A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition...
55 KB (5,853 words) - 06:59, 8 September 2024
restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction...
5 KB (629 words) - 16:22, 23 July 2024
list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of...
80 KB (3,046 words) - 23:44, 27 August 2024
A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria...
8 KB (1,087 words) - 08:23, 12 September 2023
polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences...
9 KB (1,159 words) - 04:36, 23 May 2023
Restriction enzyme mediated integration (abbreviated as REMI) is a technique for integrating DNA (linearised plasmid) into the genome sites that have been...
4 KB (426 words) - 07:50, 22 February 2024
piece using Type IIS restriction enzymes and T4 DNA ligase. This assembly is performed in vitro. Most commonly used Type IIS enzymes include BsaI, BsmBI...
18 KB (2,489 words) - 05:22, 30 April 2024
sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome. Once RAD tags have been isolated...
13 KB (1,424 words) - 22:05, 2 December 2023
declarations Restriction enzyme, a type of enzyme that cleaves genetic material Restriction, a term in medieval supposition theory Restriction (mathematics)...
854 bytes (129 words) - 03:28, 9 September 2024
Palindromic sequence (section Restriction enzyme sites)
palindrome. Many restriction endonucleases (restriction enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes...
7 KB (825 words) - 04:52, 3 June 2024
Thermus aquaticus (section Taq I restriction enzyme)
Thermus aquaticus arose at this time from the convention of giving restriction enzymes short names, such as Sal and Hin, derived from the genus and species...
15 KB (1,792 words) - 04:03, 20 September 2024
Endonuclease (redirect from Endonuclease enzyme)
regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences...
25 KB (2,688 words) - 21:18, 6 April 2024
is designing complementary oligonucleotide sequences that contain restriction enzyme sites along with additional bases on the end that are complementary...
5 KB (683 words) - 08:04, 18 May 2024
such as that borne by bacteriophages. Bacteria have restriction enzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific...
20 KB (2,668 words) - 02:28, 20 June 2024
In a restriction digest, DNA molecules are cleaved at specific restriction sites of 4-12 nucleotides in length by use of restriction enzymes which recognize...
6 KB (784 words) - 02:16, 13 March 2024
EcoRI (category Restriction enzymes)
(pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices...
8 KB (891 words) - 15:42, 23 July 2024
of the two types of enzymes responsible for phage growth restriction in Escherichia coli (E. coli) bacteria. One of these enzymes added a methyl group...
22 KB (2,538 words) - 13:28, 11 September 2024
Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. The quantity...
22 KB (2,935 words) - 02:30, 4 September 2024
In molecular biology, the restriction endonuclease BsobI/AvaI family of enzymes includes the AvaI and BsoBI restriction endonucleases from Anabaena variabilis...
1 KB (102 words) - 20:13, 19 March 2023
from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by using an electric...
17 KB (2,308 words) - 07:37, 31 July 2024
advantages compared to conventional restriction enzyme/ligation cloning of recombinant DNA. For example, No restriction digest of the DNA fragments after...
6 KB (689 words) - 15:42, 24 May 2024
by restriction enzymes that break the molecule's phosphodiester backbone at specific locations, which themselves belong to a larger class of enzymes called...
8 KB (1,091 words) - 08:30, 22 February 2024
Gene mapping (section Restriction mapping)
Restriction mapping is a method in which structural information regarding a segment of DNA is obtained using restriction enzymes. Restriction enzymes...
26 KB (3,363 words) - 22:19, 4 August 2024
purified by size fractionation. Using a second enzyme, DNA ligase, fragments generated by restriction enzymes could be joined in new combinations, termed...
32 KB (4,016 words) - 17:56, 20 August 2024
Enzymes (/ˈɛnzaɪmz/) are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called...
96 KB (9,819 words) - 12:03, 26 July 2024
mixture of PCR amplified variants of a single gene using one or more restriction enzymes and detecting the size of each of the individual resulting terminal...
15 KB (1,966 words) - 06:45, 29 November 2023
to the methylation dependent retention or loss of CpG-containing restriction enzyme sites, such as those for TaqI (TCGA) and BstUI (CGCG), depending on...
12 KB (1,300 words) - 12:11, 5 June 2023
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular...
4 KB (613 words) - 06:50, 30 December 2023
DpnI (category Restriction enzymes)
interactions. DpnII restriction endonuclease family, also isolated from S. pneumonae List of restriction enzyme cutting sites Restriction modification system...
5 KB (514 words) - 13:21, 8 September 2024
most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given: Enzyme: Accepted name...
40 KB (651 words) - 23:27, 19 January 2023