Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions...

Synthetic genetic array Virtual high throughput screening Yeast two-hybrid screening Zhao, Yicheng; Heumueller, Thomas; Zhang, Jiyun; Luo, Junsheng; Kasian...

most conventional and widely used high-throughput methods are yeast two-hybrid screening and affinity purification coupled to mass spectrometry. This system...

the relative levels of the various proteins present in a cell, and two-hybrid screening, which allows the systematic exploration of protein–protein interactions...

yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain is sold commercially by Clontech and is used as a partner...

The GAL4/UAS system is also utilized in Two-Hybrid Screening, a method of identifying interactions between two proteins or a protein with DNA. Gal4 expression...

expression. A more complex use of reporter genes on a large scale is in two-hybrid screening, which aims to identify proteins that natively interact with one...

calcium from the blood and depositing it, in insoluble form, in the bones. The two homeostatic mechanisms working through PTH on the one hand, and calcitonin...

yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech since at least...

transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or...

Dysbindin was discovered by the research group of Derek Blake via yeast two-hybrid screening for binding partners of α-dystrobrevin. In addition, dysbindin is...

blot Southern blot Restriction enzyme Polymerase chain reaction Two-hybrid screening in vivo in vitro in silico Field techniques Belt transect mark and...

gene – locus – trait – allele – polymorphism – homozygote – heterozygote – hybrid – hybridization – dihybrid cross – Punnett square – inbreeding genotype–phenotype...

knockdown of a single gene on the expression of multiple genes. A yeast two-hybrid screening (Y2H) tests a "bait" protein against many potential interacting proteins...

huntingtin interacting protein 1 (HIP1), these were typically found using two-hybrid screening and confirmed using immunoprecipitation. Huntingtin has also been...

under inducing conditions. The broad use of the Gal4 is in yeast two-hybrid screening to screen or to assay protein-protein interactions in eukaryotic...

Jörg; Kern, Rainer; Koegl, Manfred (February 2005). "Automated yeast two-hybrid screening for nuclear receptor-interacting proteins". Molecular & Cellular...

(November 2000). "Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1...

inactivation targeted to the respective tissues. Yeast two-hybrid screening performed by two independent groups identified p0071 (plakophilin-4) as a...

and cause cell-mediated, virus-induced apoptosis. Using a yeast two-hybrid screening system of ORF3 against the porcine cDNA library indicated that the...

human protein encoded by the gene LSMEM1 that were determined via two-hybrid screening (LSMEM2 and MAL) and reconstituted complex (APP) experiments. A reconstituted...

interest to other researchers. Data produced by microarray experiments, two-hybrid screening, and other high-throughput biological experiments is voluminous and...

possible interacting partners. Another early adaptation of yeast two-hybrid screening in protease-substrate discovery is Inactive-catalytic-domain capture...

Fields 1976 – biologist and HHMI investigator known for pioneering two-hybrid screening for discovering protein–protein interactions Edwin James 1816 – botanist...

(November 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs"....

edges. PINs can be discovered with a variety of methods including; Two-hybrid Screening, in vitro: co-immunoprecipitation, blue native gel electrophoresis...

(November 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs"....

thought to interact with the protein encoded by LSMEM1 based on two-hybrid screening. GRCh38: Ensembl release 89: ENSG00000172005 – Ensembl, May 2017...

O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs"....

contains one protein domain of unknown function, DUF1693. Yeast two-hybrid screening has identified three proteins that physically interact with FAM46B...