Microtubule nucleation

In cell biology, microtubule nucleation is the event that initiates de novo formation of microtubules (MTs). These filaments of the cytoskeleton typically form through polymerization of α- and β-tubulin dimers, the basic building blocks of the microtubule, which initially interact to nucleate a seed from which the filament elongates.[1]

Microtubule nucleation occurs spontaneously in vitro, with solutions of purified tubulin giving rise to full-length polymers. The tubulin dimers that make up the polymers have an intrinsic capacity to self-aggregate and assemble into cylindrical tubes, provided there is an adequate supply of GTP. The kinetics barriers of such a process, however, mean that the rate at which microtubules spontaneously nucleate is relatively low.[2]

Role of γ-tubulin and the γ-tubulin ring complex (γ-TuRC)

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In vivo, cells get around this kinetic barrier by using various proteins to aid microtubule nucleation. The primary pathway by which microtubule nucleation is assisted requires the action of a third type of tubulin, γ-tubulin, which is distinct from the α and β subunits that compose the microtubules themselves. The γ-tubulin combines with several other associated proteins to form a conical structure known as the γ-tubulin ring complex (γ-TuRC). This complex, with its 13-fold symmetry, acts as a scaffold or template for α/β tubulin dimers during the nucleation process—speeding up the assembly of the ring of 13 protofilaments that make up the growing microtubule.[3] The γ-TuRC also acts as a cap of the (−) end while the microtubule continues growth from its (+) end. This cap provides both stability and protection to the microtubule (-) end from enzymes that could lead to its depolymerization, while also inhibiting (-) end growth.

MT Nucleation from Microtubule Organizing Centers (MTOCs)

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The γ-TuRC is typically found as the core functional unit in a microtubule organizing center (MTOC), such as the centrosome in some animal cells or the spindle pole bodies in fungi and algae. The γ-TuRCs in the centrosome nucleate an array of microtubules in interphase, which extend their (+)-ends radially outwards into the cytoplasm towards the periphery of the cell. Among its other functions, this radial array is used by microtubule-based motor proteins to transport various cargoes, such as vesicles, to the plasma membrane.

The centrosome is the most common MTOC for multipotent cells in animals, with differentiated tissues utilising a wide variety of non-centrosomal MTOCs.

Non-centrosomal MTOCs

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In animal cells undergoing mitosis, a similar radial array is generated from two MTOCs called the spindle poles, which produce the bipolar mitotic spindle. Some cells however, such as those of higher plants and oocytes, lack distinct MTOCs and microtubules are nucleated via a non-centrosomal pathway. Other cells, such as neurons, skeletal muscle cells, and epithelial cells, which do have MTOCs, possess arrays of microtubules not associated with a centrosome.[4] These non-centrosomal microtubule arrays can take on various geometries—such as those leading to the long, slender shape of myotubes, the fine protrusions of an axon, or the strongly polarized domains of an epithelial cell.

In epithelial cells, CAMSAP3 acts as the non-centrosomal MTOC, and is localised to the apical membrane of the cell.[5] Microtubules grow from this domain in parallel lines, giving the cell its rectangular shape.

The early cells of the pre-implantation mouse embryo utilise a unique non-centrosomal MTOC, in the form of an interphase microtubule bridge joining sister cells. This interphase bridge organises the microtubules of both cells, and uses CAMSAP3 to bind microtubule minus ends.[6]

In the cortical array of plants, as well as in the axons of neurons, it is theorised that microtubules nucleate from existing microtubules via the action of severing enzymes such as katanin.[7] Akin to the action of cofilin in generating actin filament arrays, the severing of microtubules by MAPs creates new plus (+) ends from which microtubules can grow. In this fashion, dynamic arrays of microtubules can be generated without the aid of the γ-TuRC.

Branching MT nucleation

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Studies using Xenopus egg extracts have identified a novel form of microtubule nucleation that generates fan-like branching arrays, in which new microtubules grow at an angle off of older microtubules. These branching microtubules maintain the same polarity as their mother microtubules, and their assembly involves the binding of non-centrosomal γ-TuRCs to the sides of existing microtubules through the augmin complex. This method of microtubule-dependent microtubule nucleation leads to rapid amplification in microtubule density.[8]

Branching MT nucleation has been observed in numerous organisms both in the plant and animal kingdoms. Through use of TIRF microscopy, researchers have visually observed the nucleation of branching microtubules in Drosophila cells during the formation of the mitotic spindle.[9] Five proteins in Drosophila (DGT2 through DGT6) have been identified that are necessary and responsible for facilitating the localization of γ-tubulin to existing MTs and are not associated with its localization at the centrosome.[10]

Role of Microtubule-Associated Proteins (MAPs)

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Though the γ-TuRC is the primary protein used to nucleate microtubules, it is not the only protein that acts as a nucleation factor. Several other MAPs assist the γ-TuRC with the nucleation process, while others nucleate microtubules independently of γ-TuRC. In the branching nucleation described above, the addition of TPX2 to the egg extracts led to a dramatic increase in nucleation events—while in other studies, the protein XMAP215, in vitro, nucleated microtubule asters with its depletion in vivo reducing nucleation potential of centrosomes.[11] The microtubule-binding protein doublecortin, in vitro, nucleates microtubules—acting by binding to the side rather than the end of growing microtubules.[12] Thus, there may be a family of nucleation factor proteins in cells that use a variety of mechanisms to lower the energetic cost of nucleating microtubules. Recent studies have provided evidence towards the concept that the promotion of microtubule nucleation is possible with a combination of α- and β-tubulin dimers and the aforementioned MAP TPX2, even in the absence of γ-TuRC.[13]

