• Thumbnail for Real-time polymerase chain reaction
    (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...
    43 KB (5,360 words) - 20:54, 17 February 2025
  • Thumbnail for Reverse transcription polymerase chain reaction
    real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will...
    45 KB (5,776 words) - 17:52, 21 March 2025
  • Thumbnail for Quantitative PCR instrument
    A quantitative PCR instrument, also called real-time PCR machine, is an analytical instrument that amplifies and detects DNA. It combines the functions...
    11 KB (1,110 words) - 05:13, 23 May 2025
  • Thumbnail for Polymerase chain reaction
    RT-PCR (real-time PCR) but this abbreviation should be used only for reverse transcription PCR. qPCR is the appropriate contractions for quantitative PCR...
    90 KB (11,293 words) - 13:08, 23 May 2025
  • Thumbnail for RNase H-dependent PCR
    PCR reaction. These primers can be used in both 5’ nuclease Taqman and SYBR Green types of quantitative PCR. rhPCR can be used for quantitative PCR and...
    5 KB (596 words) - 01:29, 3 December 2023
  • Thumbnail for Bisulfite sequencing
    high-resolution melting analysis (HRM), a quantitative PCR-based technique initially designed to distinguish SNPs. The PCR amplicons are analyzed directly by...
    35 KB (4,227 words) - 13:17, 23 May 2025
  • Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
    20 KB (2,673 words) - 22:47, 23 May 2025
  • Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction...
    70 KB (7,425 words) - 17:07, 24 May 2025
  • hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher David Gefland...
    6 KB (684 words) - 21:36, 10 May 2025
  • advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
    38 KB (5,658 words) - 06:18, 10 April 2025
  • Thumbnail for Aneuploidy
    hybridization (FISH), quantitative PCR of short tandem repeats, quantitative fluorescence PCR (QF-PCR), quantitative PCR dosage analysis, Quantitative Mass Spectrometry...
    37 KB (3,586 words) - 11:07, 11 April 2025
  • Thermal cycler (redirect from PCR machine)
    and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
    7 KB (556 words) - 21:34, 19 February 2025
  • 2003). "Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the...
    3 KB (334 words) - 13:39, 10 March 2025
  • of interest to a broad readership. The journal was established in 1991 as PCR Methods and Applications and obtained its current title in 1995. According...
    3 KB (190 words) - 10:49, 24 June 2024
  • PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
    7 KB (763 words) - 14:38, 12 February 2025
  • Thumbnail for Kompetitive allele specific PCR
    Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific...
    7 KB (988 words) - 10:29, 10 January 2024
  • competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification. In quantitative PCR, PDs may interfere...
    14 KB (1,578 words) - 22:08, 23 December 2024
  • product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
    33 KB (4,155 words) - 13:49, 10 March 2025
  • Thumbnail for Human herpesvirus 6
    viral DNA in blood, other body fluids, and organs by means of real-time PCR. The classical presentation of primary HHV-6b infection is as exanthema subitum...
    78 KB (8,118 words) - 14:26, 26 April 2025
  • Thumbnail for In silico PCR
    multiplex PCR, allowing for an estimate of quantitative competition effects between multiple amplicons in one reaction. The UCSC Genome Browser offers isPCR, which...
    8 KB (867 words) - 01:06, 25 December 2024
  • Thumbnail for Combined bisulfite restriction analysis
    restriction enzyme digestion followed by PCR. Quantitative: This is in contrast to methylation-specific PCR, which is qualitative. With COBRA, DNA methylation...
    12 KB (1,300 words) - 09:49, 24 April 2025
  • Thumbnail for Kary Mullis
    reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a...
    48 KB (4,890 words) - 16:33, 24 May 2025
  • Thumbnail for Forensic biology
    form of the polymerase chain reaction, known as real-time PCR, quantitative PCR (qPCR). qPCR is the preferred method of DNA quantitation for forensic cases...
    52 KB (6,017 words) - 20:14, 6 March 2025
  • down a station Quantitative PCR or qrt-PCR, a laboratory DNA detection technique Qube Research & Technologies, London-based quantitative investment management...
    581 bytes (121 words) - 04:48, 12 February 2024
  • quantitative real time PCR.[citation needed] cffDNA may be detected by finding paternally inherited DNA sequences via polymerase chain reaction (PCR)...
    51 KB (5,957 words) - 22:26, 22 May 2025
  • Thumbnail for Mikael Kubista
    the Czech Republic. Kubista has contributed to the field of quantitative real-time PCR (qPCR), with his work recognized as part of the early research in...
    23 KB (2,031 words) - 22:11, 23 May 2025
  • sequence for the gene. Quantitative PCR (Q-PCR) is used to measure the quantity of a PCR product (preferably real-time, QRT-PCR). It is the method of choice...
    10 KB (1,401 words) - 12:18, 27 July 2024
  • Thumbnail for COVID-19 testing
    as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum...
    181 KB (21,007 words) - 23:32, 22 May 2025
  • DNA molecule. These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification. Applications...
    2 KB (230 words) - 12:51, 27 April 2024
  • Thumbnail for Psittacine beak and feather disease
    presence of BFDV are available: standard polymerase chain reaction (PCR), quantitative PCR (qPCR) which can detect the virus in extremely small quantities, whole-genome...
    40 KB (4,438 words) - 01:51, 7 May 2025