Duplex sequencing is a library preparation and analysis method for next-generation sequencing (NGS) platforms that employs random tagging of double-stranded...

approach is 9 in 1 million. Duplex sequencing is an improvement on the single UIDs added in the Safe-Seq technique. In duplex sequencing, randomized double-stranded...

Whole genome sequencing (WGS), also known as full genome sequencing, complete genome sequencing, or entire genome sequencing, is the process of determining...

"Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease". Current Protocols in Molecular Biology...

radicals. Loeb's laboratory developed “Duplex Sequencing” technology, which permits high accuracy high throughput sequencing for the identification of low prevalence...

become the de facto standard for storing the output of high-throughput sequencing instruments such as the Illumina Genome Analyzer. A FASTQ file has four...

many other animals and in some it exists as two separate uteri known as a duplex uterus. In medicine and related professions, the term uterus is consistently...

media report high levels of '1D' accuracy (sequencing one strand of the duplex DNA), '2D' accuracy (sequencing both the template and complement strand)...

cloning and sequencing of the suspect gene. The amino acid sequence encoded by the gene is then predicted. NA Sequencing: Sanger sequencing or Next-Generation...

massively parallel DNA sequencing. An R-loop is a three-stranded nucleic acid structure, which consists of a DNA-RNA hybrid duplex and a displaced single...

In epitranscriptomic sequencing, most methods focus on either (1) enrichment and purification of the modified RNA molecules before running on the RNA sequencer...

reaction (PCR) and identified by virtue of their DNA code (e.g.by DNA sequencing). In evolution-based DEL technologies hits can be further enriched by...

they are selectively denatured at the Tc. Primer annealing stage. The homo-duplex DNA will preferentially remain double stranded and not be available for...

as the precursor to Sequenase, an engineered-enzyme optimized for DNA sequencing. Figure 2. Nucleotidyl transfer by DNA polymerase. T7 DNA polymerase catalyzes...

than a similar mismatch in a DNA/DNA duplex. This binding strength and specificity also applies to PNA/RNA duplexes. PNAs are not easily recognized by either...

form hybrid duplex molecules. scaffolding scRNA See small conditional RNA. second-generation sequencing See massively parallel sequencing. selectable...

(2013). "Partial Base Flipping is Sufficient for Strand Slippage near DNA Duplex Termini". Journal of the American Chemical Society. 135 (22): 8274–8282...

methylated cytosines will not be affected. Certain sequencing methods, such as nanopore sequencing, allow sequencing of native DNA. Native (=unamplified) DNA retains...

and DNA can be used for a variety of applications, such as genotyping, sequencing, and detection of bacteria or viruses. There are two different types of...

naturally-encoded 20 amino acids, and provides insight into how nucleic acids form duplex structures, how proteins interact with nucleic acids, and how alternative...

sequence with traditional and high throughput DNA sequencing methods (see Sanger sequencing and DNA sequencing). However, these methods provide large amounts...

or NewTek's Video Toaster, thus providing a complete package of MIDI sequencing and/or video synchronization with non-linear hard disk recording. In 1993...

approximately 8 nm long. The genome was first sequenced in 1986. It is a linear duplex DNA molecule, a laboratory strain has 124,884 base pairs. The genome has...

can vary widely between Taqs, which has profound effects in downstream sequencing applications. Taq makes DNA products that have A (adenine) overhangs at...

phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically...

result of a single stranded RNA forming hydrogen bonds with an RNA duplex; the duplex consists of Watson-Crick base pairing while the third strand binds...

enables the melting of the duplex DNA to unmask the PQS, adopting a G-quadruplex fold. With the use of genome-wide ChIP-sequencing analyses, cell-based assays...

Al-Hashimi HM (2013). "A historical account of Hoogsteen base-pairs in duplex DNA". Biopolymers. 99 (12): 955–68. doi:10.1002/bip.22334. PMC 3844552....

the laboratory to convert RNA to DNA for use in molecular cloning, RNA sequencing, polymerase chain reaction (PCR), or genome analysis. Reverse transcriptases...

namely: It is cost-effective vs. other genotyping technologies such as sequencing and TaqMan SNP typing. This makes it ideal for large scale genotyping...