Several proteins are involved in formatting the γ-TuRC and temporal and spatial control of microtubule nucleation. These include, for example, coiled-coil proteins with structural functions and regulatory proteins, such as components of the Ran cycle. NEDD1 recruits the γ-TuRC to the centrosome by binding to γ-tubulin.[14][15]

References

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  1. ^ Job D, Valiron O, Oakley B (February 2003). "Microtubule nucleation". Current Opinion in Cell Biology. 15 (1): 111–117. doi:10.1016/S0955-0674(02)00003-0. PMID 12517712.
  2. ^ Desai A, Mitchison TJ (1998). "Microtubule polymerization dynamics". Annual Review of Cell and Developmental Biology. 13: 83–117. doi:10.1146/annurev.cellbio.13.1.83. PMID 9442869.
  3. ^ Kollman JM, Polka JK, Zelter A, Davis TN, Agard DA (August 2010). "Microtubule nucleating gamma-TuSC assembles structures with 13-fold microtubule-like symmetry". Nature. 466 (7308): 879–882. Bibcode:2010Natur.466..879K. doi:10.1038/nature09207. PMC 2921000. PMID 20631709.
  4. ^ Bartolini F, Gundersen GG (October 2006). "Generation of noncentrosomal microtubule arrays". Journal of Cell Science. 119 (Pt 20): 4155–4163. doi:10.1242/jcs.03227. PMID 17038542. S2CID 8505948.
  5. ^ Meng, Wenxiang; Mushika, Yoshimi; Ichii, Tetsuo; Takeichi, Masatoshi (November 2008). "Anchorage of Microtubule Minus Ends to Adherens Junctions Regulates Epithelial Cell-Cell Contacts". Cell. 135 (5): 948–959. doi:10.1016/j.cell.2008.09.040. ISSN 0092-8674. PMID 19041755. S2CID 14503414.
  6. ^ Zenker, J.; White, M. D.; Templin, R. M.; Parton, R. G.; Thorn-Seshold, O.; Bissiere, S.; Plachta, N. (September 2017). "A microtubule-organizing center directing intracellular transport in the early mouse embryo". Science. 357 (6354): 925–928. Bibcode:2017Sci...357..925Z. doi:10.1126/science.aam9335. ISSN 0036-8075. PMID 28860385. S2CID 206658036.
  7. ^ Lindeboom JJ, Nakamura M, Hibbel A, Shundyak K, Gutierrez R, Ketelaar T, et al. (December 2013). "A mechanism for reorientation of cortical microtubule arrays driven by microtubule severing". Science. 342 (6163): 1245533. doi:10.1126/science.1245533. PMID 24200811. S2CID 206552196.
  8. ^ Petry S, Groen AC, Ishihara K, Mitchison TJ, Vale RD (February 2013). "Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2". Cell. 152 (4): 768–777. doi:10.1016/j.cell.2012.12.044. PMC 3680348. PMID 23415226.
  9. ^ Verma V, Maresca TJ (September 2019). "Direct observation of branching MT nucleation in living animal cells". The Journal of Cell Biology. 218 (9): 2829–2840. doi:10.1083/jcb.201904114. PMC 6719462. PMID 31340987.
  10. ^ Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, Vale RD, Stuurman N (April 2007). "Genes required for mitotic spindle assembly in Drosophila S2 cells". Science. 316 (5823): 417–421. Bibcode:2007Sci...316..417G. doi:10.1126/science.1141314. PMC 2837481. PMID 17412918.
  11. ^ Popov AV, Severin F, Karsenti E (August 2002). "XMAP215 is required for the microtubule-nucleating activity of centrosomes". Current Biology. 12 (15): 1326–1330. Bibcode:2002CBio...12.1326P. doi:10.1016/s0960-9822(02)01033-3. PMID 12176362.
  12. ^ Bechstedt S, Brouhard GJ (July 2012). "Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends". Developmental Cell. 23 (1): 181–192. doi:10.1016/j.devcel.2012.05.006. PMC 3951992. PMID 22727374.
  13. ^ Sulimenko V, Dráberová E, Dráber P (2022). "γ-Tubulin in microtubule nucleation and beyond". Frontiers in Cell and Developmental Biology. 10: 880761. doi:10.3389/fcell.2022.880761. PMC 9503634. PMID 36158181.
  14. ^ Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A (February 2006). "NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly". The Journal of Cell Biology. 172 (4): 505–515. doi:10.1083/jcb.200510028. PMC 2063671. PMID 16461362.
  15. ^ Manning JA, Shalini S, Risk JM, Day CL, Kumar S (March 2010). "A direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome". PLOS ONE. 5 (3): e9618. Bibcode:2010PLoSO...5.9618M. doi:10.1371/journal.pone.0009618. PMC 2835750. PMID 20224777.
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