• Thumbnail for Firefly luciferase
    Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation...
    14 KB (1,608 words) - 13:16, 19 December 2023
  • Thumbnail for Luciferase
    different luciferases in a variety of light-emitting reactions. The majority of studied luciferases have been found in animals, including fireflies, and many...
    27 KB (2,896 words) - 13:08, 29 March 2024
  • Thumbnail for Firefly
    Firefly luciferase is used in forensics, and the enzyme has medical uses – in particular, for detecting the presence of ATP or magnesium. Fireflies produce...
    41 KB (3,946 words) - 08:26, 6 April 2024
  • Thumbnail for Firefly luciferin
    is the substrate of luciferase (EC 1.13.12.7), which is responsible for the characteristic yellow light emission from many firefly species. As with all...
    10 KB (960 words) - 13:35, 23 February 2024
  • Thumbnail for Photinus pyralis
    the firefly (Photinus pyralis). The Biological Bulletin, 375-393 Keller, G.A.; Gould, S.; Deluca, M.; Subramani, S. (1987). "Firefly luciferase is targeted...
    29 KB (3,556 words) - 15:46, 27 April 2024
  • Thumbnail for Bioluminescence
    luciferase, respectively. Because these are generic names, luciferins and luciferases are often distinguished by the species or group, e.g. firefly luciferin...
    74 KB (8,010 words) - 07:41, 9 May 2024
  • Thumbnail for Bioreporter
    of toxicity. Firefly luciferase catalyzes a reaction that produces visible light in the 550 to 575 nm range. A click-beetle luciferase is also available...
    14 KB (1,843 words) - 05:47, 1 May 2024
  • Thumbnail for Bioluminescence imaging
    organisms which bioluminesce, also known as luciferase enzymes. The three main sources are the North American firefly, the sea pansy (and related marine organisms)...
    14 KB (1,585 words) - 18:32, 26 March 2024
  • detection using the firefly luciferase enzyme. A mixture of three enzymes (DNA polymerase, ATP sulfurylase and firefly luciferase) and a nucleotide (dNTP)...
    9 KB (1,110 words) - 17:05, 14 June 2023
  • Thumbnail for Photinini
    Photinini are a large tribe of fireflies in the subfamily Lampyrinae. Photinus pyralis is famous in biotechnology for its luciferase gene. This is sometimes...
    4 KB (254 words) - 06:58, 3 March 2023
  • Thumbnail for Peroxisome
    used morphological term “microbodies”. Later, it was described that firefly luciferase is targeted to peroxisomes in mammalian cells, allowing the discovery...
    33 KB (3,863 words) - 12:56, 23 April 2024
  • Thumbnail for Firefly squid
    and the necessary enzyme luciferase are located in a crystalline structure within rod-like bodies in their photophores. Firefly squid are the only cephalopods...
    24 KB (2,725 words) - 19:01, 7 April 2024
  • Thumbnail for Circadian rhythm
    followed by 3 days of constant light (from 96h). Their genomes carry firefly luciferase reporter genes driven by the promoter sequences of clock genes. The...
    110 KB (12,903 words) - 21:50, 18 April 2024
  • of firefly has been determined to be due to differences in the amino acid sequences of the luciferases expressed in their photocytes. Two luciferase genes...
    29 KB (3,944 words) - 01:52, 5 May 2024
  • Thumbnail for Tricine
    the most effective buffer among the ten tested for ATP assays using firefly luciferase. Tricine has also been found to be an effective scavenger of hydroxyl...
    3 KB (309 words) - 17:31, 17 September 2023
  • research that led to the discovery of this consensus observed that when firefly luciferase was expressed in cultured insect cells it was targeted to the peroxisome...
    52 KB (6,394 words) - 03:48, 19 March 2024
  • Thumbnail for Entamoeba
    "Establishment of a transient transfection system and expression of firefly luciferase in Entamoeba invadens". Molecular and Biochemical Parasitology. 183...
    14 KB (1,482 words) - 20:12, 16 October 2023
  • Thumbnail for Reporter gene
    and chemical biology. Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the interpretation...
    17 KB (1,980 words) - 11:31, 24 March 2024
  • activity (proof-reading) and luminescence real-time detection using the firefly luciferase. All the key concepts of sequencing by synthesis were introduced,...
    29 KB (3,019 words) - 01:30, 11 December 2023
  • first purified firefly luciferin, which gives off light after being combined with ATP, magnesium ion, oxygen, and firefly luciferase. In 1956, he switched...
    2 KB (140 words) - 00:57, 6 May 2024
  • Thumbnail for Viability assay
    viability of cells using the assessment of ATP and a method known as "firefly luciferase". Sodium-potassium ratio: Another kind of assay practices the examination...
    12 KB (1,372 words) - 05:21, 8 February 2024
  • Thumbnail for Luciferin
    Luciferin (section Firefly)
    evolution. Firefly luciferin is the luciferin found in many Lampyridae species, such as P. pyralis. It is the substrate of beetle luciferases (EC 1.13.12...
    9 KB (867 words) - 20:20, 25 April 2024
  • Thumbnail for Ataluren
    biological target of ataluren, it was discovered to bind and stabilize firefly luciferase, thus explaining the mechanism by which it created a false positive...
    20 KB (1,637 words) - 13:24, 25 April 2024
  • forward genetic screen in which he linked a bioluminescent reporter, firefly (luciferase), to expression of CAB (chlorophyll-a,b binding protein—see Light-harvesting...
    14 KB (1,625 words) - 17:43, 29 January 2024
  • Fan, F.; et al. (2008). "Novel Genetically Encoded Biosensors Using Firefly Luciferase". ACS Chem. Biol. 3 (6): 346–51. doi:10.1021/cb8000414. PMID 18570354...
    104 KB (12,037 words) - 06:14, 15 April 2024
  • Thumbnail for Bacteriological water analysis
    produced through its reaction with the naturally occurring enzyme firefly luciferase using a luminometer. The amount of light produced is directly proportional...
    13 KB (1,754 words) - 10:40, 29 February 2024
  • Thumbnail for Norman Sharpless
    'glows' upon activation of the p16INK4a promoter due to insertion of firefly luciferase in place of the endogenous gene. Use of this system revealed the activation...
    21 KB (2,080 words) - 10:01, 16 March 2024
  • Thumbnail for MTOR
    "Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals". Proceedings...
    120 KB (12,710 words) - 11:01, 11 April 2024
  • Thumbnail for Agrobacterium tumefaciens
    into eukaryotic cells. This process has been performed using the firefly luciferase gene to produce glowing plants. This luminescence has been a useful...
    38 KB (4,328 words) - 07:20, 21 April 2024
  • his use of luciferase reporters for the purpose of studying plant circadian biology. Millar began experimenting with the firefly luciferase reporter gene...
    10 KB (1,052 words) - 19:18, 18 November 2023