  • microscope Super-resolution microscopy Total internal reflection fluorescence microscope (TIRF) Two-photon excitation microscopy Pawley JB, ed. (2006). Handbook...
    46 KB (5,308 words) - 00:47, 31 May 2025
  • Thumbnail for Fluorescence microscope
    4Pi microscopy maximizes the amount of available focusing directions by using two opposing objective lenses or two-photon excitation microscopy using...
    25 KB (2,725 words) - 05:14, 1 April 2025
  • Thermomechanical analysis TOF-MS – Time-of-flight mass spectrometry Two-photon excitation microscopy TXRF – Total reflection X-ray fluorescence analysis Ultrasound...
    15 KB (1,398 words) - 20:21, 9 November 2024
  • Thumbnail for Non-degenerate two-photon absorption
    non-degenerate two-photon absorption (ND-TPA or ND-2PA) or two-color two-photon excitation is a type of two-photon absorption (TPA) where two photons with different...
    16 KB (2,064 words) - 19:39, 8 August 2023
  • Chromosome 2 (human) 2p, an atomic orbital for electrons Two-photon absorption Two-photon excitation microscopy Airphil Express, a Philippines airline, IATA code...
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  • Thumbnail for STED microscopy
    occur either from excitation into an even higher excited state, or from excitation in the triplet state. To prevent the excitation of an excited electron...
    33 KB (3,867 words) - 00:41, 31 May 2025
  • Thumbnail for Fluo-3
    derivatives (Fluo-4, Fluo-5 etc) have also been widely used with two-photon excitation microscopy. Fluo-3 is an essentially nonfluorescent compound, but upon...
    4 KB (296 words) - 19:21, 30 November 2023
  • Thumbnail for Second-harmonic imaging microscopy
    anti-Stokes Raman Scattering or Two-photon excitation microscopy, as part of a routine called multiphoton microscopy (or tomography) that provides a non-invasive...
    48 KB (5,024 words) - 00:48, 31 May 2025
  • employed in the context of optical microscopy, in particular confocal microscopy or two-photon excitation microscopy. In these techniques light is focused...
    63 KB (7,969 words) - 21:02, 28 May 2025
  • Two-photon microscopy remains the only technique that allows the recording of activity in living brains with high spatial resolution. 2p excitation can...
    18 KB (1,710 words) - 19:07, 22 May 2025
  • PMID 15128753. Svoboda K, Yasuda R (June 2006). "Principles of two-photon excitation microscopy and its applications to neuroscience". Neuron. 50 (6): 823–839...
    34 KB (3,823 words) - 17:42, 21 May 2025
  • lobes in the point spread function. Typically (but not always) two-photon excitation microscopy is used in a 4Pi microscope in combination with an emission...
    9 KB (1,149 words) - 09:41, 14 July 2024
  • Svoboda, Karel; Yasuda, Ryohei (2006-06-15). "Principles of two-photon excitation microscopy and its applications to neuroscience". Neuron. 50 (6): 823–839...
    38 KB (4,383 words) - 19:57, 25 May 2025
  • confocal microscopy (also known as single-photon microscopy) and two-photon microscopy. Three-photon excitation microscopy (3PEF) was first performed in 1964...
    10 KB (1,174 words) - 21:18, 26 May 2025
  • Thumbnail for Electrical brain stimulation
    pathologic aggression and rage with stimulation of the amygdala. Two-photon excitation microscopy has shown that microstimulation activates neurons sparsely...
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  • the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field...
    89 KB (10,346 words) - 00:41, 31 May 2025
  • undergo both two-photon excitation and fluorescence. Pump–probe microscopy circumvents this limitation by directly measuring excitation light. This expands...
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  • development of electron scanning tunneling microscopy in 1982 by Binning et al., this led to the development of the photon scanning tunneling microscope by Reddick...
    26 KB (3,555 words) - 19:57, 28 December 2023
  • emitted from calcium indicators expressed in neurons. However, two-photon excitation microscopy is slow, because it has to scan all the pixels one by one in...
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  • Thumbnail for List of laser applications
    charged ions to high energies. Confocal laser scanning microscopy and Two-photon excitation microscopy make use of lasers to obtain blur-free images of thick...
    24 KB (2,749 words) - 06:01, 17 March 2025
  • Minflux (category Microscopy)
    minimal fluorescence photon fluxes microscopy, is a super-resolution light microscopy method that images and tracks objects in two and three dimensions...
    17 KB (1,842 words) - 22:53, 24 May 2025
  • electron excitation in the material. Here, a first pulse in the visible excites dynamics near the sample surface and a second pulse with a photon energy...
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  • Thumbnail for Characterization (materials science)
    of microscopy techniques include: Optical microscopy Scanning electron microscopy (SEM) Transmission electron microscopy (TEM) Field ion microscopy (FIM)...
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