debate. CRISPR activation Anti-CRISPR CRISPR/Cas Tools CRISPR gene editing The CRISPR Journal "Designer baby" DRACO Gene knockout Genome-wide CRISPR-Cas9...
115 KB (11,901 words) - 05:05, 23 May 2025
Chemistry, "for the development of a method for genome editing." Biology portal Technology portal CRISPR/Cas Tools The CRISPR Journal Eugenics DRACO Zinc finger...
176 KB (20,515 words) - 05:53, 27 May 2025
(gRNAs) for use with the CRISPR/Cas gene editing system. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated nucleases)...
16 KB (1,283 words) - 21:43, 2 January 2025
irradiation or by the CRISPR/CAS method. Similarly, mutants of A. sojae with altered properties were generated by a variant of the CRISPR/Cas method or chemical...
28 KB (3,685 words) - 13:39, 26 May 2025
Cas9 (redirect from CRISPR/Cas9)
have evolved methods to evade and fend off predatory viruses. This includes the CRISPR system of adaptive immunity. In practice, CRISPR/Cas systems act...
59 KB (7,257 words) - 05:11, 23 May 2025
Human genetic enhancement (section CRISPR/Cas9)
genetic disorders. Gene treatment employing the CRISPR/Cas genome editing method is known as CRISPR/Cas-based gene therapy. Mammalian cells can be genetically...
93 KB (10,228 words) - 20:00, 16 May 2025
adaptive immune system which incorporates CRISPR RNA (crRNA) and CRISPR-associated (cas) genes. The CRISPR interference (CRISPRi) technique was first...
24 KB (2,898 words) - 12:08, 22 March 2024
Cas12a (redirect from CRISPR/Cas12a)
the host cell from infection. Like other Cas enzymes, Cas12a binds to a "guide" RNA (termed a crRNA, or CRISPR RNA) which targets it to a DNA sequence...
22 KB (2,638 words) - 03:05, 25 May 2025
CRISPR activation (CRISPRa) is a gene regulation technique that utilizes an engineered form of the CRISPR-Cas9 system to enhance the expression of specific...
22 KB (2,739 words) - 08:03, 1 April 2025
in phages, that inhibit the normal activity of CRISPR-Cas, the immune system of certain bacteria. CRISPR consists of genomic sequences that can be found...
32 KB (3,690 words) - 10:49, 25 May 2025
Guide RNA (section CRISPR In Prokaryotes)
Cas-proteins that cut the double-stranded DNA and thereby can be used for gene editing. In bacteria and archaea, gRNAs are a part of the CRISPR-Cas system...
30 KB (3,644 words) - 14:03, 23 May 2025
enzyme CRISPR-associated (Cas) proteins, involved in the prokaryotic immune system and genome editing CAS parameters, an image analysis method typically...
7 KB (843 words) - 13:25, 16 February 2025
Genetic screen (section CRISPR)
patterning that mimic loss of wingless function. CRISPR/Cas is primarily used for reverse genetic screens. CRISPR has the ability to create libraries of thousands...
18 KB (2,199 words) - 17:53, 21 April 2024
Philippe; Magadán, Alfonso H.; Moineau, Sylvain (November 4, 2010). "The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA". Nature...
27 KB (2,302 words) - 15:08, 28 May 2025
Genome editing (section CRISPR)
engineered meganucleases—were selected by Nature Methods as the 2011 Method of the Year. The CRISPR-Cas system was selected by Science as 2015 Breakthrough...
79 KB (9,365 words) - 19:52, 22 May 2025
Gene knockdown (section CRISPRs)
or CRISPRs. CRISPR-associated (cas) genes encode cellular machinery that cuts exogenous DNA into small fragments and inserts them into a CRISPR repeat...
13 KB (1,611 words) - 03:43, 8 April 2025
February 19, 1964) is an American biochemist who has pioneered work in CRISPR gene editing, and made other fundamental contributions in biochemistry and...
69 KB (5,926 words) - 19:03, 25 May 2025
Francisco Mojica (section The discovery of CRISPR)
Heroes of CRISPR) Mojica, Francisco J. M.; Garrett, Roger A. (13 December 2012). "Discovery and Seminal Developments in the CRISPR Field". CRISPR-Cas Systems...
20 KB (1,955 words) - 15:49, 15 October 2023
TIGR-Tas (section Advantages over CRISPR)
that utilize a unique dual-spacer targeting mechanism, distinct from CRISPR-Cas systems. TIGR-Tas systems were reported in February 2025 by researchers...
10 KB (1,006 words) - 09:40, 24 May 2025
He Jiankui affair (redirect from CRISPR baby)
"One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering". Cell. 154 (6): 1370–1379. doi:10.1016/j...
120 KB (12,426 words) - 15:33, 30 April 2025
"Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system". Cell. S0092-8674 (18): 31185–1. doi:10.1016/j.cell.2013.12.001...
8 KB (556 words) - 04:46, 2 July 2023
more complicated method of such as Golden gate cloning. In 2011, another major breakthrough technology was developed based on CRISPR/Cas (clustered regularly...
55 KB (6,765 words) - 11:43, 11 December 2024
alternatives that can limit the off-target burden when possible. CRISPR-cas13b, using a type IV CRISPR-Cas system (as opposed to the commonly used type II) can target...
55 KB (7,122 words) - 04:03, 28 April 2025
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
55 KB (6,172 words) - 00:49, 29 May 2025
escape.. Faure has made significant contributions to the understanding of CRISPR-Cas systems and their association with mobile genetic elements (MGEs). His...
11 KB (1,157 words) - 21:33, 27 May 2025
Designer baby (section CRISPR/Cas9)
trait. Other methods of altering a baby's genetic information involve directly editing the genome before birth, using technologies such as CRISPR. A controversial...
78 KB (9,030 words) - 19:52, 22 May 2025
Emmanuelle Charpentier (section CRISPR/Cas9)
the Nobel Prize in Chemistry "for the development of a method for genome editing" (through CRISPR). This was the first science Nobel Prize ever won by two...
44 KB (3,368 words) - 16:23, 24 May 2025
while exerting no aberrant effects on wild-type alleles. CRISPR CRISPR/Cpf1 Addgene CRISPR-Cas Guide Shah SA, Erdmann S, Mojica FJ, Garrett RA (2013)....
12 KB (1,421 words) - 00:22, 14 December 2022
Gene targeting (section Methods)
engineered homing endonucleases, TALENS, or most commonly the CRISPR-Cas system. This method has been applied to species including Drosophila melanogaster...
40 KB (4,473 words) - 03:37, 8 April 2025
Gene knockout (section CRISPR/Cas9)
organism's genome. This can be done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantages of gene...
22 KB (2,563 words) - 23:23, 24 May 2025