• Thumbnail for Gel electrophoresis of proteins
    specific protein. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques to separate proteins according...
    20 KB (2,351 words) - 06:33, 18 April 2024
  • Thumbnail for Gel electrophoresis
    Gel electrophoresis is a method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and...
    44 KB (5,057 words) - 12:44, 24 April 2024
  • Thumbnail for Agarose gel electrophoresis
    Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a...
    34 KB (4,134 words) - 12:47, 27 September 2023
  • Thumbnail for SDS-PAGE
    gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins...
    39 KB (4,904 words) - 23:35, 23 March 2024
  • Thumbnail for Polyacrylamide gel electrophoresis
    Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology...
    36 KB (4,717 words) - 09:27, 31 January 2024
  • Thumbnail for Two-dimensional gel electrophoresis
    Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins...
    12 KB (1,452 words) - 14:59, 31 December 2023
  • Thumbnail for Electrophoresis
    electrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Gel electrophoresis of proteins History of electrophoresis History...
    16 KB (1,971 words) - 06:18, 29 April 2024
  • Thumbnail for Serum protein electrophoresis
    Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. The most common indications...
    17 KB (2,229 words) - 20:08, 6 February 2024
  • Thumbnail for Temperature gradient gel electrophoresis
    Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature...
    11 KB (1,639 words) - 18:58, 4 February 2024
  • Thumbnail for Electrophoretic mobility shift assay
    (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a...
    9 KB (1,131 words) - 16:07, 17 December 2021
  • Thumbnail for Gel electrophoresis of nucleic acids
    Gel electrophoresis of nucleic acids is an analytical technique to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules are placed...
    24 KB (3,127 words) - 16:34, 26 February 2024
  • Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched...
    3 KB (423 words) - 01:54, 19 July 2023
  • Thumbnail for Affinity electrophoresis
    ligand-binding proteins, one-dimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be...
    19 KB (2,124 words) - 04:22, 8 April 2024
  • Thumbnail for Immunoelectrophoresis
    Immunoelectrophoresis (category Electrophoresis)
    gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel. In contrast to SDS-gel electrophoresis, the electrophoresis in...
    15 KB (1,738 words) - 20:32, 30 October 2023
  • or by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis. Proteins are often purified by using 2D-PAGE and are then analysed...
    30 KB (3,910 words) - 22:02, 25 April 2024
  • Thumbnail for Western blot
    Western blot (category Protein methods)
    preparation, the protein content is ready to be separated by the utilization of gel electrophoresis. The proteins of the sample are separated using gel electrophoresis...
    66 KB (8,047 words) - 01:52, 5 February 2024
  • Thumbnail for Discontinuous electrophoresis
    Discontinuous electrophoresis (colloquially disc electrophoresis) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis...
    4 KB (417 words) - 20:05, 4 February 2024
  • refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric...
    36 KB (4,675 words) - 10:51, 16 January 2024
  • Thumbnail for Agarose
    for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared...
    19 KB (2,406 words) - 16:52, 22 April 2024
  • pulsed-field gel electrophoresis. Electrophoresis Electrophoresis (journal) History of biochemistry History of chromatography History of electrochemistry...
    7 KB (802 words) - 08:24, 22 April 2024
  • QPNC-PAGE (category Electrophoresis)
    Continuous Polyacrylamide Gel Electrophoresis, is a bioanalytical, one-dimensional, high-resolution and high-precision electrophoresis technique applied in...
    33 KB (3,585 words) - 09:03, 24 April 2024
  • Protein methods are the techniques used to study proteins. There are experimental methods for studying proteins (e.g., for detecting proteins, for isolating...
    10 KB (1,069 words) - 18:26, 11 December 2023
  • Thumbnail for Molecular-weight size marker
    on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. Therefore...
    41 KB (4,361 words) - 16:47, 6 April 2024
  • Thumbnail for Electrophoretic color marker
    monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) since DNA, RNA, and most proteins are colourless. The...
    10 KB (1,236 words) - 19:10, 25 March 2024
  • Blood-proteins, also termed plasma proteins, are proteins present in blood plasma. They serve many different functions, including transport of lipids,...
    6 KB (559 words) - 16:06, 22 October 2023
  • Thumbnail for Gel doc
    needed for protein sites with low density. High-abundance proteins are frequently found beside low-abundance proteins on 2DE gels. Because of the fluorescent...
    8 KB (992 words) - 00:11, 9 January 2024
  • Thumbnail for Electroblotting
    Electroblotting (category Electrophoresis)
    to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose, after gel electrophoresis. The protein or nucleic acid can...
    4 KB (395 words) - 02:30, 24 October 2021
  • Thumbnail for Peptide mass fingerprinting
    proteins are reduced and cysteine amino acids are carbamidomethylated chemically or acrylamidated during the gel electrophoresis. Then the proteins are...
    12 KB (1,514 words) - 08:07, 31 March 2024
  • Thumbnail for Comet assay
    DNA migration through the electrophoresis gel, which often resembles a comet. The comet assay (single-cell gel electrophoresis) is a simple method for measuring...
    20 KB (2,499 words) - 09:42, 11 March 2024
  • Thumbnail for Isoelectric focusing
    Isoelectric focusing (category Electrophoresis)
    a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest...
    9 KB (1,172 words) - 09:40, 21 February 2024