• Thumbnail for Fluorophore
    A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores...
    27 KB (2,024 words) - 06:28, 19 April 2024
  • Thumbnail for Immunofluorescence
    conjugating the antibody to a fluorophore, the position of the target biomolecule is visualized by exciting the fluorophore and measuring the emission of...
    21 KB (2,054 words) - 16:47, 21 April 2024
  • Thumbnail for Glow stick
    when the second fluorophore would degrade in solution or be attacked by the chemicals. The emission spectrum of the first fluorophore and the absorption...
    25 KB (2,784 words) - 09:52, 7 April 2024
  • consist of a fluorophore covalently attached to the 5’-end of the oligonucleotide probe and a quencher at the 3’-end. Several different fluorophores (e.g....
    6 KB (684 words) - 02:15, 23 December 2023
  • Thumbnail for Fluorescence
    The latter may be referred to as biofluorescence, indicating that the fluorophore is part of or is extracted from a living organism (rather than an inorganic...
    98 KB (10,883 words) - 23:05, 8 May 2024
  • Fluorophore assisted carbohydrate electrophoresis or FACE is a biochemical technology suited for detecting complex mixtures of high molecular weight N-glycans...
    1 KB (113 words) - 17:01, 20 June 2022
  • (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused...
    5 KB (582 words) - 20:35, 29 January 2024
  • Thumbnail for Fluorescence microscope
    light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different...
    25 KB (2,745 words) - 21:47, 19 April 2024
  • Thumbnail for Fluorescence spectroscopy
    from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers...
    22 KB (2,841 words) - 16:12, 30 April 2024
  • Thumbnail for Brainbow
    exposed to a photon with its particular excitation wavelength, each fluorophore emits a signal that is collected into a red, green, or blue channel,...
    15 KB (1,845 words) - 01:04, 28 February 2024
  • Thumbnail for Bimolecular fluorescence complementation
    will bring the fluorophores into close spatial proximity. If the fluorophores are oriented in a manner that exposes the fluorophores to one another,...
    46 KB (5,192 words) - 21:00, 23 August 2022
  • Thumbnail for Intersystem crossing
    or fluorophores, in order to image biological systems. Since fluorescence and phosphorescence are competitive methods of relaxation, a fluorophore that...
    11 KB (1,552 words) - 06:55, 16 December 2023
  • super-resolution: the most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, which can be exploited to enhance...
    87 KB (10,121 words) - 08:36, 16 April 2024
  • multinational engineering and construction firm. Fluorite, a class of minerals Fluorophore, a fluorescent chemical compound Flour (disambiguation) Fleur (disambiguation)...
    370 bytes (76 words) - 02:08, 12 April 2022
  • the probe is a fluorophore and to the other end a fluorescence quencher. Because of the stem-loop structure of the probe, the fluorophore is close to the...
    37 KB (5,005 words) - 06:12, 10 March 2024
  • Thumbnail for Activity-based proteomics
    an activity-based probe. The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with...
    5 KB (536 words) - 00:09, 24 March 2022
  • differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy...
    24 KB (3,093 words) - 05:25, 4 February 2024
  • negative results. This method involves two fluorophores: a donor and an acceptor. Excitation of the donor fluorophore (in this case, the lanthanide ion complex)...
    106 KB (10,221 words) - 09:25, 7 May 2024
  • of dyes are used for PALM, STORM and related techniques, both organic fluorophores and fluorescent proteins. Some are compatible with live cell imaging...
    27 KB (3,213 words) - 14:27, 6 December 2023
  • Thumbnail for Retinal ganglion cell
    through a fluorescence microscope at 50x magnification. The optic nerve was injected with a fluorophore, causing retinal ganglion cells to fluoresce....
    29 KB (3,522 words) - 10:37, 3 December 2023
  • Thumbnail for Optical brightener
    reflected by the material, with the blue and purple optical emission of the fluorophore. The most common classes of compounds with this property are the stilbenes...
    7 KB (839 words) - 13:47, 6 November 2022
  • between the observed fluorescence intensity and the distance of the fluorophore from a reflective silicon surface. The observed fluorescence intensity...
    17 KB (2,776 words) - 21:07, 7 September 2022
  • have been studied with conventional fluorescence microscopes. However, fluorophores that are bound to the specimen surface and those in the surrounding medium...
    30 KB (3,823 words) - 00:42, 20 March 2024
  • Thumbnail for Shark
    blue light, such as the swell shark and the chain catshark, where the fluorophore derives from a metabolite of kynurenic acid. Sharks have keen olfactory...
    141 KB (14,128 words) - 05:49, 8 May 2024
  • Thumbnail for Förster resonance energy transfer
    distance. Measurements of FRET efficiency can be used to determine if two fluorophores are within a certain distance of each other. Such measurements are used...
    52 KB (6,145 words) - 10:08, 6 January 2024
  • radius (R0): the distance between the fluorophores at which FRET efficiency is 50%. For many FRET fluorophore pairs, R0 lies between 20 and 90 Å, depending...
    10 KB (1,293 words) - 01:37, 25 July 2022
  • Thumbnail for Fluorescence in the life sciences
    acids, lipids or small molecules can be "labelled" with an extrinsic fluorophore, a fluorescent dye which can be a small molecule, protein or quantum...
    27 KB (3,139 words) - 16:37, 3 December 2023
  • fluorescence polarization is the phenomenon where the light emitted by a fluorophore has unequal intensities along different axes of polarization. Early pioneers...
    14 KB (2,043 words) - 10:07, 5 March 2024
  • Thumbnail for Fluorescent tag
    uses a reactive derivative of a fluorescent molecule known as a fluorophore. The fluorophore selectively binds to a specific region or functional group on...
    24 KB (2,775 words) - 15:49, 8 January 2024
  • Thumbnail for Microscopy
    combination of antibodies coupled to a fluorophore as in immunostaining. Examples of commonly used fluorophores are fluorescein or rhodamine. The antibodies...
    69 KB (8,317 words) - 13:29, 18 April 2024